Appl. Sci., Volume 10, Issue 8 (April-2 2020) – 351 articles
Cover Story (view full-size image): The stabilization and controlled release of fish bacteria enables the development of oral vaccination. We demonstrated that clay nanoparticles could control the dispersion properties of inactivated bacteria cells as well as the crosslinking degree of the alginate network, expanding the application of conventional Ca2+-alginate beads as fish vaccines with pH-dependent encapsulation–release properties. Two clay nanoparticles, layered double hydroxide (LDH) and kaolinite (KA), with respective positive and negative surface charges, showed a distinct effect in encapsulation and release. The encapsulation efficiency of bacteria was maximized by KA; on the other hand, the release of bacteria in gastric media was most facilitated in the presence of LDH. View this paper.
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