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18 Results Found

  • Article
  • Open Access
3 Citations
3,636 Views
20 Pages

Performance of Recombinant Komagataella phaffii in Plant-Based Meat Flavor Compound-Leghemoglobin (LegH) Production through Fed-Batch Fermentations

  • Arturs Suleiko,
  • Konstantins Dubencovs,
  • Andris Kazaks,
  • Anastasija Suleiko,
  • Janis Edmunds Daugavietis,
  • Elina Didrihsone,
  • Janis Liepins,
  • Emils Bolmanis,
  • Oskars Grigs and
  • Juris Vanags

Soy leghemoglobin (LegH) has been gaining interest over the last years as an efficient flavor and aroma compound in plant-based meat substitutes. Hence, in the following article, we demonstrate the methods for LegH production using a recombinant Koma...

  • Article
  • Open Access
3 Citations
2,373 Views
12 Pages

20 April 2025

Leghemoglobin (LegHb) is a plant-derived heme-containing globin found in the root nodules of legumes like soybean that can be used as a food additive for red color and meaty flavor as a plant-based meat alternative. However, conventional extraction m...

  • Article
  • Open Access
12 Citations
4,670 Views
19 Pages

Production and Analytical Aspects of Natural Pigments to Enhance Alternative Meat Product Color

  • Allah Bakhsh,
  • Changjun Cho,
  • Kei Anne Baritugo,
  • Bosung Kim,
  • Qamar Ullah,
  • Attaur Rahman and
  • Sungkwon Park

17 March 2023

Color is a major feature that strongly influences the consumer’s perception, selection, and acceptance of various foods. An improved understanding regarding bio-safety protocols, health welfare, and the nutritional importance of food colorants...

  • Article
  • Open Access
18 Citations
5,311 Views
20 Pages

Expressed Soybean Leghemoglobin: Effect on Escherichia coli at Oxidative and Nitrosative Stress

  • Olga V. Kosmachevskaya,
  • Elvira I. Nasybullina,
  • Konstantin B. Shumaev and
  • Alexey F. Topunov

28 November 2021

Leghemoglobin (Lb) is an oxygen-binding plant hemoglobin of legume nodules, which participates in the symbiotic nitrogen fixation process. Another way to obtain Lb is its expression in bacteria, yeasts, or other organisms. This is promising for both...

  • Article
  • Open Access
841 Views
13 Pages

2 October 2025

Leghemoglobin (LegH), a plant-derived hemoprotein, is engineered for the first time as a standalone biocatalyst for carbene N–H insertion. Through semi-rational design, the K65P mutation in the heme pocket significantly enhances catalytic effic...

  • Article
  • Open Access
6 Citations
3,852 Views
14 Pages

Efficient Secretory Expression of Leghemoglobin in Saccharomyces cerevisiae

  • Yiyun Huang,
  • Jingwen Zhou,
  • Jianghua Li,
  • Guocheng Du,
  • Jian Chen and
  • Xinrui Zhao

Leghemoglobin (LegH) is a plant-derived hemoglobin that can be used as a food additive to confer red color and meat flavor to plant-based meat products. Although LegH has been expressed in Saccharomyces cerevisiae, the productivity is low at the shak...

  • Article
  • Open Access
42 Citations
5,734 Views
15 Pages

10 October 2019

Nitrogen (N) in soybean (Glycine max L.) plants derived from biological nitrogen fixation was shown to be a sustainable N resource to substitute for N fertilizer. However, the limited water supply in sandy soil is a critical factor for soybean nodula...

  • Article
  • Open Access
16 Citations
7,500 Views
23 Pages

Enhanced Synthesis of Antioxidant Enzymes, Defense Proteins and Leghemoglobin in Rhizobium-Free Cowpea Roots after Challenging with Meloydogine incognita

  • Jose T. A. Oliveira,
  • Jose H. Araujo-Filho,
  • Thalles B. Grangeiro,
  • Darcy M. F. Gondim,
  • Jeferson Segalin,
  • Paulo M. Pinto,
  • Celia R. R. S. Carlini,
  • Fredy D. A. Silva,
  • Marina D. P. Lobo and
  • Ilka M. Vasconcelos
  • + 1 author

26 November 2014

The root knot nematodes (RKN), Meloydogine spp., particularly Meloidogyne incognita and Meloidogyne javanica species, parasitize several plant species and are responsible for large annual yield losses all over the world. Only a few available chemical...

  • Article
  • Open Access
12 Citations
4,309 Views
25 Pages

10 February 2023

Soybean (Glycine max) is one of the most important proteins and oilseed crops in the world due to a boom in its demand. In order to meet this demand, various modern agricultural methods are being employed, of which magneto-priming (treatment of seeds...

  • Article
  • Open Access
6 Citations
2,522 Views
20 Pages

25 October 2024

Currently, bacterial inoculant technology focuses on improving long-term storage conditions to ensure adequate rhizobia numbers and their effectiveness as plant growth promoters. This study aimed to investigate whether storage at 4 °C for four ye...

  • Article
  • Open Access
12 Citations
8,680 Views
20 Pages

Production and Purification of Soy Leghemoglobin from Pichia pastoris Cultivated in Different Expression Media

  • Emils Bolmanis,
  • Janis Bogans,
  • Inara Akopjana,
  • Arturs Suleiko,
  • Tatjana Kazaka and
  • Andris Kazaks

12 November 2023

Plant-based meat alternatives, exemplified by Impossible Foods’ Impossible Burger, offer a sustainable, ethical substitute for traditional meat, closely mimicking the taste and appearance of meat by utilizing soy leghemoglobin (LegH), a 16 kDa...

  • Article
  • Open Access
13 Citations
2,417 Views
20 Pages

Effects of Elevated Temperature on Pisum sativum Nodule Development: I—Detailed Characteristic of Unusual Apical Senescence

  • Tatiana A. Serova,
  • Pyotr G. Kusakin,
  • Anna B. Kitaeva,
  • Elena V. Seliverstova,
  • Artemii P. Gorshkov,
  • Daria A. Romanyuk,
  • Vladimir A. Zhukov,
  • Anna V. Tsyganova and
  • Viktor E. Tsyganov

5 December 2023

Despite global warming, the influence of heat on symbiotic nodules is scarcely studied. In this study, the effects of heat stress on the functioning of nodules formed by Rhizobium leguminosarum bv. viciae strain 3841 on pea (Pisum sativum) line SGE w...

  • Article
  • Open Access
16 Citations
3,656 Views
26 Pages

Dynamics of Reactive Carbonyl Species in Pea Root Nodules in Response to Polyethylene Glycol (PEG)-Induced Osmotic Stress

  • Alena Soboleva,
  • Nadezhda Frolova,
  • Kseniia Bureiko,
  • Julia Shumilina,
  • Gerd U. Balcke,
  • Vladimir A. Zhukov,
  • Igor A. Tikhonovich and
  • Andrej Frolov

Drought dramatically affects crop productivity worldwide. For legumes this effect is especially pronounced, as their symbiotic association with rhizobia is highly-sensitive to dehydration. This might be attributed to the oxidative stress, which ultim...

  • Article
  • Open Access
6 Citations
2,980 Views
18 Pages

Role of Nitric Oxide-Derived Metabolites in Reactions of Methylglyoxal with Lysine and Lysine-Rich Protein Leghemoglobin

  • Konstantin B. Shumaev,
  • Olga V. Kosmachevskaya,
  • Elvira I. Nasybullina,
  • Enno K. Ruuge and
  • Alexey F. Topunov

22 December 2022

Carbonyl stress occurs when reactive carbonyl compounds (RCC), such as reducing sugars, dicarbonyls etc., accumulate in the organism. The interaction of RCC carbonyl groups with amino groups of molecules is called the Maillard reaction. One of the mo...

  • Article
  • Open Access
11 Citations
2,784 Views
21 Pages

Harnessing Walnut-Based Zinc Oxide Nanoparticles: A Sustainable Approach to Combat the Disease Complex of Meloidogyne arenaria and Macrophomina phaseolina in Cowpea

  • Mir Akhtar Hussain,
  • Ghazala Parveen,
  • Aashaq Hussain Bhat,
  • Zubair Altaf Reshi,
  • Farid S. Ataya and
  • Zaffar A. Handoo

24 June 2024

Zinc oxide nanoparticles (ZnO NPs) exhibit diverse applications, including antimicrobial, UV-blocking, and catalytic properties, due to their unique structure and properties. This study focused on the characterization of zinc oxide nanoparticles (ZnO...

  • Article
  • Open Access
182 Views
15 Pages

Effects of Calcium Nutrition on Soybean Growth and Symbiotic Nitrogen Fixation

  • Sutong Zhao,
  • Xiaomin Kang,
  • Mingyue Li,
  • Xiaochen Lyu,
  • Chao Yan and
  • Qiulai Song

2 February 2026

Calcium is essential for legume symbiotic nitrogen fixation, acting as both a nutrient and a signal. Yet, how varying calcium levels—from deficiency to toxicity—affect the soybean ‘root-nodule-stem’ balance has not been fully...

  • Article
  • Open Access
5 Citations
4,750 Views
17 Pages

7 May 2020

In legume crops, formation of developmentally mature nodules is a prerequisite for efficient nitrogen fixation by populations of rhizobial bacteroids established inside nodule cells. Development of root nodules, and concomitant microbial colonization...

  • Article
  • Open Access
4 Citations
3,176 Views
19 Pages

Enhancement of Clover (Trifolium alexandrinum L.) Shade Tolerance and Nitrogen Fixation under Dense Stands-Based Cropping Systems

  • Wael Toukabri,
  • Nouha Ferchichi,
  • Meriem Barbouchi,
  • Dorsaf Hlel,
  • Mohamed Jadlaoui,
  • Haithem Bahri,
  • Ridha Mhamdi,
  • Hatem Cheikh M’hamed,
  • Mohamed Annabi and
  • Darine Trabelsi

28 September 2022

Improving legumes crops’ performance under dense stands shade environment (e.g., intercropped oats–clover) is needed to promote agroecological practices. Previous studies have revealed that ethylene produced by plants under dense standing...