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Article

Expressed Soybean Leghemoglobin: Effect on Escherichia coli at Oxidative and Nitrosative Stress

Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, Russia
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Academic Editor: Maurizio Battino
Molecules 2021, 26(23), 7207; https://doi.org/10.3390/molecules26237207
Received: 25 October 2021 / Revised: 22 November 2021 / Accepted: 24 November 2021 / Published: 28 November 2021
Leghemoglobin (Lb) is an oxygen-binding plant hemoglobin of legume nodules, which participates in the symbiotic nitrogen fixation process. Another way to obtain Lb is its expression in bacteria, yeasts, or other organisms. This is promising for both obtaining Lb in the necessary quantity and scrutinizing it in model systems, e.g., its interaction with reactive oxygen (ROS) and nitrogen (RNS) species. The main goal of the work was to study how Lb expression affected the ability of Escherichia coli cells to tolerate oxidative and nitrosative stress. The bacterium E. coli with the embedded gene of soybean leghemoglobin a contains this protein in an active oxygenated state. The interaction of the expressed Lb with oxidative and nitrosative stress inducers (nitrosoglutathione, tert-butyl hydroperoxide, and benzylviologen) was studied by enzymatic methods and spectrophotometry. Lb formed NO complexes with heme-nitrosylLb or nonheme iron-dinitrosyl iron complexes (DNICs). The formation of Lb-bound DNICs was also detected by low-temperature electron paramagnetic resonance spectroscopy. Lb displayed peroxidase activity and catalyzed the reduction of organic peroxides. Despite this, E. coli-synthesized Lb were more sensitive to stress inducers. This might be due to the energy demand required by the Lb synthesis, as an alien protein consumes bacterial resources and thereby decreases adaptive potential of E. coli. View Full-Text
Keywords: leghemoglobin; Escherichia coli; protein expression; dinitrosyl iron complexes; spectrophotometry; electron paramagnetic resonance; oxidative stress; nitrosative stress leghemoglobin; Escherichia coli; protein expression; dinitrosyl iron complexes; spectrophotometry; electron paramagnetic resonance; oxidative stress; nitrosative stress
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MDPI and ACS Style

Kosmachevskaya, O.V.; Nasybullina, E.I.; Shumaev, K.B.; Topunov, A.F. Expressed Soybean Leghemoglobin: Effect on Escherichia coli at Oxidative and Nitrosative Stress. Molecules 2021, 26, 7207. https://doi.org/10.3390/molecules26237207

AMA Style

Kosmachevskaya OV, Nasybullina EI, Shumaev KB, Topunov AF. Expressed Soybean Leghemoglobin: Effect on Escherichia coli at Oxidative and Nitrosative Stress. Molecules. 2021; 26(23):7207. https://doi.org/10.3390/molecules26237207

Chicago/Turabian Style

Kosmachevskaya, Olga V., Elvira I. Nasybullina, Konstantin B. Shumaev, and Alexey F. Topunov. 2021. "Expressed Soybean Leghemoglobin: Effect on Escherichia coli at Oxidative and Nitrosative Stress" Molecules 26, no. 23: 7207. https://doi.org/10.3390/molecules26237207

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