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30 Results Found

  • Article
  • Open Access
16 Citations
9,720 Views
19 Pages

Electrochemical Genotoxicity Assay Based on a SOS/umu Test Using Hydrodynamic Voltammetry in a Droplet

  • Hideki Kuramitz,
  • Kazuto Sazawa,
  • Yasuaki Nanayama,
  • Noriko Hata,
  • Shigeru Taguchi,
  • Kazuharu Sugawara and
  • Masami Fukushima

14 December 2012

The SOS/umu genotoxicity assay evaluates the primary DNA damage caused by chemicals from the β-galactosidase activity of S. typhimurium. One of the weaknesses of the common umu test system based on spectrophotometric detection is that it is unable to...

  • Article
  • Open Access
1,200 Views
11 Pages

A Pipette for High-Resolution Sampling and Delivery of pL Bio-Samples

  • Ziyang Han,
  • Pengfei Gong,
  • Hengxiang Su,
  • Zehang Gao,
  • Shilun Feng and
  • Jianlong Zhao

Conventional laboratory methods for handling valuable biological samples typically use pipettes or needles, which are prone to issues such as dead volume and sample waste. Furthermore, the sampling and processing of pathogenic bacteria, such as Esche...

  • Article
  • Open Access
12 Citations
9,556 Views
13 Pages

4 March 2015

Soil enzymes play essential roles in catalyzing reactions necessary for nutrient cycling in the biosphere. They are also sensitive indicators of ecosystem stress, therefore their evaluation is very important in assessing soil health and quality. The...

  • Article
  • Open Access
571 Views
18 Pages

23 October 2025

Hundred-micron-sized microdroplets are widely used in microbial culture, chemical investigations and industrial processes. The size, velocity and frequency of microdroplets significantly affect the cultivation and processing effects. The detections o...

  • Article
  • Open Access
6 Citations
4,255 Views
13 Pages

15 June 2023

A device and method for the constant pressure regulation of microdroplet PCR in microfluidic chips are developed to optimize for the microdroplet movement, fragmentation, and bubble generation in microfluidic chips. In the developed device, an air so...

  • Article
  • Open Access
5 Citations
5,611 Views
8 Pages

High-Pressure Acceleration of Nanoliter Droplets in the Gas Phase in a Microchannel

  • Yutaka Kazoe,
  • Ippei Yamashiro,
  • Kazuma Mawatari and
  • Takehiko Kitamori

15 August 2016

Microfluidics has been used to perform various chemical operations for pL–nL volumes of samples, such as mixing, reaction and separation, by exploiting diffusion, viscous forces, and surface tension, which are dominant in spaces with dimensions on th...

  • Article
  • Open Access
9 Citations
5,294 Views
12 Pages

28 April 2020

In recent years, the development of polymerase chain reaction (PCR) technology has focused on digital PCR, which depends on the microfluidics. Based on continuous-flow microfluidic technology, this paper designed a miniaturized digital PCR amplificat...

  • Article
  • Open Access
5 Citations
4,369 Views
12 Pages

Irregular Antibody Screening Using a Microdroplet Platform

  • Ding-Ping Chen,
  • Pei-Yu Wu and
  • Yen-Heng Lin

4 September 2023

The screening procedure for antibodies is considered the most tedious among the three pretransfusion operations, i.e., ABO and Rhesus (Rh) typing, irregular antibody screening/identification, and crossmatching tests. The commonly used screening metho...

  • Article
  • Open Access
4 Citations
3,097 Views
13 Pages

On-Chip Nucleic Acid Purification Followed by ddPCR for SARS-CoV-2 Detection

  • Cong Ma,
  • Yimeng Sun,
  • Yuhang Huang,
  • Zehang Gao,
  • Yaru Huang,
  • Ikshu Pandey,
  • Chunping Jia,
  • Shilun Feng and
  • Jianlong Zhao

We developed a microfluidic chip integrated with nucleic acid purification and droplet-based digital polymerase chain reaction (ddPCR) modules to realize a ‘sample-in, result-out’ infectious virus diagnosis. The whole process involved pul...

  • Article
  • Open Access
6 Citations
2,951 Views
15 Pages

Development of an Inkjet Setup for Printing and Monitoring Microdroplets

  • Beatriz Cavaleiro de Ferreira,
  • Tiago Coutinho,
  • Miguel Ayala Botto and
  • Susana Cardoso

31 October 2022

Inkjet printing is a digitally controlled additive technology that allows the precise deposition of droplets. Because it is additive, it enables geometries usually unattainable by other technologies. Because it is digitally controlled, its output is...

  • Communication
  • Open Access
16 Citations
6,680 Views
15 Pages

Lignin as a Functional Green Coating on Carbon Fiber Surface to Improve Interfacial Adhesion in Carbon Fiber Reinforced Polymers

  • László Szabó,
  • Sari Imanishi,
  • Fujie Tetsuo,
  • Daisuke Hirose,
  • Hisai Ueda,
  • Takayuki Tsukegi,
  • Kazuaki Ninomiya and
  • Kenji Takahashi

6 January 2019

While intensive efforts are made to prepare carbon fiber reinforced plastics from renewable sources, less emphasis is directed towards elaborating green approaches for carbon fiber surface modification to improve the interfacial adhesion in these com...

  • Article
  • Open Access
1 Citations
2,330 Views
11 Pages

Single Microdroplet Breakup-Assisted Viscosity Measurement

  • Yeongseok Jang,
  • Hwabok Wee,
  • Jonghyun Oh and
  • Jinmu Jung

31 March 2022

Recently, with the development of biomedical fields, the viscosity of prepolymer fluids, such as hydrogels, has played an important role in determining the mechanical properties of the extracellular matrix (ECM) or being closely related to cell viabi...

  • Communication
  • Open Access
1 Citations
4,464 Views
9 Pages

External-Voltage-Free Dielectrophoresis of Liquid Crystal Droplets

  • Sheng-Kuang Wu,
  • Ting-Shan Mo,
  • Jia-De Lin,
  • Shuan-Yu Huang,
  • Hui-Chen Yeh,
  • Lin-Jer Chen and
  • Chia-Rong Lee

3 July 2017

This work reports, for the first time, a dielectrophoresis (DEP) effect-induced motion of liquid crystal (LC) droplets in an LC/monomer mixture sample with a poly-(N-vinyl carbazole) PVK-coated substrate without an external voltage. With the UV pre-i...

  • Article
  • Open Access
30 Citations
6,676 Views
12 Pages

Standardized Sample Preparation Using a Drop-on-Demand Printing Platform

  • Ellen L. Holthoff,
  • Mikella E. Farrell and
  • Paul M. Pellegrino

7 May 2013

Hazard detection systems must be evaluated with appropriate test material concentrations under controlled conditions in order to accurately identify and quantify unknown residues commonly utilized in theater. The existing assortment of hazard referen...

  • Review
  • Open Access
166 Citations
18,924 Views
20 Pages

Recent Advances and Future Perspectives on Microfluidic Liquid Handling

  • Nam-Trung Nguyen,
  • Majid Hejazian,
  • Chin Hong Ooi and
  • Navid Kashaninejad

12 June 2017

The interdisciplinary research field of microfluidics has the potential to revolutionize current technologies that require the handling of a small amount of fluid, a fast response, low costs and automation. Microfluidic platforms that handle small am...

  • Article
  • Open Access
4 Citations
6,019 Views
10 Pages

Micro-Droplet Platform for Exploring the Mechanism of Mixed Field Agglutination in B3 Subtype

  • Ding-Ping Chen,
  • Chen Chen,
  • Pei-Yu Wu,
  • Yen-Heng Lin,
  • Wei-Tzu Lin and
  • Yi-Liang Yan

16 August 2021

B3 is the most common subtype of blood group B in the Taiwanese population, and most of the B3 individuals in the Taiwanese population have the IVS3 + 5 G > A (rs55852701) gene variation. Additionally, a typical mixed field agglutination is observ...

  • Article
  • Open Access
4 Citations
2,942 Views
15 Pages

Improvement of the Surface Properties of Polyether Ether Ketone via Arc Evaporation for Biomedical Applications

  • Alexander Y. Fedotkin,
  • Igor O. Akimchenko,
  • Tuan-Hoang Tran,
  • Artur R. Shugurov,
  • Evgeniy V. Shesterikov,
  • Anna I. Kozelskaya,
  • Sven Rutkowski and
  • Sergei I. Tverdokhlebov

9 April 2023

Polyether ether ketone is a bioinert polymer, that is of high interest in research and medicine as an alternative material for the replacement of bone implants made of metal. The biggest deficit of this polymer is its hydrophobic surface, which is ra...

  • Article
  • Open Access
2,109 Views
10 Pages

19 September 2024

An electrochemical bioassay based on rotating droplet electrochemistry by using an electron-transfer mediator was developed for the evaluation of a wide variety of pollutants such as antibiotics, heavy metals, and pesticides in the water environment....

  • Article
  • Open Access
456 Views
17 Pages

Analysis of Niobium and Stainless Steel Electropolishing Solutions by Laser-Induced Breakdown Spectroscopy Using a Porous Silicon Substrate and a Non-Contact Pretreatment

  • Ayumu Matsumoto,
  • Yuki Takeda,
  • Kiichi Kuroda,
  • Hiroto Torigoe,
  • Yui Sugita,
  • Yusuke Shimazu,
  • Keisuke Nii,
  • Yoshiaki Ida and
  • Shinji Yae

6 February 2026

Electropolishing is an essential process for the surface treatment of metallic materials. To determine the appropriate replacement timing of electropolishing solutions for their efficient use and improved productivity, it is important to periodically...

  • Article
  • Open Access
8 Citations
2,302 Views
15 Pages

An In Situ Formation of Ionic Liquid for Enrichment of Triazole Fungicides in Food Applications Followed by HPLC Determination

  • Rawikan Kachangoon,
  • Jitlada Vichapong,
  • Yanawath Santaladchaiyakit and
  • Supalax Srijaranai

An in situ formation of ionic liquid was used for preconcentration of four triazole fungicides in food samples. The microextraction method was used for the first time in the literature for preconcentration of triazole fungicides. In the developed met...

  • Article
  • Open Access
8 Citations
3,001 Views
20 Pages

15 December 2021

To function as source control, a fabric mask must be able to filter micro-droplets (≥5 µm) in expiratory secretions and still allow the wearer to breathe normally. This study investigated the effects of fabric structural properties on the fi...

  • Article
  • Open Access
2 Citations
2,726 Views
12 Pages

18 September 2023

Uniform and stable droplet generation is critical for accurate and efficient digital nucleic acid analysis (dNAA). In this study, an integrated microfluidic step emulsification device with wide-range droplet generation capability, small device dimens...

  • Feature Paper
  • Article
  • Open Access
12 Citations
3,591 Views
19 Pages

Corrosion Improvement of 304L Stainless Steel by ZrSiN and ZrSi(N,O) Mono- and Double-Layers Prepared by Reactive Cathodic Arc Evaporation

  • Mihaela Dinu,
  • Anca Constantina Parau,
  • Alina Vladescu,
  • Adrian Emil Kiss,
  • Iulian Pana,
  • Emile S. Massima Mouele,
  • Leslie Felicia Petrik and
  • Viorel Braic

15 October 2021

Zr-based nitrides and oxynitrides were deposited by reactive cathodic arc evaporation in monolayer and double-layer structures with the aim of increasing the corrosion protection of 304L stainless steel (SS) in a biomedical aggressive environment. Al...

  • Article
  • Open Access
2 Citations
1,998 Views
12 Pages

15 November 2024

The weakness of the fiber–matrix interface restricts the practical application of basalt fiber (BF) as a reinforcing material. In order to improve the interfacial adhesion between the BF and epoxy matrix, surface activation of the BF was carrie...

  • Article
  • Open Access
1 Citations
4,365 Views
17 Pages

Moderate Signal Enhancement in Electrospray Ionization Mass Spectrometry by Focusing Electrospray Plume with a Dielectric Layer around the Mass Spectrometer’s Orifice

  • Zi Qing Chua,
  • Gurpur Rakesh D. Prabhu,
  • Yi-Wun Wang,
  • Chamarthi Maheswar Raju,
  • Krzysztof Buchowiecki,
  • Ochir Ochirov,
  • Decibel P. Elpa and
  • Pawel L. Urban

8 January 2024

Electrospray ionization (ESI) is among the commonly used atmospheric pressure ionization techniques in mass spectrometry (MS). One of the drawbacks of ESI is the formation of divergent plumes composed of polydisperse microdroplets, which lead to low...

  • Article
  • Open Access
7 Citations
2,920 Views
13 Pages

Pulsed Laser Deposition of SWCNTs on Carbon Fibres: Effect of Deposition Temperature

  • Călin Moise,
  • Lidar Rachmani,
  • Geanina Mihai,
  • Oana Lazar,
  • Marius Enăchescu and
  • Naum Naveh

2 April 2021

Single wall carbon nanotubes (SWCNTs) were grown on either sized or desized carbon fabric in a self-designed reactor by Pulsed Laser Deposition (PLD). The uniqueness of the PLD system lies, among other things, in the ability to keep the substrate at...

  • Article
  • Open Access
15 Citations
2,837 Views
29 Pages

10 January 2023

In life-testing investigations, accelerated life testing is crucial since it reduces both time and costs. In this study, constant-stress partially accelerated life tests using adaptive progressively Type I censored samples are taken into account. Thi...

  • Article
  • Open Access
15 Citations
5,798 Views
13 Pages

The Formation of Composite Ti-Al-N Coatings Using Filtered Vacuum Arc Deposition with Separate Cathodes

  • Ivan A. Shulepov,
  • Egor B. Kashkarov,
  • Igor B. Stepanov,
  • Maxim S. Syrtanov,
  • Alina N. Sutygina,
  • Ivan Shanenkov,
  • Aleksei Obrosov and
  • Sabine Weiß

12 November 2017

Ti-Al-N coatings were deposited on high-speed steel substrates by filtered vacuum arc deposition (FVAD) during evaporation of aluminum and titanium cathodes. Distribution of elements, phase composition, and mechanical properties of Ti-Al-N coatings w...

  • Article
  • Open Access
261 Views
12 Pages

4 February 2026

This paper proposes and experimentally validates a label-free microdroplet concentration detector based on a quad-resonator metamaterial. The device exploits the linear relationship between the dielectric constant of a binary mixed solution and its c...

  • Article
  • Open Access
768 Views
15 Pages

The Effect of Cr Cathode Arc Current on the Wear Resistance of Cr/(Zr,Cr)N/(Zr,Cr,Al)N Coatings on 7050 Aluminum Alloy

  • Peiyu He,
  • Tao He,
  • Xiangyang Du,
  • Alexey Vereschaka,
  • Catherine Sotova,
  • Jian Li,
  • Yang Ding,
  • Kang Chen and
  • Yuqi Wang

15 September 2025

The application of 7050 aluminum alloy in high-friction environments is limited due to its insufficient surface wear resistance. This study aims to enhance its wear resistance by depositing Cr/(Zr,Cr)N/(Zr,Cr,Al)N multilayer composite coatings using...