Search Results (12)

Staphylococcus aureus is a vital pathogen causing clinical infections. Capsules are important virulence factors for S. aureus. This study investigates the regulatory mechanisms underlying capsule production in S. aureus. Bacterial strains XN108 and Newman were used, and combined approaches like RNA sequencing (RNA-seq), RT-qPCR, transmission electron microscopy (TEM), gene reporter, and electrophoretic mobility shift assay (EMSA) were performed to test the role and mechanism of WalK(S221P) mutation in S. aureus capsule production. RNA-seq showed an increased expression of cap genes in the WalK(S221P)-carried S. aureus XN108 relative to the mutation-cured XN108-R. TEM and capsular polysaccharide determination demonstrated that XN108 produced more capsules than XN108-R did. Similar results were presented in the WalK(S221P)-contained K-Newman versus the wild-type Newman. RT-qPCR screening showed an increasing expression of the mgrA gene in XN108 versus XN108-R. Gene reporter and EMSA analysis revealed that WalK(S221P) mutation promoted S. aureus capsule production through MgrA. Deletion of mgrA decreased the WalK(S221P)-mediated capsule yield. Moreover, WalK(S221P) mutation remarkably increased the tolerance of S. aureus to whole blood killing and microphage phagocytosis. Overall, these data provide mechanistic insights into the effect of WalK(S221P) on the capsule production of S. aureus, which may set down foundations for future S. aureus virulence investigations. Full article

Recent evidence indicates that the gut microbiota (GM) has a significant impact on the inflammatory bowel disease (IBD) progression. Our aim was to investigate the GM profiles, the Microbial Dysbiosis Index (MDI) and the intestinal microbiota-associated markers in relation to IBD clinical characteristics and disease state. We performed 16S rRNA metataxonomy on both stools and ileal biopsies, metabolic dysbiosis tests on urine and intestinal permeability and mucosal immunity activation tests on the stools of 35 IBD paediatric patients. On the GM profile, we assigned the MDI to each patient. In the statistical analyses, the MDI was correlated with clinical parameters and intestinal microbial-associated markers. In IBD patients with high MDI, Gemellaceae and Enterobacteriaceae were increased in stools, and Fusobacterium, Haemophilus and Veillonella were increased in ileal biopsies. Ruminococcaceae and WAL_1855D were enriched in active disease condition; the last one was also positively correlated to MDI. Furthermore, the MDI results correlated with PUCAI and Matts scores in ulcerative colitis patients (UC). Finally, in our patients, we detected metabolic dysbiosis, intestinal permeability and mucosal immunity activation. In conclusion, the MDI showed a strong association with both severity and activity of IBD and a positive correlation with clinical scores, especially in UC. Thus, this evidence could be a useful tool for the diagnosis and prognosis of IBD. Full article

Raw material with a high Al₂O₃ content has led to an increase in the Al₂O₃ content in blast furnace slag, which has affected the normal operation of a blast furnace. The exergy analysis method is an important method for studying the energy utilization of high-alumina blast furnace smelting. In this paper, to investigate the impact of slag composition on exergy efficiency and optimize exergy efficiency during the smelting process of high Al₂O₃ iron ore, a gray box exergy analysis model of blast furnace smelting and an objective function for minimizing the total exergy loss were developed. The results indicated that the blast furnace smelting process had an exergy efficiency (η) of 28.29% for hot metal and slag; the exergy efficiency of the blast furnace did not significantly increase with the increasing w(MgO)/w(Al₂O₃) and R (w(CaO)/w(SiO₂)), but the exergy efficiency of the blast furnace declined with increasing w(Al₂O₃). The regional optimal solution for the objective function method was 7129.42 MJ with slag compositions of R = 1.295, w(MgO)/w(Al₂O₃) = 0.545, and w(Al₂O₃) = 15%. Full article

Bacterial blight, caused by Xanthomonas arboricola pv. juglandis, is a significant disease affecting walnut production worldwide. Outbreaks are most severe in spring, and closely tied to host phenology and weather conditions. Pathogen infections are mainly observed in catkins, female flowers, leaves, and fruits. In this study, the effect of wetness duration and temperature on walnut infections by X. arboricola pv. juglandis was determined through two independent experiments conducted under controlled environmental conditions. The combined effect of both climatic parameters on disease severity was quantified using a third-order polynomial equation. The model obtained by linear regression and backward elimination technique fitted well to the data (R² = 0.94 and R²_adj = 0.93). The predictive capacity of the forecasting model was evaluated on pathogen-inoculated walnut plants exposed to different wetness duration–temperature combinations under Mediterranean field conditions. Observed disease severity in all events aligned with predicted infection risk. Additionally, the relationship between leaf and fruit age and the disease severity was quantified and modelled. A prediction model, which has been referred to as the WalBlight-risk model, is proposed for evaluation as an advisory system for timing bactericide sprays to manage bacterial blight in Mediterranean walnut orchards. Full article

The emergence of antibiotic-resistant S. aureus has become a major public health concern, necessitating the discovery of new antimicrobial compounds. Given that the skin microbiome plays a critical role in the host defence against pathogens, the development of therapies that target the interactions between commensal bacteria and pathogens in the skin microbiome offers a promising approach. Here, we report the discovery of two bacteriocins, cerein 7B and cerein B4080, that selectively inhibit S. aureus without affecting S. epidermidis, a commensal bacterium on the skin. Our study revealed that exposure of S. aureus to these bacteriocins resulted in mutations in the walK/R two-component system, leading to a thickening of the cell wall visible by transmission electron microscopy and subsequent decreased sensitivity to vancomycin. Our findings prompt a nuanced discussion of the potential of those bacteriocins for selective targeting of S. aureus on the skin, given the emergence of resistance and co-resistance with vancomycin. The idea put forward implies that by preserving commensal bacteria, selective compounds could limit the emergence of resistance in pathogenic cells by promoting competition with remaining commensal bacteria, ultimately reducing chronical infections and limiting the spread of antibiotic resistance. Full article

The interaction between water and land resources (WALRs) has been further enhanced with the development of human production activities. Evaluating the synergetic development (SD) level of WALRs is conducive to discovering the weakness of comprehensive utilization of resources and promoting sustainable development. However, previous studies did not clearly elucidate the effects of the synergetic development between WALRs (SD-WALRs). For evaluation methods, the impact of various subsystem development levels on the whole system is often ignored due to its unclear definition. Therefore, in this research, the concept of the SD-WALRs was defined based on synergetic theory. By using the “Driving Force–Pressure–State–Impact–Response” (DPSIR) model, comprehensive evaluation index systems of WALR development were established. The index systems were regarded as efficacy functions of the coupling coordination degree (CCD) model, and the evaluation model of the SD-WALR level was constructed based on it. Taking Luoyang City as an example, using the latest 10 years (2010–2019) with available data as the study period, the results showed that the value of the SD degree increased from 0.609 to 0.789 during the study period, which reached the level of intermediate synergetic development. In general, the development of WALRs showed a positive trend from “high coupling but low synergetic development” to “high synergetic development”. Full article

Fecal microbiome culturomics of a cancer patient treated with immune checkpoint inhibitors led to the identification of a Gram-negative, rod-shaped, obligate anaerobic, non-motile, non-spore-forming bacterium, designated strain kh20^T, which was phylogenetically assigned to the genus Alistipes. Strain kh20^T demonstrated a 98.61% 16S rRNA sequence similarity with A.shahii WAL 8301^T. The bacteria cells generated catalase but no oxidase. Iso-C_15:0 (26.6%), anteiso-C_15:0 (19.9%), and iso-C_17:0 (17.2%) were the major cellular fatty acids identified in its composition. The G+C content of its genome was 57.2%. Strain kh20^T showed significantly low values for DNA-DNA Hybridation (DDH ≤ 33.70%) and Average Nucleotide Identity (ANI ≤ 86.35%) compared with other Alistipes species. Based on these findings, we concluded that strain kh20^T represented a novel bacterium, and we proposed the name Alistipes montrealensis sp. nov. (CECT 30384 and CSUR Q6005). Full article

Composition of phytopathogens—causal agents of “black point” was studied in 2018–2019. Investigations were carried out at the Institute of Plant Protection of National Academy of Agrarian Sciences of Ukraine. Seed samples were taken from different locations in the Forest-Steppe and Polissya zones of Ukraine. The results obtained showed that fungi from the genus Alternaria Nees dominated in the pathogen complex. They were isolated from 76.5–83.1% of seeds from the Forest-Steppe of Ukraine. Less often they were found in samples from the Polissya zone (59.0–69.4%). Alternaria tenuissima тa Alternaria infectoria were isolated in an almost equal proportion. In addition, seeds with “black point” were colonized by fungi from genera Fusarium Link, Curvularia Boed, Bipolaris Shoem., Aspergillus P.Micheli, Nigrospora Zimm., Cladosporium Link, Acremoniella Sacc., Stemphillium Walr., Sordaria Ces. & De Not, Epicoccum Link, Aureobasidium Viala at Boy. It should be noted that the causal agent of nigrospora dry rot of maize Nigrospora oryzae (Berk & Broom) Petch was isolated from nearly 6% of seeds from the Polissya zone. In addition, saprophitic fungi Stemphillium spp. and Sordaria spp. were found. The majority of isolated fungi produce mycotoxins which are harmful for people and animals. Thus, the monitoring of species composition of pathogens on wheat seeds, in particular causal agents of “black point”, is essential for obtaining a high-quality wheat grain. Full article

Objective: To provide the quantitative volumetric data of the total lung and lobes in inspiration and expiration from healthy adults, and to explore the value of paired inspiratory–expiratory chest CT scan in pulmonary ventilatory function and further explore the influence of each lobe on ventilation. Methods: A total of 65 adults (29 males and 36 females) with normal clinical pulmonary function test (PFT) and paired inspiratory–expiratory chest CT scan were retrospectively enrolled. The inspiratory and expiratory volumetric indexes of the total lung (TL) and 5 lobes (left upper lobe [LUL], left lower lobe [LLL], right upper lobe [RUL], right middle lobe [RML], and right lower lobe [RLL]) were obtained by Philips IntelliSpace Portal image postprocessing workstation, including inspiratory lung volume (LV_in), expiratory lung volume (LV_ex), volume change (∆LV), and well-aerated lung volume (WAL, lung tissue with CT threshold between −950 and −750 HU in inspiratory scan). Spearman correlation analysis was used to explore the correlation between CT quantitative indexes of the total lung and ventilatory function indexes (including total lung capacity [TLC], residual volume [RV], and force vital capacity [FVC]). Multiple stepwise regression analysis was used to explore the influence of each lobe on ventilation. Results: At end-inspiratory phase, the LV_in-TL was 4664.6 (4282.7, 5916.2) mL, the WAL_TL was 4173 (3639.6, 5250.9) mL; both showed excellent correlation with TLC (LV_in-TL: r = 0.890, p < 0.001; WAL_TL: r = 0.879, p < 0.001). From multiple linear regression analysis with lobar CT indexes as variables, the LV_in and WAL of these two lobes, LLL and RUL, showed a significant relationship with TLC. At end-expiratory phase, the LV_ex-TL was 2325.2 (1969.7, 2722.5) mL with good correlation with RV (r = 0.811, p < 0.001), of which the LV_ex of RUL and RML had a significant relationship with RV. For the volumetric change within breathing, the ∆LV_TL was 2485.6 (2169.8, 3078.1) mL with good correlation with FVC (r = 0.719, p < 0.001), moreover, WAL_TL showed a better correlation with FVC (r = 0.817, p < 0.001) than that of ∆LV_TL. Likewise, there was also a strong association between ∆LV, WAL of these two lobes (LLL and RUL), and FVC. Conclusions: The quantitative indexes derived from paired inspiratory–expiratory chest CT could reflect the clinical pulmonary ventilatory function, LLL, and RUL give greater impact on ventilation. Thus, the pulmonary functional evaluation needs to be more precise and not limited to the total lung level. Full article

Substantial uncertainties are associated with satellite precipitation datasets (SPDs), which are further amplified over complex terrain and diverse climate regions. The current study develops a regional blended precipitation dataset (RBPD) over Pakistan from selected SPDs in different regions using a dynamic weighted average least squares (WALS) algorithm from 2007 to 2018 with 0.25° spatial resolution and one-day temporal resolution. Several SPDs, including Global Precipitation Measurement (GPM)-based Integrated Multi-Satellite Retrievals for GPM (IMERG), Tropical Rainfall Measurement Mission (TRMM) Multi-Satellite Precipitation Analysis (TMPA) 3B42-v7, Precipitation Estimates from Remotely Sensed Information Using Artificial Neural Networks-Climate Data Record (PERSIANN-CDR), ERA-Interim (reanalysis dataset), SM2RAIN-CCI, and SM2RAIN-ASCAT are evaluated to select appropriate blending SPDs in different climate regions. Six statistical indices, including mean bias (MB), mean absolute error (MAE), unbiased root mean square error (ubRMSE), correlation coefficient (R), Kling–Gupta efficiency (KGE), and Theil’s U coefficient, are used to assess the WALS-RBPD performance over 102 rain gauges (RGs) in Pakistan. The results showed that WALS-RBPD had assigned higher weights to IMERG in the glacial, humid, and arid regions, while SM2RAIN-ASCAT had higher weights across the hyper-arid region. The average weights of IMERG (SM2RAIN-ASCAT) are 29.03% (23.90%), 30.12% (24.19%), 31.30% (27.84%), and 27.65% (32.02%) across glacial, humid, arid, and hyper-arid regions, respectively. IMERG dominated monsoon and pre-monsoon seasons with average weights of 34.87% and 31.70%, while SM2RAIN-ASCAT depicted high performance during post-monsoon and winter seasons with average weights of 37.03% and 38.69%, respectively. Spatial scale evaluation of WALS-RPBD resulted in relatively poorer performance at high altitudes (glacial and humid regions), whereas better performance in plain areas (arid and hyper-arid regions). Moreover, temporal scale performance assessment depicted poorer performance during intense precipitation seasons (monsoon and pre-monsoon) as compared with post-monsoon and winter seasons. Skill scores are used to quantify the improvements of WALS-RBPD against previously developed blended precipitation datasets (BPDs) based on WALS (WALS-BPD), dynamic clustered Bayesian model averaging (DCBA-BPD), and dynamic Bayesian model averaging (DBMA-BPD). On the one hand, skill scores show relatively low improvements of WALS-RBPD against WALS-BPD, where maximum improvements are observed in glacial (humid) regions with skill scores of 29.89% (28.69%) in MAE, 27.25% (23.89%) in ubRMSE, and 24.37% (28.95%) in MB. On the other hand, the highest improvements are observed against DBMA-BPD with average improvements across glacial (humid) regions of 39.74% (36.93%), 38.27% (33.06%), and 39.16% (30.47%) in MB, MAE, and ubRMSE, respectively. It is recommended that the development of RBPDs can be a potential alternative for data-scarce regions and areas with complex topography. Full article

Staphylococcus aureus is a facultative pathogen that can encode numerous antibiotic resistance and immune evasion genes and can cause severe infections. Reduced susceptibility to last resort antibiotics such as vancomycin and daptomycin is often associated with mutations in walRK, an essential two-component regulatory system (TCS). This study focuses on the WalK accessory membrane proteins YycH and YycI and their influence on WalRK phosphorylation. Depletion of YycH and YycI by antisense RNA caused an impaired autolysis, indicating a positive regulatory function on WalK as has been previously described. Phosphorylation assays with full-length recombinant proteins in phospholipid liposomes showed that YycH and YycI stimulate WalK activity and that both regulatory proteins are needed for full activation of the WalK kinase. This was validated in vivo through examining the phosphorylation status of WalR using Phos-tag SDS-PAGE with a yycHI deletion mutant exhibiting reduced levels of phosphorylated WalR. In the yycHI knockdown strain, muropeptide composition of the cell wall was not affected, however, the wall teichoic acid content was increased. In conclusion, a direct modulation of WalRK phosphorylation activity by the accessory proteins YycH and YycI is reported both in vitro and in vivo. Taken together, our results show that YycH and YycI are important in the direct regulation of WalRK-dependent cell wall metabolism. Full article

Streamside native forests are known for their key role in water provision, commonly referred to as buffers that control the input or output of nutrients from terrestrial to aquatic ecosystems (i.e., nitrogen or carbon cycle). In order to assess the functional role of indigenous forests along streamside channels, we measured 10 parameters associated with DOM (Dissolved Organic Matter) at 42 points in 12 small catchments (15–200 ha) dominated by native forests (reference, WNF), forest plantations (WFP) and agricultural lands (WAL) in which the land cover portion was calculated in the entire watershed and along 30 and 60-m wide buffer strips. We found that watersheds WFP and WAL were statistically different than WNF, according to DIC concentrations (Dissolved Inorganic Carbon) and the intensity of the maximum fluorescence of DOM components. Using linear models, we related streamside native forest coverage in buffer strips with DOM parameters. The increase of streamside native forest coverage in 60 m wide buffer strips (0–100%) was related to lower DIC concentrations (0.89 to 0.28 mg C L⁻¹). In watersheds WFP and WAL, the humic and fulvic-like components (0.42 to 1.42 R.U./mg C L⁻¹) that predominated were related to an increase in streamside native forest coverage in the form of a 60 m wide buffer strip (0–75%). This is evidence that streamside native forests influence outputs of detritus and lowered in-stream processing with concomitant downstream transport, and functional integrity and water quality. We propose that DOM quantity and quality may be a potential tool for the identification of priority areas near streams for conservation and ecological restoration in terms of recovery of water quality as an important ecosystem service. The results of this study are useful to inform policy and regulations about the width of streamside native forests as well as their characteristics and restrictions. Full article