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Vet. Sci., Volume 6, Issue 3 (September 2019)
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Cover Story (view full-size image) Mycobacterial diseases, like bovine tuberculosis and paratuberculosis, are characterized by a long [...] Read more. Mycobacterial diseases, like bovine tuberculosis and paratuberculosis, are characterized by a long latent phase in which infected animals can be contagious but currently cannot be accurately diagnosed. To prevent the spread of mycobacterial infections, improved diagnostic assays are required that reliably detect infected animals in the pre-shedding phase. Transcriptomic methods offer a powerful approach to identify novel biomarkers, and these have been widely implemented to trace novel antigens with diagnostic potential. Further, some RNA species are promising direct diagnostic targets, as they are stably secreted into body fluids. One-step RNA detection by newly developed diagnostic platforms facilitates the future implementation of these tests in existing control programs. View this paper