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Open AccessArticle

Does Double Centrifugation Lead to Premature Platelet Aggregation and Decreased TGF-β1 Concentrations in Equine Platelet-Rich Plasma?

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Departamento de Clínica Médica, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo 05508-270, Brazil
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Departamento de Bioquímica, Escola Paulista de Medicina, UNIFESP, São Paulo 04023-062, Brazil
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Authors to whom correspondence should be addressed.
Vet. Sci. 2019, 6(3), 68; https://doi.org/10.3390/vetsci6030068
Received: 31 May 2019 / Revised: 29 July 2019 / Accepted: 31 July 2019 / Published: 21 August 2019
Blood-derived autologous products are frequently used in both human and equine medicine to treat musculoskeletal disorders. These products, especially the platelet-rich plasma (PRP), may contain high concentrations of growth factors (GFs), and thus improve healing in several tissues. Nevertheless, the procedures for preparation of PRP are currently non-standardized. Several protocols, which are based on distinct centrifugation patterns (rotation speed and time), result in PRPs with different characteristics, concerning platelet and GFs concentrations, as well as platelet activation. The aim of the present study was to compare two different protocols for PRP preparation: protocol (A) that is based on a single-centrifugation step; protocol (B), which included two sequential centrifugation steps (double-centrifugation). The results here reported show that the double-centrifugation protocol resulted in higher platelet concentration, while leukocytes were not concentrated by this procedure. Although platelet activation and aggregation were increased in this protocol in comparison to the single-centrifugation one, the TGF-β1 concentration was also higher. Pearson’s correlation coefficients gave a significant, positive correlation between the platelet counts and TGF-β1 concentration. In conclusion, although the double-centrifugation protocol caused premature platelet aggregation, it seems to be an effective method for preparation of PRP with high platelet and TGF-β1 concentrations. View Full-Text
Keywords: platelet-rich plasma; equine; platelet aggregation; growth factor platelet-rich plasma; equine; platelet aggregation; growth factor
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Seidel, S.R.T.; Vendruscolo, C.P.; Moreira, J.J.; Fülber, J.; Ottaiano, T.F.; Oliva, M.L.V.; Michelacci, Y.M.; Baccarin, R.Y.A. Does Double Centrifugation Lead to Premature Platelet Aggregation and Decreased TGF-β1 Concentrations in Equine Platelet-Rich Plasma? Vet. Sci. 2019, 6, 68.

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