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Correction published on 23 October 2019, see Vet. Sci. 2019, 6(4), 84.
Open AccessArticle

Detection of Bovine Leukemia Virus RNA in Blood Samples of Naturally Infected Dairy Cattle

1
Instituto de Virología, Instituto Nacional de Tecnología Agropecuaria (INTA), Buenos Aires C1686, Argentina
2
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Godoy Cruz 2290, Ciudad autónoma de Buenos Aires C1425FQB, Argentina
*
Author to whom correspondence should be addressed.
Vet. Sci. 2019, 6(3), 66; https://doi.org/10.3390/vetsci6030066
Received: 6 June 2019 / Revised: 9 July 2019 / Accepted: 16 July 2019 / Published: 6 August 2019
(This article belongs to the Section Microbiology and Immunology)
The viral expression in vivo, in bovine leukemia virus (BLV)-infected cattle, is considered to be restricted to extremely low levels, and the mitosis of infected B lymphocytes is regarded as the main mode of virus persistence within the infected host. In this study, the presence of BLV RNA in whole blood from seven asymptomatic cows naturally infected with BLV during one year, including a complete milking cycle and two delivery time points, was investigated by nested-PCR using the oligonucleotides complementary to the tax and pol gene. BLV RNA was detected in four cows at different time points, especially in high blood proviral load cows and around delivery time. This study describes the detection of free BLV RNA in blood from BLV-infected asymptomatic cows. The results obtained suggest the occurrence of persistent low-level expression of the tax and pol genes that could be a result of viral reactivation, within the asymptomatic period. This finding may be important in the pathogenesis of BLV infection, associated with the delivery period. View Full-Text
Keywords: bovine leukemia virus; RNA detection; viral expression; tax gene; pol gene bovine leukemia virus; RNA detection; viral expression; tax gene; pol gene
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MDPI and ACS Style

Alvarez, I.; Porta, N.G.; Trono, K. Detection of Bovine Leukemia Virus RNA in Blood Samples of Naturally Infected Dairy Cattle. Vet. Sci. 2019, 6, 66.

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