Oligosaccharides from human or bovine milk selectively stimulate growth or metabolism of bacteria associated with the lower gastrointestinal tract of infants. Results from complex infant-type co-cultures point toward a possible synergistic effect of combining bovine milk oligosaccharides (BMO) and lactose (LAC) on enhancing the metabolism of Bifidobacterium longum
and inhibition of Clostridium perfringens.
We examine the interaction between B. longum
and the commensal Parabacteroides distasonis
, by culturing them in mono- and co-culture with different carbohydrates available. To understand the interaction between BMO and lactose on B
and test the potential postbiotic effect on C. perfringens
growth and/or metabolic activity, we inoculated C. perfringens
into fresh media and compared the metabolic changes to C. perfringens
in cell-free supernatant from B. longum
fermented media. In co-culture, B. longum
benefits from P. distasonis
(commensalism), especially in a lactose-rich environment. Furthermore, B. longum
fermentation of BMO + LAC impaired C. perfringens
’ ability to utilize BMO as a carbon source (potential postbiotic effect).