Table of Contents
Metabolites, Volume 10, Issue 5 (May 2020) – 45 articles
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Cover Story (view full-size image) Bidens pilosa has many bioactivities, owing to its diverse phytochemicals. These include [...] Read more. Bidens pilosa has many bioactivities, owing to its diverse phytochemicals. These include chlorogenic acids, which occur as either regio- or geometrical isomers. To profile the CGA composition, extracts from tissues, callus, and cell suspensions were analyzed by UHPLC-qTOF-MS/MS. An ISCID method based on MS fragmentation capable of discriminating between closely related HCA derivatives of quinic acid was able to discriminate between positional isomers containing two different cinnamoyl moieties, such as a mixed di-ester of feruloyl-caffeoylquinic acid and coumaroyl-caffeoylquinic acid. Tissues and cell cultures of B. pilosa contained a combined total of 30 mono-, di-, and tri-substituted CGAs. In addition, tartaric acid esters as well as caftaric and chicoric acids were identified. Profiling revealed a differential distribution of these molecules across tissues types. View this paper<