Native and invasive plants of the riverain region undergo a range of environmental stresses that result in excess reactive oxygen species (ROS). Hydrogen peroxide (H
2O
2) is a relatively stable and quickly quantifiable way among different ROS. The herbaceous species
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Native and invasive plants of the riverain region undergo a range of environmental stresses that result in excess reactive oxygen species (ROS). Hydrogen peroxide (H
2O
2) is a relatively stable and quickly quantifiable way among different ROS. The herbaceous species including
Artemisia princeps,
Sicyos angulatus, and
Solidago altissima were selected. The H
2O
2 and photosynthetic pigment of leaves were measured, soil samples were analyzed to quantify macronutrients such as total nitrogen (TN), total phosphorus (TP), and soil moisture, and photosynthetic photon flux density (PPFD) was also recorded at different observed sites of Arakawa Tarouemon, Japan. The H
2O
2 concentration of
S. altissima significantly increased with high soil moisture content, whereas
A. Princeps and
S. angulatus significantly decreased with high soil moisture. In each species, H
2O
2 was negatively correlated with chlorophyll
a (chl
a) and chlorophyll b (chl
a). When comparing different parameters involving TN, TP, PPFD, and soil moisture content with H
2O
2 utilizing the general additive model (GAM), only soil moisture content is significantly correlated with H
2O
2. Hence, this study suggests that H
2O
2 would be an effective biomarker for quantifying environmental stress within a short time, which can be applied for riparian native and invasive plant species vegetation regulation.
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