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Sensors, Volume 19, Issue 22 (November-2 2019)
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Cover Story (view full-size image) We studied the photophysical behavior of a new luminogen. We confirmed the aggregation-induced [...] Read more. We studied the photophysical behavior of a new luminogen. We confirmed the aggregation-induced emission (AIE) phenomenon with different approaches by using different solvent, viscosity, and forming micelles. In all cases, we observed a strong enhancement of the fluorescence produced by reducing the intrarotational mobility of the dye. Finally, we studied the rate of spontaneous incorporation of the dye into cells and observed the intracellular pattern produced by AIE. Interestingly, different intracellular compartments present strong differences in fluorescence intensity and fluorescence lifetime. We used this difference to isolate different intracellular regions to allow us to selectively study these regions. Finally, we have demonstrated the use of this luminogen in bioimaging with a mitochondria biomarker to analyze these organelles through their fluorescence lifetimes. View this paper.