Water

The sustainable development of the water environment in the Yangtze River basin has become a critical issue in China. Turbidity is a comprehensive element for water quality monitoring. In this study, the baseline of water turbidity in the Yangtze River was constructed using 36 years of Landsat images from 1986 to 2021. The spatial and temporal dynamics of turbidity and its driving factors were explored. The results show that (i) the proposed Landsat-based turbidity model performs well, with a correlation coefficient (R²) of 0.68 and a Root Mean Square Error (RMSE) of 7.83 NTU for the whole basin. (ii) The turbidity level in the Yangtze River basin is spatially high in the upper reaches (41.7 NTU), low in the middle reaches (30.9 NTU), and higher in the lower reaches (37.6 NTU). The river turbidity level (60.1 NTU) is higher than the turbidity in lakes and reservoirs (29.6 NTU). The turbidity in the Yangtze River basin shows a decreasing trend from 1986 to 2021, with the most significant decrease in the mainstream of the Yangtze River. Seasonally, the mean turbidity in the Yangtze River basin shows a “low in summer and high in winter” trend, but opposite trends were revealed for the first time in rivers and lakes, such as Dongting Lake, Poyang Lake, and Taihu Lake, etc. (iii) Natural factors, including precipitation and natural vegetation cover (woodlands, grasslands, and shrubs) could explain 58% of the turbidity variations, while human activities including impervious surfaces, cropland, and barren land are lower impact. Annual precipitation was negatively correlated with water turbidity, while cropland and barren land showed a significant positive correlation. The study is of great practical value for the sustainable development of the water environment in the Yangtze River basin and provides a reference for remote sensing monitoring of the water environment in inland water bodies. Full article

Serious soil and water loss affects the economy and the living quality of the population in faulted basins. Since 2002, China has carried out large-scale ecological restoration projects in karst areas. However, the karst faulted basins have experienced complex climatic changes, which makes it difficult to assess the ecological restoration effects quantitatively. Using the improved Budyko model, basin factor (n) and sediment content factor (C) were divided into climate influence and ecological restoration influence by second-order approximation, and the change ratio of climate influence and ecological restoration on sediment yield and loss was quantitatively calculated in the representative basin of the Nandong Underground River System (NURS). This was carried out in order to effectively distinguish the effects of climate change and ecological restoration on runoff and sediment change in the NURS. Furthermore, this study sought to understand the mechanism of runoff and sediment change and to evaluate the ecological restoration in the faulted basin to provide a scientific basis for the next stage of soil and water conservation policy formulation. The results showed that: (1) Using Pettitt to test the abrupt change of water and sediment in the NURS from 1987 to 2018, we found that 2002 was the abrupt change year of water and sediment in the basin. Before and after the mutation, the runoff and sediment yield decreased significantly, with the change rates of −15.5% and −51.8%, respectively. The decrease in precipitation and the increase in E₀ were not significant, which were −4.5% and 1.4% respectively. (2) By calculating the correlation coefficient and the double logarithm function, it was found that the maximum temperature is the main climatic factor affecting the underlying surface factor n, and precipitation is the main climatic factor affecting the sediment concentration c. (3) Climate change reduced runoff by 77.6%. Ecological restoration has reduced sediment by 51.3 percent. The sensitivity of runoff to maximum temperature was 3.61. The sensitivity of grain yield to precipitation and NDVI was 5.37 and 3.26, respectively. The results show that climate is the main factor of runoff reduction, and ecological restoration is the main factor of sediment reduction. Ecological restoration has greatly reduced sediment production, and ecological engineering has made remarkable progress. However, the reduction of runoff caused by climate change should be paid more attention. The results of these studies will help to better formulate land use management policies for soil and water conservation. Full article

In a recirculating aquaculture system (RAS), feed is critical to the growth of fish and is the main source of nutrient pollutants in aquaculture water. An eight-week feeding trial was conducted to investigate the role of feed on the growth efficiency of hybrid grouper (Epinephelus lanceolatus ♂ × Epinephelus fuscoguttatus ♀) and water quality in a RAS. Five commercial feeds with different respective dietary protein/energy (P/E) ratios and available phosphorus levels were selected (LNLP, 31.97 g/MJ, 0.96%; LNMP, 32.11 g/MJ, 1.54%; MNLP, 36.26 g/MJ, 0.98%; MNMP, 36.53 g/MJ, 1.58%; and HNP, 41.54 g/MJ, 1.97%). The results showed that HNP had the highest growth efficiency and MNLP provided the best economic benefit. The trend in water quality within 6 h after feeding was similar among the five groups. The relative concentrations of ammonia nitrogen, total nitrogen, active phosphate, and total phosphorus reached a maximum 2 h after feeding, and the relative concentration of nitrite reached a maximum 1 h after feeding. The high P/E ratio feed increased the concentrations of total ammonia nitrogen and nitrite nitrogen. The total ammonia nitrogen concentration in HNP was much higher than those in the other treatments. The dietary P/E ratio had no significant effect on total nitrogen concentration. High dietary phosphorus levels increased the total phosphorus concentration in the water, but no significant effect on the active phosphate concentration was observed. Considering the growth efficiency, economic benefit, and water quality, it can be concluded that MNLP is the most suitable feed for RAS breeding hybrid grouper. The results of this study supplement the gap on the effects of feed on RAS water quality and provide data support for the sustainable development of RAS industry. Full article

Atrazine (ATZ) is an herbicide used for increased food production due to its weed and pesticide control capacity in different crops. However, ATZ is a chemical compound that is harmful to the environment and human health, and, unfortunately, it has been detected in surface and groundwater. Therefore, the aim of this paper was to perform the adsorption of Atrazine from a synthetically contaminated water sample using a packed-bed column with a low-cost adsorbent prepared from Moringa oleifera Lam. seeds. The synthesized adsorbent presented an increase in the surface specific area (S_BET) of 37% in comparison with the in natura material. The effects of the peristaltic pump flow rate (Q), concentration of the ATZ inlet ([ATZ]_inlet) solution, and bed height (H) were studied, with the highest percentage of ATZ removed through the adsorption column (50, 0%) obtained with a packed-bed column with H = 13 cm bed height, Q = 1 mL/min, [ATZ]_inlet = 2.0 mg/L, pH = 5.0, a breakthrough time of 25 min, and a saturation time of 420 min. The logistic model was used to best fit the experimental data with an R² > 0.99, and the Bohart–Adams, Thomas, and Yoon–Nelson models were used to explain and analyze the obtained effects in the continuous adsorption of ATZ. Therefore, the Moringa oleifera Lam. seeds provided a low-cost adsorbent for the continuous adsorption of the herbicide Atrazine in a packed-bed column. Full article

The purpose of this work is to evaluate the applicability of five drought indices (Surface Water Supply Index—SWSI, Reclamation Drought Index—RDI, Streamflow Drought Index—SDI, Standardized Precipitation Index—SPI, and Evaporative Demand Drought Index—EDDI) as tools for monitoring, in identifying the duration, intensity, and frequency of hydrological droughts in the basins of the Banabuiú, Castanhão, and Orós reservoirs, located in the state of Ceará, Brazil. The analysis focused on determining the performance of the indexes for capturing the droughts characteristics from 2002 to 2020. Thus, the comparison of the values of the indexes with the Target Levels of the reservoir’s operation were used, as well as the analysis of six decision criteria: robustness, tractability, transparency, sophistication, extensibility, and dimensionality, to compare the behavior of drought indices. The results of the evaluation criteria showed that the SPI was superior to the other indices, being able to significantly represent the drought episodes, capturing a greater number of events. Thus, SPI received the highest total weighted score (118), followed by SWSI (97), EDDI (95), SDI (95), and RDI (88). In this context, it was found that the SPI and SWSI are the most suitable indices to monitor drought in the region and that the use of longer time scales can be recommended to manage hydrological droughts, in order to improve planning and management of water resources. Full article

As a passive technology, constructed wetlands (CWs) are promising candidates for mine-drainage treatment. However, the design and operation of CWs have not been fully established because the chemical compositions of mine drainage are diverse. In this study data sets of 100 mine drainages in Japan were classified using multivariate analysis based on water quality. Mine drainage was classified into eight types based on the ratio of the concentrations of Cd, Pb, As, Cu, Zn, Fe, and Mn to the effluent standard: (I) neutral and low metal concentration, (II) weakly acidic and low metal concentration, (III) weakly acidic and high Zn concentration, (IV) weakly acidic and high Mn and Zn concentrations, (V) acidic and high As concentration, (VI) acidic and high Fe concentration, (VII) acidic and extremely high Fe concentration, and (VIII) acidic and high Zn concentration. Mechanisms for removing metals in CWs were discussed based on this classification. Metal hydroxides of Cu, Pb, Zn, and Cd can precipitate with an increasing pH. Under oxidative conditions, dissolved Fe and Mn are oxidized to metal oxides. Under reductive conditions, Pb, Zn, Cd, and Cu precipitate as metal sulfides. This classification of mine drainage will be helpful in the systematic design and operation of CWs. Full article

UV-LED irradiation has attracted attention in water and wastewater disinfection applications. However, no studies have quantitatively investigated the impact of light intensity on the UV dosage for the same magnitude of disinfection. This study presents a powerful 280 nm UV-LED photoreactor with adjustable light intensity to disinfect municipal wastewater contaminated with E. coli, SARS-CoV-2 genetic materials and others. The disinfection performance of the 280 nm LED was also compared with 405 nm visible light LEDs, in terms of inactivating E. coli and total coliforms, as well as reducing cATP activities. The results showed that the UV dose needed per log reduction of E. coli and total coliforms, as well as cATP, could be decreased by increasing the light intensity within the investigated range (0–9640 µW/cm²). Higher energy consumption is needed for microbial disinfection using the 405 nm LED when compared to 280 nm LED. The signal of SARS-CoV-2 genetic material in wastewater and the SARS-CoV-2 spike protein in pure water decreased upon 280 nm UV irradiation. Full article

The effect of low temperature on microbial nitrogen metabolism in constructed wetlands has yet to be extensively investigated. In this study, we analyzed the effects of temperature changes on nitrogen-associated microorganisms and nitrogen metabolism functional genes in a multi-stage surface flow constructed wetland (MSSFCW) using metagenomic sequencing. The treatment of polluted river water in the MSSFCW, which had a mean water temperature (MWT) of ≤17 °C, resulted in a low removal efficiency (RE) for total nitrogen (TN; average RE: 23.05% at 1–17 °C) and nitrate nitrogen (NO₃⁻-N; average RE: −2.41% at 1–17 °C). Furthermore, at a MWT of ≤11 °C, the REs were low for ammonium nitrogen (NH₄⁺-N; average RE: 67.92% at 1–11 °C) and for chemical oxygen demand (COD; average RE: 27.45% at 1–11 °C). At 0.24 m³ m⁻² d⁻¹ influent load, the highest REs for TN (66.84%), NO₃⁻-N (74.90%), NH₄⁺-N (83.93%), and COD (52.97%) occurred in July and August, when water temperatures were between 26 and 28 °C. The lowest rates (TN: 11.90%, NO₃⁻-N: −21.98%, NH₄⁺-N: 65.47%, COD: 24.14%) occurred in the January–February period, when the water temperature was lowest (1–5 °C). A total of 25 significantly different species were detected in surface sediment, none of which were dominant species. The dominant phyla and genera at low (January) and high (July) temperatures were similar; however, microorganisms were more abundant in the low-temperature months. Our analysis indicated that the same nitrogen metabolism pathways occurred in January and July. Denitrification-associated functional genes were the most abundant; nitrification-related functional genes were the least abundant. Only nirBD displayed significantly different abundances between January and July. This paper can hopefully help researchers and managers further understand how temperature affects nitrogen removal performance in constructed wetlands. Full article

Phytoplankton communities, showing significant spatiotemporal variation within bay areas, play an important role in the structure and function of nearshore marine ecosystems. However, the absence of long-term high-resolution datasets has hindered our understanding of the effect of ENSO-driven environmental changes on phytoplankton communities in coastal ecosystems. Herein, by performing biomarker inversion analyses on two centuries’ worth of sedimentary organisms in the Sanmen Bay area, we observed a marked El Niño/La Niña-related succession; specifically, that El Niño-induced warming had increased the biomass of phytoplankton by 57.89%, while also increasing the proportion of diatoms by 76.40%. In contrast, La Niña years exhibited a decrease in the biomass of phytoplankton by 54.23%. Further, over three decades of observational data from the Sanmen Bay suggest that La Niña years can promote occasional blooms through monsoonal mixing and land-based inputs. Consequently, the nearshore marine ecosystem of the bay area, being subject to intense anthropogenic activity and land–sea interactions, can be said to be influenced by global-scale ocean–atmosphere processes. Going forward, the connection between short-term extreme events and long-term changes in the nearshore marine ecosystem should receive greater attention. Full article

Metals stored in sediments of lakes can bioaccumulate through the food chain, posing a risk to the environment and human health. Alpine lakes are supposed to be less affected by pollution than lowland lakes and are vulnerable to any changes and impacts in their catchment areas because of their remote position and ultra-oligotrophic character. Therefore, we used a model Alpine lake, Bohinj (in the Triglav National Park, Julian Alps, Slovenia), to evaluate the load of metals in the abiotic and biotic compartments of the ecosystem, in order to assess the spatial distribution of metals, and finally, to determine whether past and present human activities in the lake’s catchment area may be causing pollution. To this aim, the contents of Cu, Pb, Cr, Cd, Co, Mn, Fe, Zn, Hg and Ni in the sediment, water, and macrophyte samples were determined. The results showed that the average content of some toxic elements, especially in the sediments (Cd 0.52 mg/kg; Hg 0.03 mg/kg) and plants (Co 0.71 mg/kg; Cr 5.88 mg/kg) was elevated compared to natural background values. High Hg contents could be connected with natural geological sources, while other elements were probably of anthropogenic origin. High levels of all elements in the eastern part of the lake indicated long-term pollution, which could be a consequence of past iron extraction and military activities in the vicinity. On the other hand, high contents of elements in the water suggests that intensive touristic activities in the area may cause temporal pollution in the summer. The study sheds light on complicated processes governing the distribution of trace metals in Alpine lakes. Full article

The study ascertained the relationship between aquaculture production and greenhouse gas (GHG) emissions in South Africa. The study used the Autoregressive Distributed Lag—Error Correction Model (ARDL-VECM) with time series data between 1990 and 2020. The results showed that the mean annual aquaculture production, GHG emissions, and Gross Domestic Product (GDP) in the period were 5200 tonnes, 412 tonnes, and US$447 billion, respectively. There was a long-run relationship between GHG emissions and GDP. In the short run, GHG emissions had a positive relationship with GDP and a negative relationship with beef production. Furthermore, there was a bi-directional relationship between aquaculture production and GHG emissions. In addition, beef production and GDP had a bi-directional relationship. Beef production also had a positive relationship with aquaculture production. The study concludes that aquaculture production is affected and tends to affect GHG emissions. Aquaculture legislation should consider GHG emissions in South Africa and promote sustainable production techniques. Full article

The temporal distribution of trace elements in a sediment core (SK117/GC-08) indicates minor changes in oxygenation during the last 100 kilo years in the bottom waters of the deeper eastern Arabian Sea. The high values of Mn, Co, Cu, Mn/Al, Co/Al, Cu/Al, V/Cr, and V/(V + Ni) in the sediments during interglacials and interstitials collectively indicate oxic conditions during warm periods. The high values of Cr, Ni, V, Mo, Cr/Al, Ni/Al, and Ni/Co in sediments during glacials and stadials collectively indicate dysoxic to suboxic conditions during the colder last glacial maximum and during the entire marine isotope stage two. The bottom waters have never experienced anoxic conditions. Multivariate statistics showed the attribution of the trace elements in two factors: cluster 1 (Co, Cu, Mn) was enriched during oxic conditions and cluster 2 (Cr, Mo, Ni, V) was enriched during dysoxic to suboxic conditions. Oxygenation conditions are mainly driven by variations in monsoon-controlled surface water productivity and changes in the flux of circumpolar deep water. The dysoxic to suboxic bottom water conditions developed at the core location during colder climates are very well synchronised with an increased organic matter flux. The main factor that controls the accumulation of the organic-rich sediments in the eastern Arabian Sea during a glacial is the increase in the supply of organic matter from increased primary productivity in the surface waters, controlled by winter monsoon winds, and localized convective mixing. During warmer interglacials and interstadials, the core location has remained well ventilated. Full article

Coastal zones are ecosystems that are sensitive to climate change and anthropogenic pollution, resulting in a potential loss of biodiversity and ecosystem services through eutrophication and nutrient imbalances, among others. The coastal El Sauce catchment area, Central Chile, is under multiple anthropogenic pressures including wastewater treatment plant (WWTP) discharge, which its broad effect remains underexplored. In order to assess the impact of the WWTP on El Sauce stream, the benthic microbial communities and key functional groups variability (i.e., nitrifiers, methanogens and methanotrophs) were determined by 16S rDNA high-throughput sequencing and by functional genes quantification, respectively, during two contrasted seasons in three catchment areas (pre-, WWTP and post-discharge). The microbial communities’ structure profiles were associated with the water quality, nutrients, greenhouse gas (GHG) distribution, and the organic matter isotopic signatures in the sediments, for the first time, in this ecosystem. The results show that organic matter isotopic signatures using nitrogen and carbon (δ¹⁵N and δ¹³C) and the physicochemical conditions in El Sauce estuary changed from the pre- to WWTP discharge areas (i.e., a pH decrease of 0.5 units and an increase of 4–6 °C in the water temperature). The WWTP discharge area was characterized by a low nutrient concentration and significantly higher GHG distribution (>600 µM CO₂, >30,000 nM CH₄, and >3000 nM N₂O). In addition, the benthic microbial community structure shifted spatially and seasonally, including specific phyla known as sewage bioindicators, such as Firmicutes (Clostridiales order) and Bacteroidetes. In addition, other taxa were enriched or only retrieved in the sediments of the WWTP influenced area, e.g., Tenericutes, Lentisphaerae, Synergistetes, and LCP-89. Methanogens were more enriched near the WWTP discharge compared to those in the pre-discharge site in both seasons, while methanotrophs and ammonia oxidizers were unfavored only during winter. Our results indicate that the WWTP discharge impacts the biogeochemical conditions in El Sauce catchment area modifying the benthic microbial communities, including a decrease in the key functional groups able to mitigate CH₄ and regulate nutrients recycling in these aquatic ecosystems. Full article

Quantitatively analyzing models’ uncertainty is essential for agricultural models due to the effect of inputs parameters and processes on increasing models’ uncertainties. The main aim of the current study was to explore the influence of input parameter uncertainty on the output of the well-known surface irrigation software model of WinSRFR. The generalized likelihood uncertainty estimation (GLUE) framework was used to explicitly evaluate the uncertainty model of WinSRFR. The epistemic uncertainties of WinSRFR furrow irrigation simulations, including the advance front curve, flow depth hydrograph, and runoff hydrograph, were assessed in response to change key input parameters related to the Kostiakov–Lewis infiltration function, Manning’s roughness coefficient, and the geometry cross section. Three likelihood measures of Nash–Sutcliffe efficiency (NSE), percentage bias (PBIAS), and the coefficient of determination (R²) were used in GLUE analysis for selecting behavioral estimations of the model outputs. The uncertainty of the WinSRFR model was investigated under two furrow irrigation system conditions, closed end and open end. The results showed the likelihood measures considerably influence the width of uncertainty bounds. WinSRFR outputs have high uncertainty for cross section parameters relative to soil infiltration and roughness parameters. Parameters of soil infiltration and roughness coefficient play an important role in reducing the uncertainty bound width and number of observations, especially by filtering non-behavioral data using likelihood measures. The simulation errors of advance front curve and runoff hydrograph outputs with a PBIAS function were relatively lower and stable compared with other those of the likelihood functions. The 95% prediction uncertainties (95PPU) of the advance front curve were calculated to be 87.5% in both close-ended and open-ended conditions whereas, it was 91.18% for the runoff hydrograph in the open-ended condition. Additionally, the NSE likelihood function more explicitly determined the uncertainty related to flow depth hydrograph estimations. The outputs of the model showed more uncertainty and instability in response to variability in soil infiltration parameters than the roughness coefficient did. Therefore, applying accurate field methods and equipment and proper measurements of soil infiltration is recommended. The results highlight the importance of accurately monitoring and determining model input parameters to access a suitable level of WinSRFR uncertainty. In conclusion, considering and analyzing the uncertainty of input parameters of WinSRFR models is critical and could provide a reference to obtain realistic and stable furrow irrigation simulations. Full article

Leachate generation is among the main challenging issues that landfill operators must handle. Leachate is created when decomposed materials and rainwater pass through the waste. Leachate carries many harmful pollutants, with high concentrations of BOD, COD, colour, heavy metals, ammoniacal nitrogen (NH₃-N), and other organic and inorganic pollutants. Among them, COD, colour, and NH₃-N are difficult to be completely eliminated, especially with a single treatment. They should be handled by appropriate treatment facilities before being safely released into the environment. Leachate remediation varies based on its properties, the costs of operation and capital expenditures, as well as the rules and regulations. Up until now, much scientific and engineering attention was given to the development of comprehensive solutions to leachate-related issues. The solutions normally demand a multi-stage treatment, commonly in the form of biological, chemical, and physical sequences. This review paper discussed the use of contemporary techniques to remediate landfill leachate with an emphasis on concentrated COD, colour, and NH₃-N levels with low biodegradability that is normally present in old landfill or dumping grounds in developing countries. A semi-aerobic type of landfill design was also discussed, as this concept is potentially sustainable compared to others. Some of the challenges and future prospects were also recommended, especially for the case of Malaysia. This may represent landfills or dumpsites in other developing countries with the same characteristics. Full article

Lateral subsurface flow (LSF) is a phenomenon that is widely occurring including the hummocky ground moraine landscape. Due to the heterogeneous structure of the subsurface, transport times of pesticides and nutrients from agricultural areas to adjacent water bodies are difficult to assess. Here, LSF at Luvisol and Regosol plots of an experimental field were studied by applying potassium bromide along a 10 m trench below the plow pan in October 2019. The soil solution was collected in suction cups 3 m downslope of the trench and in April 2021, the soil was sampled down to 1 m depth. Almost no bromide was found in the soil solution except for the 160 cm depth of the Regosol plot after a 541 day period. After the same time, bromide was observed in the 90 cm soil depth directly underneath the application trench of the Luvisol plot. A 3D reconstruction of the subsurface horizon boundaries of the Regosol revealed subsurface heterogeneities such as sand lenses that might have been attributed to the heterogeneous subsurface flow pattern. Full article

Non-point sources of water pollution caused by agricultural crop production are a serious problem in Czechia, at present. This paper describes a new approach for the mutual delineation and assessment of different pollution sources where the critical points method is used to identify the origin of contamination and the source areas. The critical points, i.e., sites presenting the entry of quick surface and drainage runoff into waters, are classified into three (for surface pollution sources using a WaTEM/SEDEM model) or four (subsurface = drainage sources via the catchment-measures need index) categories, respectively. This enabled us to prioritize the most endangered areas at different scales, ranging from the third-order catchments to very small subcatchments, and to design the appropriate combination of control measures to mitigate surface and drainage water runoff, with these being the main drivers of associated pollution. This methodology was applied to a study conducted in the Czech Republic within the entire Vltava River basin, with a total area of 27,578 km², and utilized in depth to assess a 543 km² catchment of the Vlašimská Blanice River. When the effect of the designed surface runoff control measures system had been assessed for sediment transport through outlet profiles of the fourth-order catchments, the average reduction reached 43%. The total reduction in the subsurface transport of nitrogen within the fourth-order catchments was 24%. The approach and results are planned to be projected into river basin management plans for the Vltava River basin. Nevertheless, a thorough reassessment of current legislations and strategies is needed to enable the broader adoption of mitigation measures and sustainable management patterns within agricultural landscapes. Full article

The study of gene diversity in interspecies contributes to our understanding of the environmental adaptation, evolutionary history, origin, and stability of biodiversity. Crabs are the main component of the macrobenthos community; therefore, how crabs adapt to different environments can be a guide to understand how to maintain community diversity. Previous studies on environmental adaptation have focused on differences in morphology, organ structure, and function, but there is a lack of research that explores this topic from the perspective of gene diversity in benthonic crabs. In this study, the leucosiid crab was selected for transcriptome assembly and was analyzed as this superfamily is one of the main clades in brachyuran crabs. Their transcriptome data were used to understand the gene diversity, phylogeny, and divergence time estimations. Subsequently, candidate gene families for depth adaptation were found in eight species that live in habitats with different depths. The results indicated that the number of total unigene sequences was between 65,617 (Philyra malefactrix) and 98,279 (Arcania heptacantha) in eight species. The total length of the genes was counted to be between 48,006,211 and 95,471,088 bp. The age of the superfamily Leucosioidea is over 150 Ma, dating back to at least the Jurassic geological period. The divergence of the family Leucosiidae would have occurred in the middle Cretaceous (around 100 Ma). After dividing into groups of three depth types, which carried the gene families, it was found that the three groups shared the solute carrier family, whereas bile acid secretion, organic solute transporter subunit alpha-like, and solute carrier organic anion transporter families only existed in the shallow group. This result shown that the gene function of ion concentration regulation might one of the candidate gene families related to the environmental adaptation of the leucosiid crab. Hence, these gene families will be analyzed in future studies to understand the mechanism of depth adaptation regulation in crabs. Full article