Special Issue "Advances in Foodborne Pathogen Analysis"

A special issue of Foods (ISSN 2304-8158). This special issue belongs to the section "Food Microbiology".

Deadline for manuscript submissions: 10 February 2020.

Special Issue Editors

Prof. Dr. Arun K. Bhunia
E-Mail Website
Guest Editor
Molecular Food Microbiology Laboratory, Department of Food Science, Department of Comparative Pathobiology (Courtesy), Purdue University, West Lafayette, IN, USA
Tel. +1 (765)-494-5443
Interests: microbiology; pathogenesis; host–pathogen interaction; nanobiotechnology; food safety
Special Issues and Collections in MDPI journals
Prof. Bledar Bisha
E-Mail Website
Guest Editor
Department of Animal Science, University of Wyoming, Laramie, WY, USA
Interests: food microbiology and microbial safety of foods; ecology of foodborne pathogens; rapid diagnostics; sample preparation; study of microorganisms at the single cell level; antimicrobial resistance; microbial source tracking
Special Issues and Collections in MDPI journals
Dr. Andrew G. Gehring
E-Mail Website
Guest Editor
Molecular Characterization of Foodborne Pathogens, Agricultural Research Service, United States Department of Agriculture, Wyndmoor, PA, USA
Interests: food safety; rapid methods; biosensors; immunoassays; sample preparation; multiplexed detection and identification of zero tolerance foodborne pathogens
Prof. Dr. Byron F. Brehm-Stecher
E-Mail Website
Guest Editor
Department of Food Science and Human Nutrition, Iowa State University, Ames, IA, USA
Interests: rapid detection of pathogens; pre-analytical sample preparation; flow cytometry and other methods for single cell analysis; multicomponent antimicrobial systems for use in foods, on food contact surfaces or in environmental applications; leveraging advances in materials science and chemistry for detection or inactivation of pathogens

Special Issue Information

Dear Colleagues,

Traditional enrichment culture combined with differential and selective plating serve as gold standards for the detection and identification of foodborne bacterial pathogens. Although accurate and sensitive, culture-based methods are labor intensive and time consuming with the results often taking from days to weeks. There is a desire to replace these methods with “rapid methods” that typically employ highly sensitive and fast biosensor-based analysis. However, creative thought and/or diligent empirical effort are required to introduce paradigm shifts in the generation of rapid methods capable of replacing culture-based methods if the need for comparable sensitivity and accuracy is warranted.  Therefore, this Special Issue will showcase contributions ranging from novel sample preparative techniques (like advances in filtration, centrifugation, chemical or particle-based separation, etc.) for the selective, rapid, efficient, and/or quantitative recovery and concentration of targeted bacterial analytes to the development and/or improvement of rapid biosensor-based detection and/or identification platforms (e.g., PCR, phage, immunoassays, optical imaging, luminescence, strip assays, flow cytometry, sequencing, etc.). A further challenge is the determination of live vs. dead organisms. Advances in this field are expected to impact the food production/testing industry and regulatory agencies alike.

Original and review papers dealing with all aspects of “Advances in Foodborne Pathogen Analysis” are welcome for inclusion in this Special Issue of Foods. Reports will focus on the following areas: development and/or improvement of rapid methods for the detection and/or identification of foodborne pathogens (methods, reagents, instrumentation); sample preparation techniques for the rapid and quantitative concentration of targeted microbes in food matrices; new approaches for determining or characterizing pathogen activity, metabolic state or viability.

Prof. Dr. Arun K. Bhunia
Prof. Bledar Bisha
Dr. Andrew Gehring
Prof. Dr. Byron F. Brehm-Stecher
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Foods is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.


  • food safety
  • food pathogens
  • sample preparation
  • biosensors
  • rapid methods
  • detection
  • identification
  • food biosecurity

Published Papers (1 paper)

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Open AccessArticle
Optimization of a Microplate Assay for Generating Listeria Monocytogenes, E. Coli O157:H7, and Salmonella Biofilms and Enzymatic Recovery for Enumeration
Foods 2019, 8(11), 541; https://doi.org/10.3390/foods8110541 - 02 Nov 2019
Biofilms enable the persistence of pathogens in food processing environments. Sanitizing agents are needed that are effective against pathogens entrapped in biofilms that are more difficult to inactivate than planktonic cells that are displaced and found on equipment surfaces. We examined conditions to [...] Read more.
Biofilms enable the persistence of pathogens in food processing environments. Sanitizing agents are needed that are effective against pathogens entrapped in biofilms that are more difficult to inactivate than planktonic cells that are displaced and found on equipment surfaces. We examined conditions to develop, analyze, and enumerate the enhanced biofilms of three different foodborne pathogens assisted by fluorescence adherence assay and enzymatic detachment. We compared three different isomeric forms of fluorescent substrates that are readily taken up by bacterial cells based on carboxy-fluorescein diacetate (5-CFDA, 5,6-CFDA, 5,6-CFDA, SE). Biofilm-forming strains of Escherichia coli O157:H7 F4546 and Salmonella Montevideo FSIS 051 were identified using a microplate fluorescence assay defined previously for L. monocytogenes. Adherence levels were determined by differences in relative fluorescence units (RFU) as well as recovered bacterial cells. Multiple hydrolytic enzymes were examined for each representative pathogen for the most suitable enzyme for detachment and enumeration to confirm adherence data obtained by fluorescence assay. Cultures were grown overnight in microplates, incubated, washed and replenished with fresh sterile growth medium; this cycle was repeated for seven consecutive days to enrich for robust biofilms. Treatments were performed in triplicate and compared by one-way analysis of variance (ANOVA) to determine significant differences (p < 0.05). Full article
(This article belongs to the Special Issue Advances in Foodborne Pathogen Analysis)
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