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Article

Multiplex Recombinase Polymerase Amplification Assay for the Simultaneous Detection of Three Foodborne Pathogens in Seafood

1
Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, China Jiliang University, Hangzhou 310018, China
2
Department of Plant Biology, Uppsala BioCenter, Linnean Centre for Plant Biology, Swedish University of Agricultural Science (SLU), SE-75007 Uppsala, Sweden
*
Author to whom correspondence should be addressed.
Foods 2020, 9(3), 278; https://doi.org/10.3390/foods9030278
Received: 17 February 2020 / Revised: 23 February 2020 / Accepted: 25 February 2020 / Published: 3 March 2020
(This article belongs to the Special Issue Advances in Foodborne Pathogen Analysis)
Foodborne pathogens can cause foodborne illness. In reality, one food sample may carry more than one pathogen. A rapid, sensitive, and multiple target method for bacteria detection is crucial in food safety. For the simultaneous detection of Staphylococcus aureus, Vibrio parahaemolyticus, and Salmonella Enteritidis, multi-objective recombinase polymerase amplification (RPA) combined with a lateral flow dipstick (LFD) was developed in this study. The whole process, including amplification and reading, can be completed in 15 min at 37 °C. The detection limits were 2.6 × 101 CFU/mL for Staphylococcus aureus, 7.6 × 101 CFU/mL for Vibrio parahaemolyticus, and 1.29 × 101 CFU/mL for Salmonella Enteritidis. Moreover, colored signal intensities on test lines were measured by a test strip reader to achieve quantitative detection for Staphylococcus aureus (R2 = 0.9903), Vibrio parahaemolyticus (R2 = 0.9928), and Salmonella Enteritidis (R2 = 0.9945). In addition, the method demonstrated good recoveries (92.00%–107.95%) in the testing of spiked food samples. Therefore, the multiplex LFD-RPA assay is a feasible method for the rapid, sensitive, and quantitative detection of bacterial pathogens in seafood. View Full-Text
Keywords: recombinase polymerase amplification; lateral flow dipstick; multiplex detection; quantitative detection; Staphylococcus aureus; Vibrio parahaemolyticus; Salmonella Enteritidis recombinase polymerase amplification; lateral flow dipstick; multiplex detection; quantitative detection; Staphylococcus aureus; Vibrio parahaemolyticus; Salmonella Enteritidis
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MDPI and ACS Style

Ma, B.; Li, J.; Chen, K.; Yu, X.; Sun, C.; Zhang, M. Multiplex Recombinase Polymerase Amplification Assay for the Simultaneous Detection of Three Foodborne Pathogens in Seafood. Foods 2020, 9, 278. https://doi.org/10.3390/foods9030278

AMA Style

Ma B, Li J, Chen K, Yu X, Sun C, Zhang M. Multiplex Recombinase Polymerase Amplification Assay for the Simultaneous Detection of Three Foodborne Pathogens in Seafood. Foods. 2020; 9(3):278. https://doi.org/10.3390/foods9030278

Chicago/Turabian Style

Ma, Biao, Jiali Li, Kai Chen, Xiaoping Yu, Chuanxin Sun, and Mingzhou Zhang. 2020. "Multiplex Recombinase Polymerase Amplification Assay for the Simultaneous Detection of Three Foodborne Pathogens in Seafood" Foods 9, no. 3: 278. https://doi.org/10.3390/foods9030278

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