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Open AccessArticle

A Molecular Tool for Rapid Detection and Traceability of Cyclospora cayetanensis in Fresh Berries and Berry Farm Soils

1
Facultad de Química, Universidad Autónoma de Querétaro, Cerro de las Campanas S/N, Querétaro 76010, Mexico
2
Hospital Infantil de Morelia Eva Samano de López Mateos, Servicio de Salud de Michoacán, Interior Bosque Cuauhtémoc S/N, Michoacán 58020, Mexico
3
Posgrado en Recursos Genéticos y Productividad, Producción de Semillas, Colegio de Postgraduados, Km. 36.5 Carretera Mexico-Texcoco, Estado de Mexico 56230, Mexico
*
Author to whom correspondence should be addressed.
Foods 2020, 9(3), 261; https://doi.org/10.3390/foods9030261
Received: 29 January 2020 / Revised: 16 February 2020 / Accepted: 23 February 2020 / Published: 2 March 2020
(This article belongs to the Special Issue Advances in Foodborne Pathogen Analysis)
Due to recent outbreaks of cyclosporiasis associated with consumption of fresh berries, producers are demanding modern microbiological tools for the rapid and accurate identification of the human pathogen Cyclospora cayetanensis in berries and environmental samples. The aim of the present work was to develop a molecular tool based on a PCR approach for the rapid and accurate detection of C. cayetanensis. A nested PCR assay was validated for the amplification of a 294 bp size region of the 18S rRNA gene from C. cayetanensis. The limit of detection for the nested PCR assay was validated using 48 berry samples spiked with ~0, 10, 100, and 1000 oocyst per gram of sample. With this assay, it was possible to detect as few as 1 oocyst per gram of berry, in a 50 g sample. Sanger DNA sequencing and phylogenetic analysis were carried out to confirm the presence of C. cayetanensis in berry (n = 17) and soil (n = 5) samples. The phylogenetic analysis revealed that the C. cayetanensis sequences obtained from Mexico clustered within a group recovered from China, Peru, Guatemala-Haiti, and Japan. The PCR protocol designed in the present study could be an important tool for the rapid and accurate detection of this human pathogen in environmental and food samples. View Full-Text
Keywords: Cyclospora cayetanensis; PCR; Mexico; berries; soil Cyclospora cayetanensis; PCR; Mexico; berries; soil
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Resendiz-Nava, C.N.; Orozco-Mosqueda, G.E.; Mercado-Silva, E.M.; Flores-Robles, S.; Silva-Rojas, H.V.; Nava, G.M. A Molecular Tool for Rapid Detection and Traceability of Cyclospora cayetanensis in Fresh Berries and Berry Farm Soils. Foods 2020, 9, 261.

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