Multiplexed Imaging for Cancer Diagnosis and Therapy: Dissecting Networks at Tissue or Single-Cell Level

Dear Colleagues,

The need to co-localize several markers at tissue or cellular level has a long history. Starting from the early use of serial tissue sections in white field microscopy to double/triple immunofluorescence/confocal microscopy of cultured cell lines, these techniques have recently evolved into high throughput platforms integrating new detection techniques, imaging software, and artificial intelligence analysis. Multiplexed imaging can now surpass the efficiency of cytofluorimetric analysis, as a similar information output (single-cell antigen coexpression quantification) is now linked to the spatial distribution of the analyzed markers in tissue/cell compartments. These techniques allow the precise identification of any single cell within a tissue and to dissect the complex communication network in which it is involved. The recent literature shows a boost in these techniques, which are frequently applied to cancer for microenvironmental or immunological studies.

This thematic issue would like to showcase the numerous multiplexed imaging techniques (from those affordable enough for any lab with standard equipment to more innovative examples) and to demonstrate their impact on cancer diagnosis and therapy. Studies on specific software and AI algorithms are also welcome, so long as they are applied to cancer.

Dr. Roberto Benelli
Guest Editor

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