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Bioengineering, Volume 10, Issue 6

June 2023 - 118 articles

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Cover Story: The market for human therapeutic and diagnostic agents is increasingly dominated by monoclonal antibodies (mAb) produced by Chinese hamster ovary (CHO) cells. Innovative monitoring technologies and process optimization have enabled a shift to continuous cultivation strategies and stable operations at high space–time yields. Due to challenges associated with product recovery and cell retention efficiency of membrane-based systems in high-cell density processes, however, there is also a rising demand for alternative devices. We present a 3D-printed microfluidic spiral separator as a “plug and play” cell-retention device capable of increasing mAb yield in a CHO cell perfusion process. Additionally, we detail the implementation of a web-based flow control mechanism to ensure process stability. View this paper

Articles (118)

  • Review
  • Open Access
7 Citations
3,930 Views
23 Pages

The Valley of Death confronts industrial biotechnology with a significant challenge to the commercialization of products. Fortunately, with the integration of computation, automation and artificial intelligence (AI) technology, the industrial biotech...

  • Article
  • Open Access
4 Citations
2,160 Views
16 Pages

Investigation of Stent Prototypes for the Eustachian Tube in Human Donor Bodies

  • Lena Rosenbusch,
  • Robert Schuon,
  • Tamara Wilfling,
  • Philipp Krüger,
  • Kerstin Lebahn,
  • Samuel John,
  • Olga Sahmel,
  • Niels Grabow,
  • Marko Schulze and
  • Andreas Wree
  • + 4 authors

Chronic otitis media is often connected to Eustachian tube dysfunction. As successful treatment cannot be guaranteed with the currently available options, the aim is to develop a stent for the Eustachian tube (ET). Over the course of this development...

  • Review
  • Open Access
4 Citations
3,140 Views
13 Pages

Deconstructing Fat to Reverse Radiation Induced Soft Tissue Fibrosis

  • Hannes Prescher,
  • Jill R. Froimson and
  • Summer E. Hanson

Adipose tissue is composed of a collection of cells with valuable structural and regenerative function. Taken as an autologous graft, these cells can be used to address soft tissue defects and irregularities, while also providing a reparative effect...

  • Review
  • Open Access
10 Citations
3,868 Views
17 Pages

Suppressing Chondrocyte Hypertrophy to Build Better Cartilage

  • Christian Shigley,
  • Jay Trivedi,
  • Ozair Meghani,
  • Brett D. Owens and
  • Chathuraka T. Jayasuriya

Current clinical strategies for restoring cartilage defects do not adequately consider taking the necessary steps to prevent the formation of hypertrophic tissue at injury sites. Chondrocyte hypertrophy inevitably causes both macroscopic and microsco...

  • Article
  • Open Access
3 Citations
2,114 Views
12 Pages

Quantification of Analgesic and Anti-Inflammatory Lipid Mediators in Long-Term Cryopreserved and Freeze-Dried Preserved Human Amniotic Membrane

  • Vladimir Vrkoslav,
  • Ingrida Smeringaiova,
  • Natalia Smorodinova,
  • Alzbeta Svobodova,
  • Stepan Strnad,
  • Catherine Joan Jackson,
  • Jan Burkert and
  • Katerina Jirsova

The aim of this study was to compare concentrations of endogenous N-acylethanolamine (NAE) lipid mediators—palmitoylethanolamide (PEA), oleoylethanolamide (OEA), and anandamide (AEA)—in fresh, decontaminated, cryopreserved, and freeze-dri...

  • Communication
  • Open Access
4 Citations
2,857 Views
11 Pages

Avermectins (AVMs), a family of 16-membered macrocyclic macrolides produced by Streptomyces avermitilis, have been the most successful microbial natural antiparasitic agents in recent decades. Doramectin, an AVM derivative produced by S. avermitilis...

  • Article
  • Open Access
12 Citations
2,489 Views
36 Pages

Colorectal cancer is associated with a high mortality rate and significant patient risk. Images obtained during a colonoscopy are used to make a diagnosis, highlighting the importance of timely diagnosis and treatment. Using techniques of deep learni...

  • Feature Paper
  • Article
  • Open Access
5 Citations
3,015 Views
22 Pages

Backgrounds and Objective: Facial palsy is a complex pathophysiological condition affecting the personal and professional lives of the involved patients. Sudden muscle weakness or paralysis needs to be rehabilitated to recover a symmetric and express...

  • Article
  • Open Access
6 Citations
3,281 Views
16 Pages

An Integrated Method of Biomechanics Modeling for Pelvic Bone and Surrounding Soft Tissues

  • Wei Kou,
  • Yefeng Liang,
  • Zhixing Wang,
  • Qingxi Liang,
  • Lining Sun and
  • Shaolong Kuang

The pelvis and its surrounding soft tissues create a complicated mechanical environment that greatly affects the success of fixing broken pelvic bones with surgical navigation systems and/or surgical robots. However, the modeling of the pelvic struct...

  • Article
  • Open Access
7 Citations
3,131 Views
21 Pages

Biocompatible MgFeCO3 Layered Double Hydroxide (LDH) for Bone Regeneration—Low-Temperature Processing through Cold Sintering and Freeze-Casting

  • Hyoung-Jun Kim,
  • Prescillia Lagarrigue,
  • Jae-Min Oh,
  • Jérémy Soulié,
  • Fabrice Salles,
  • Sophie Cazalbou and
  • Christophe Drouet

Layered Double Hydroxides (LDHs) are inorganic compounds of relevance to various domains, where their surface reactivity and/or intercalation capacities can be advantageously exploited for the retention/release of ionic and molecular species. In this...

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Bioengineering - ISSN 2306-5354Creative Common CC BY license