Table of Contents
Plants, Volume 9, Issue 4 (April 2020) – 144 articles
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Cover Story (view full-size image) Catharanthus roseus is the source of powerful anticancer monoterpene indole alkaloids (MIAs), in [...] Read more. Catharanthus roseus is the source of powerful anticancer monoterpene indole alkaloids (MIAs), in part deriving from the 2C-methyl-D-erythritol 4-phosphate (MEP) pathway. Regarded as a model for its complexity, the MIA biosynthetic pathway presents a unique pattern of compartmentation at both the cellular and subcellular levels. In the present study, four genes encoding missing enzymes of the MEP pathway were cloned, and systematic analysis of transcript distribution and protein subcellular localization of the whole MEP pathway was performed. RNA in situ hybridization revealed that all these genes were expressed in internal phloem-associated parenchyma of young leaves, while transient expression of fluorescent protein fusions showed that all these enzymes were targeted to stroma and stromules of plastids. View this paper.