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Article

Transcriptome Profiling of Ornithogalum dubium Leaves and Flowers to Identify Key Carotenoid Genes for CRISPR Gene Editing

1
Institute of Plant Science, The Volcani Center, Agricultural Research Organization, Bet Dagan 50250, Israel
2
Newe Yaar Research Center, Agricultural Research Organization, Ramat Yishay 30095, Israel
*
Author to whom correspondence should be addressed.
Plants 2020, 9(4), 540; https://doi.org/10.3390/plants9040540
Received: 24 March 2020 / Revised: 12 April 2020 / Accepted: 20 April 2020 / Published: 21 April 2020
Ornithogalum dubium is a popular ornamental monocot native to South Africa with flower colors ranging from pure white to deep orange. Gene editing based on the CRISPR/Cas9 system has recently been shown to hold potential for color improvement in ornamental flower crops. To apply this approach to Ornithogalum color manipulation, genomic or transcriptomic data must first be collected. Here, cDNA libraries of O. dubium leaves and flowers were constructed and sequenced using the Illumina HiSeq 2500. Over 155 million 100-bp paired-end reads were assembled into a transcriptome database of 360,689 contigs, of which 18,660 contigs were differentially expressed between leaves and flowers. Carotenoids are the main pigment imparting spectrum of orange hues to O. dubium flowers. By querying our database, we identified a total of 16 unique transcripts (unigenes) predicted to be involved in the carotenoid biosynthesis pathway of Ornithogalum. Combining carotenoid profiles, we further inferred several key unigenes responsible for floral coloration and accumulation in O. dubium, of which the gene LCYB/comp146645_c0 was found as a suitable target to generate potentially red flower varieties of O. dubium. Our research thus provides a framework for the application of CRISPR/Cas9 technology to improve this ornamental crop. View Full-Text
Keywords: carotenoid pathway; ornamental; Ornithogalum dubium; transcriptome carotenoid pathway; ornamental; Ornithogalum dubium; transcriptome
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MDPI and ACS Style

Wei, Z.; Arazi, T.; Hod, N.; Zohar, M.; Isaacson, T.; Doron-Faigenboim, A.; Reznik, N.; Yedidia, I. Transcriptome Profiling of Ornithogalum dubium Leaves and Flowers to Identify Key Carotenoid Genes for CRISPR Gene Editing. Plants 2020, 9, 540. https://doi.org/10.3390/plants9040540

AMA Style

Wei Z, Arazi T, Hod N, Zohar M, Isaacson T, Doron-Faigenboim A, Reznik N, Yedidia I. Transcriptome Profiling of Ornithogalum dubium Leaves and Flowers to Identify Key Carotenoid Genes for CRISPR Gene Editing. Plants. 2020; 9(4):540. https://doi.org/10.3390/plants9040540

Chicago/Turabian Style

Wei, Zunzheng, Tzahi Arazi, Nofar Hod, Matat Zohar, Tal Isaacson, Adi Doron-Faigenboim, Noam Reznik, and Iris Yedidia. 2020. "Transcriptome Profiling of Ornithogalum dubium Leaves and Flowers to Identify Key Carotenoid Genes for CRISPR Gene Editing" Plants 9, no. 4: 540. https://doi.org/10.3390/plants9040540

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