Table of Contents
Nanomaterials, Volume 9, Issue 3 (March 2019)
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Cover Story (view full-size image) We report the successful integration of the split-Spinach aptamer and DFHBI fluorophore pair into [...] Read more. We report the successful integration of the split-Spinach aptamer and DFHBI fluorophore pair into the six-stranded hexagonal shaped RNA nanoring. Destabilization of the helices directly adjacent to the aptamer core prohibits fluorescence in the absence of the fully-assembled nanoparticle. Nanoring assembly restores the aptamer’s binding pocket to trigger fluorescence of the bound fluorophore. The resulting system provides the ability to confirm the assembly of the six contiguous RNA strands—mediated entirely through their tertiary kissing-loop interactions. Finally, the integrated split-aptamer can monitor nanoparticle assembly of transcripts made directly from DNA templates isothermally in one-pot. View this paper.