Next Article in Journal
Solid Lipid Nanoparticles Surface Modification Modulates Cell Internalization and Improves Chemotoxic Treatment in an Oral Carcinoma Cell Line
Previous Article in Journal
A DFT Study of Hydrogen Storage in High-Entropy Alloy TiZrHfScMo
Open AccessArticle

Poly-ε-Caprolactone/Gelatin Hybrid Electrospun Composite Nanofibrous Mats Containing Ultrasound Assisted Herbal Extract: Antimicrobial and Cell Proliferation Study

1
Center for Environmental Nuclear Research, SRM Institute of Science and Technology, Kattankulathur Campus, Kancheepuram, Tamilnadu 603203, India
2
Department of Physics and Nanotechnology, SRM Institute of Science and Technology, Kattankulathur Campus, Kancheepuram, Tamilnadu 603203, India
3
Center for Nanofibers and Nanotechnology, Department of Mechanical Engineering, Faculty of Engineering, 2 Engineering Drive 3, National University of Singapore, Singapore 117576, Singapore
4
Anti-Infectives Research Group, Singapore Eye Research Institute, The Academia, 20 College Road, Discovery Tower, Singapore 169856, Singapore
5
Ophthalmology and Visual Sciences Academic Clinical Program, Duke-NUS Graduate Medical School, Singapore 169857, Singapore
6
Department of Biomedical Engineering, National University of Singapore, Singapore 117581, Singapore
7
Lee Kong Chian School of Medicine, Nanyang Technological University Singapore, Experimental Medicine Building, 59 Nanyang Drive, Singapore 636921, Singapore
8
Department of Anatomy, SRM Medical College Hospital and Research Centre, Kattankulathur Campus, Kancheepuram, Tamilnadu 603203, India
9
Materials Physics Division, Material Science Group, Indira Gandhi Centre for Atomic Research, Kalpakkam, Tamilnadu 603102, India
10
Skin Research Institute of Singapore, 8A Biomedical Grove, #06-06 Immunos, Singapore 138648, Singapore
*
Authors to whom correspondence should be addressed.
Nanomaterials 2019, 9(3), 462; https://doi.org/10.3390/nano9030462
Received: 7 February 2019 / Revised: 25 February 2019 / Accepted: 1 March 2019 / Published: 20 March 2019
Electrospun fibers have emerged as promising materials in the field of biomedicine, due to their superior physical and cell supportive properties. In particular, electrospun mats are being developed for advanced wound dressing applications. Such applications require the firers to possess excellent antimicrobial properties in order to inhibit potential microbial colonization from resident and non-resident bacteria. In this study, we have developed Poly-ε-Caprolactone /gelatin hybrid composite mats loaded with natural herbal extract (Gymnema sylvestre) to prevent bacterial colonization. As-spun scaffolds exhibited good wettability and desirable mechanical properties retaining their fibrous structure after immersing them in phosphate buffered saline (pH 7.2) for up to 30 days. The initial burst release of Gymnema sylvestre prevented the colonization of bacteria as confirmed by the radial disc diffusion assay. Furthermore, the electrospun mats promoted cellular attachment, spreading and proliferation of human primary dermal fibroblasts and cultured keratinocytes, which are crucial parenchymal cell-types involved in the skin recovery process. Overall these results demonstrated the utility of Gymnema sylvestre impregnated electrospun PCL/Gelatin nanofibrous mats as an effective antimicrobial wound dressing. View Full-Text
Keywords: electrospun hybrid mats; ultrasound assisted extraction; anti-infective wound dressing; poly-ε-caprolactone; gelatin electrospun hybrid mats; ultrasound assisted extraction; anti-infective wound dressing; poly-ε-caprolactone; gelatin
Show Figures

Graphical abstract

MDPI and ACS Style

Ramalingam, R.; Dhand, C.; Leung, C.M.; Ezhilarasu, H.; Prasannan, P.; Ong, S.T.; Subramanian, S.; Kamruddin, M.; Lakshminarayanan, R.; Ramakrishna, S.; Verma, N.K.; Arunachalam, K.D. Poly-ε-Caprolactone/Gelatin Hybrid Electrospun Composite Nanofibrous Mats Containing Ultrasound Assisted Herbal Extract: Antimicrobial and Cell Proliferation Study. Nanomaterials 2019, 9, 462.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop