Table of Contents
Molecules, Volume 25, Issue 11 (June-1 2020) – 256 articles
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Cover Story (view full-size image) Honey was extracted with solvent and the lipid fractions purified by HPLC prior to analysis by qTOF [...] Read more. Honey was extracted with solvent and the lipid fractions purified by HPLC prior to analysis by qTOF LC–MS, resulting in the detection of 20-hydroxy-7-deydrocholesterol, vitamin D3, lumisterol, and species corresponding to 20-hydroxy-7-dehydrocholesterol, 20-hydroxyvitamin D3, 1,20-dihydroxyvitamin D3, 25-hydroxyvitamin D3, and 1,25-dihydroxyvitamin D3. The quantification of one of the major products, 20-hydroxyvitamin D3, revealed a concentration in dietary honey that could potentially display biological activity in humans. The detection of vitamin D metabolites in honey implies that 7-dehydrocholesterol undergoes phototransformation to vitamin D in bees, consistent with previous studies, with prior or subsequent enzymatic hydroxylation at C20, C25, and/or C1α. View this paper.