Special Issue "Innovation in Propagation of Fruit, Vegetable and Ornamental Plants"

A special issue of Horticulturae (ISSN 2311-7524).

Deadline for manuscript submissions: closed (31 May 2019)

Special Issue Editor

Guest Editor
Prof. Dr. Sergio Ruffo Roberto

Agricultural Research Center, Londrina State University, Celso Garcia Cid Road, km 380, P.O. Box 10.011, Londrina ZIP 86057-970, PR, Brazil
Website | E-Mail
Interests: agronomy; fruit crops; plant propagation; post-harvest of fruits; plant growth regulators

Special Issue Information

Dear Colleagues,

In horticulture, plant propagation plays an important role as the number of plants can be rapidly multiplied retaining desirable characteristics of the mother plants, and shortening the bearing age of plants. There are two primary forms of plant propagation: sexual and asexual. In nature, propagation of plants most often involves sexual reproduction, and this form is still used in several species. Over the years, horticulturists have developed asexual propagation methods that use vegetative plant parts. Innovation in plant propagation has supported breeding programs and allowed the production of high quality nursery plants with the same genetic characteristics of the mother plant, free of diseases or pests.

The purpose of this Special Issue “Innovation in Propagation of Fruit, Vegetable and Ornamental Plants” aims to present state-of-the-art techniques recently developed by researchers worldwide. Innovative articles on propagation of any fruit, vegetable and ornamental species are welcome in this Special Issue.

Prof. Sergio Ruffo Roberto
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Horticulturae is an international peer-reviewed open access quarterly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) is waived for well-prepared manuscripts submitted to this issue. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • propagation methods
  • plant growth media
  • cuttings
  • seeds
  • tissue culture
  • nursery plants
  • growth regulators

Published Papers (5 papers)

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Research

Open AccessFeature PaperArticle
LEDs Combined with CHO Sources and CCC Priming PLB Regeneration of Phalaenopsis
Horticulturae 2019, 5(2), 34; https://doi.org/10.3390/horticulturae5020034
Received: 19 February 2019 / Revised: 15 April 2019 / Accepted: 17 April 2019 / Published: 6 May 2019
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Abstract
Throughout this study, the objective was to determine the most effective carbohydrate (CHO) sources under different light-emitting diodes (LEDs), and the impact of chlorocholine chloride (CCC), for the in vitro regeneration of the protocom-like bodies (PLBs) in Phalaenopsis ‘Fmk02010’. We applied 15 LEDs [...] Read more.
Throughout this study, the objective was to determine the most effective carbohydrate (CHO) sources under different light-emitting diodes (LEDs), and the impact of chlorocholine chloride (CCC), for the in vitro regeneration of the protocom-like bodies (PLBs) in Phalaenopsis ‘Fmk02010’. We applied 15 LEDs combined with three CHO sources and five CCC concentrations in the study. Organogenesis of PLBs was very poor in maltose both for the number of PLBs and their fresh weight (FW) compared to media containing sucrose and trehalose. Sucrose was the best CHO source under the red-white (RW) LED for the in vitro organogenesis of PLBs (PLBs: 54.13; FW: 0.109 g), while trehalose was best under the blue-white (BW) LED (PLBs: 36.33, FW: 0.129 g). The red-blue-white (RBW)-trehalose combination generated a suitable number of PLBs (35.13) with the highest FW (0.167 g). CCC at 0.01, 0.1, and 1 mgL−1CCC had no effect on PLB formation or FW, but 10 mg L−1 reduced both. RW-sucrose, BW-trehalose, and RBW-trehalose were the best combinations for PLB organogenesis. The addition of low concentrations of CCC in the plant culture medium are unnecessary. Full article
(This article belongs to the Special Issue Innovation in Propagation of Fruit, Vegetable and Ornamental Plants)
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Open AccessFeature PaperCommunication
Aeroponic Cloning of Capsicum spp.
Horticulturae 2019, 5(2), 30; https://doi.org/10.3390/horticulturae5020030
Received: 9 March 2019 / Accepted: 10 April 2019 / Published: 16 April 2019
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Abstract
Aeroponic cloning is a great strategy to maintain desired genotypes by generating a whole new plant from cuttings. While this propagation technique has been demonstrated for tomatoes (Solanum lycopersicum) and potatoes (Solanum tuberosum), no protocol has been developed for [...] Read more.
Aeroponic cloning is a great strategy to maintain desired genotypes by generating a whole new plant from cuttings. While this propagation technique has been demonstrated for tomatoes (Solanum lycopersicum) and potatoes (Solanum tuberosum), no protocol has been developed for peppers (Capsicum spp.). The ability to clonally propagate different Capsicum holds promise for domestic and industrial growing operations since elite cultivars with desirable traits (e.g., high capsaicin levels, nutrient content, and striped fruit) can be perpetuated without the need of planning a nursery. We tested six Capsicum species for their feasibility of aeroponic cloning by stem cuttings. All domestic species were successfully regenerated under aeroponic conditions but not for Capsicum eximium, a wild species. Of the species analyzed, Capsicum annuum peppers had the fastest node formation (11.6 +/− 0.89 days, P ≤ 0.01) and obtained a larger volume of roots (P ≤ 0.01) after node formation as compared to C. baccatum, C. frutescens, and C. pubescens. This study presents a cost-effective strategy to clonally propagate peppers for personal, industrial, and conservation purposes. Full article
(This article belongs to the Special Issue Innovation in Propagation of Fruit, Vegetable and Ornamental Plants)
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Open AccessArticle
Propagation from Basal Epicormic Meristems Remediates an Aging-Related Disorder in Almond Clones
Horticulturae 2019, 5(2), 28; https://doi.org/10.3390/horticulturae5020028
Received: 5 February 2019 / Revised: 4 April 2019 / Accepted: 7 April 2019 / Published: 11 April 2019
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Abstract
The asexual propagation of clonal crops has allowed cultivation of superior selections for thousands of years. With time, some clones deteriorate from genetic and epigenetic changes. Non-infectious bud-failure (NBF) in cultivated almond (Prunus dulcis) is a commercially important age-related disorder that [...] Read more.
The asexual propagation of clonal crops has allowed cultivation of superior selections for thousands of years. With time, some clones deteriorate from genetic and epigenetic changes. Non-infectious bud-failure (NBF) in cultivated almond (Prunus dulcis) is a commercially important age-related disorder that results in the failure of new vegetative buds to grow in the spring, with dieback of terminal shoots, witches-brooming of surviving buds, and deformed bark (roughbark). The incidence of NBF increases with clone age, including within individual long-lived trees as well as nursery propagation lineages. It is not associated with any infectious disease agents. Consequently, nursery practices emphasize the establishment of foundation-mother blocks utilizing propagation-wood selected from proven and well-monitored propagation-lineages. Commercial propagation utilizes axillary shoot buds through traditional budding or grafting. This study examines NBF development using basal epicormic buds from individual trees of advanced age as an alternative source of foundation stock. Results show the age-related progression of NBF is suppressed in these epicormic meristems, possibly owing to their unique origins and ontogeny. NBF development in commercial orchards propagated from foundation blocks established from these sources was similarly dramatically suppressed even over the 10- to 20-year expected commercial orchard-life. Foundation-stock stability can be further maintained through appropriate management of propagation source-trees, which requires accurate knowledge of meristem origin and development. Full article
(This article belongs to the Special Issue Innovation in Propagation of Fruit, Vegetable and Ornamental Plants)
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Open AccessArticle
In Vitro Establishment of ‘Delite’ Rabbiteye Blueberry Microshoots
Horticulturae 2019, 5(1), 24; https://doi.org/10.3390/horticulturae5010024
Received: 18 January 2019 / Revised: 20 February 2019 / Accepted: 25 February 2019 / Published: 8 March 2019
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Abstract
Micropropagation is an important technique for clonal mass propagation and a tool for in vitro studies. One of the first steps to overcome in this process is the establishment of new explants in vitro. ‘Delite’ rabbiteye blueberry was cultured in vitro with four [...] Read more.
Micropropagation is an important technique for clonal mass propagation and a tool for in vitro studies. One of the first steps to overcome in this process is the establishment of new explants in vitro. ‘Delite’ rabbiteye blueberry was cultured in vitro with four cytokinins (zeatin (ZEA), 6-(γ-γ-dimethylallylamino)-purine (2iP), 6-benzylaminopurine (BAP), and kinetin (KIN)) at eight concentrations (0, 2.5, 5, 10, 20, 30, 40, and 50 µM). Additionally, nine combinations of nitrogen salts were tested, using Woody Plant Medium (WPM) and a modified WPM as the basic medium. ZEA and 2iP showed better responses, but ZEA was superior at lower (2.5 µM) concentrations (89.7% survival, 81.3% shoot formation, 1.3 shoots, 13.8 mm shoot length, 10.0 leaves). BAP and KIN showed very low responses. In the combinations of salts with modified WPM, no differences were observed. However, the original WPM with treatments of 0.5 × NH4NO3 and 1 × Ca(NO3)2, 0.5 × NH4NO3 and 0.5 × Ca(NO3)2, and the modified WPM alone showed the lowest rates of survival and shoot formation and the shortest shoot lengths. The highest shoot lengths were observed in treatments with the original WPM, 1.5 × NH4NO3 and 0.5 × Ca(NO3)2, and 1.5 × NH4NO3 and 1.5 × Ca(NO3)2. This initial study with ‘Delite’ can be the basis for further experiments with different combinations of salts, 2iP, and ZEA. Full article
(This article belongs to the Special Issue Innovation in Propagation of Fruit, Vegetable and Ornamental Plants)
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Open AccessFeature PaperCommunication
Pecan Propagation: Seed Mass as a Reliable Tool for Seed Selection
Horticulturae 2018, 4(3), 26; https://doi.org/10.3390/horticulturae4030026
Received: 26 August 2018 / Revised: 2 September 2018 / Accepted: 6 September 2018 / Published: 11 September 2018
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Abstract
Pecan is one of the most important horticultural nut crops in the world. It is a deciduous species native to the temperate zones of North America, introduced into the subtropical regions of Brazil during the 1870s. High quality seedlings are essential to establishing [...] Read more.
Pecan is one of the most important horticultural nut crops in the world. It is a deciduous species native to the temperate zones of North America, introduced into the subtropical regions of Brazil during the 1870s. High quality seedlings are essential to establishing healthy and productive orchards, and selection of seeds is an important factor in this issue. In this study we evaluated the correlation between seed mass, emergence rate and morphometric traits of seedlings in the pecan cultivar Importada. A significant positive correlation (r > 0.81) between seed mass and plantlet height, stem diameter, emergence rate and number of leaves was observed. Our results suggest that seed mass can be used as a direct method for seed selection towards production of vigorous pecan seedlings. However, since an increase in seed mass is usually associated with a decrease in the number of seeds that a plant can produce per unit canopy, long-duration studies are recommended in order to evaluate the influence of seed selection on a plantation’s production. Full article
(This article belongs to the Special Issue Innovation in Propagation of Fruit, Vegetable and Ornamental Plants)
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