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LEDs Combined with CHO Sources and CCC Priming PLB Regeneration of Phalaenopsis

1
The United Graduate School of Agricultural Sciences, Ehime University, Ehime 790-8577, Japan
2
Faculty of Agriculture, Kochi University, Kochi 783-8502, Japan
*
Author to whom correspondence should be addressed.
Horticulturae 2019, 5(2), 34; https://doi.org/10.3390/horticulturae5020034
Received: 19 February 2019 / Revised: 15 April 2019 / Accepted: 17 April 2019 / Published: 6 May 2019
(This article belongs to the Special Issue Innovation in Propagation of Fruit, Vegetable and Ornamental Plants)
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Abstract

Throughout this study, the objective was to determine the most effective carbohydrate (CHO) sources under different light-emitting diodes (LEDs), and the impact of chlorocholine chloride (CCC), for the in vitro regeneration of the protocom-like bodies (PLBs) in Phalaenopsis ‘Fmk02010’. We applied 15 LEDs combined with three CHO sources and five CCC concentrations in the study. Organogenesis of PLBs was very poor in maltose both for the number of PLBs and their fresh weight (FW) compared to media containing sucrose and trehalose. Sucrose was the best CHO source under the red-white (RW) LED for the in vitro organogenesis of PLBs (PLBs: 54.13; FW: 0.109 g), while trehalose was best under the blue-white (BW) LED (PLBs: 36.33, FW: 0.129 g). The red-blue-white (RBW)-trehalose combination generated a suitable number of PLBs (35.13) with the highest FW (0.167 g). CCC at 0.01, 0.1, and 1 mgL−1CCC had no effect on PLB formation or FW, but 10 mg L−1 reduced both. RW-sucrose, BW-trehalose, and RBW-trehalose were the best combinations for PLB organogenesis. The addition of low concentrations of CCC in the plant culture medium are unnecessary. View Full-Text
Keywords: protocorm-like bodies; light-emitting diode; trehalose; maltose; CCC; correlation; growth retardants protocorm-like bodies; light-emitting diode; trehalose; maltose; CCC; correlation; growth retardants
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Mehraj, H.; Alam, M.M.; Habiba, S.U.; Mehbub, H. LEDs Combined with CHO Sources and CCC Priming PLB Regeneration of Phalaenopsis. Horticulturae 2019, 5, 34.

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