Next Article in Journal
Risk of Human Pathogen Internalization in Leafy Vegetables During Lab-Scale Hydroponic Cultivation
Next Article in Special Issue
Propagation from Basal Epicormic Meristems Remediates an Aging-Related Disorder in Almond Clones
Previous Article in Journal
Postharvest Dips of Persimmon Fruit in Gibberellic Acid: An Efficient Treatment to Improve Storability and Reduce Alternaria Black Spot
Previous Article in Special Issue
Pecan Propagation: Seed Mass as a Reliable Tool for Seed Selection
Article Menu
Issue 1 (March) cover image

Export Article

Open AccessArticle

In Vitro Establishment of ‘Delite’ Rabbiteye Blueberry Microshoots

Departamento de Fitotecnia e Fitossanitarismo, Universidade Federal do Paraná, Rua dos Funcionários, 1540. CEP 80.035-050, Curitiba, Brazil
Author to whom correspondence should be addressed.
Horticulturae 2019, 5(1), 24;
Received: 18 January 2019 / Revised: 20 February 2019 / Accepted: 25 February 2019 / Published: 8 March 2019
(This article belongs to the Special Issue Innovation in Propagation of Fruit, Vegetable and Ornamental Plants)
PDF [2855 KB, uploaded 13 March 2019]


Micropropagation is an important technique for clonal mass propagation and a tool for in vitro studies. One of the first steps to overcome in this process is the establishment of new explants in vitro. ‘Delite’ rabbiteye blueberry was cultured in vitro with four cytokinins (zeatin (ZEA), 6-(γ-γ-dimethylallylamino)-purine (2iP), 6-benzylaminopurine (BAP), and kinetin (KIN)) at eight concentrations (0, 2.5, 5, 10, 20, 30, 40, and 50 µM). Additionally, nine combinations of nitrogen salts were tested, using Woody Plant Medium (WPM) and a modified WPM as the basic medium. ZEA and 2iP showed better responses, but ZEA was superior at lower (2.5 µM) concentrations (89.7% survival, 81.3% shoot formation, 1.3 shoots, 13.8 mm shoot length, 10.0 leaves). BAP and KIN showed very low responses. In the combinations of salts with modified WPM, no differences were observed. However, the original WPM with treatments of 0.5 × NH4NO3 and 1 × Ca(NO3)2, 0.5 × NH4NO3 and 0.5 × Ca(NO3)2, and the modified WPM alone showed the lowest rates of survival and shoot formation and the shortest shoot lengths. The highest shoot lengths were observed in treatments with the original WPM, 1.5 × NH4NO3 and 0.5 × Ca(NO3)2, and 1.5 × NH4NO3 and 1.5 × Ca(NO3)2. This initial study with ‘Delite’ can be the basis for further experiments with different combinations of salts, 2iP, and ZEA. View Full-Text
Keywords: Ericaceae; Vaccinium virgatum; micropropagation; in vitro culture; cytokinins; zeatin; 2iP; BAP; kinetin; WPM Ericaceae; Vaccinium virgatum; micropropagation; in vitro culture; cytokinins; zeatin; 2iP; BAP; kinetin; WPM

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Supplementary material


Share & Cite This Article

MDPI and ACS Style

Schuchovski, C.S.; Biasi, L.A. In Vitro Establishment of ‘Delite’ Rabbiteye Blueberry Microshoots. Horticulturae 2019, 5, 24.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Horticulturae EISSN 2311-7524 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top