Novel Approaches for Improving the Microbial Quality of Foods

A special issue of Foods (ISSN 2304-8158). This special issue belongs to the section "Dairy".

Deadline for manuscript submissions: closed (30 November 2020) | Viewed by 4237

Special Issue Editor


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Guest Editor
Department of Food Sciences, Agricultural Research Organization—The Volcani Center, Derech Hamacabim, POB 15159, Rishon LeZion 7528809, Israel
Interests: biofilm; spore-forming Bacillus; dairy food; probiotic bacilli

Special Issue Information

Dear Colleagues,

Microbial damage caused by biofilm bacteria in the dairy industry is a fundamental threat to the safety and quality of milk products. Many bacteria in industrial settings tend to form multicellular communities known as biofilms. Bacterial cells are much protected in the biofilms due to a self-produced matrix that consists mainly of sugars and proteins, which form a physical barrier. Biofilms are not only a potential source of contamination, but can also increase corrosion rate, reduce heat transfer, and increase fluid frictional resistance. Therefore, mitigation of biofilm-forming species will enable the development of novel means and technologies for preventing biofilm formation and subsequent contamination of dairy products.

Dr. Moshe Shemesh
Guest Editor

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Keywords

  • biofilm
  • endospores
  • antibiofilm surfaces
  • sanitizing

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Published Papers (1 paper)

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Research

15 pages, 2532 KiB  
Article
Mitigating Milk-Associated Bacteria through Inducing Zinc Ions Antibiofilm Activity
by Carmel Hutchings, Satish Kumar Rajasekharan, Ram Reifen and Moshe Shemesh
Foods 2020, 9(8), 1094; https://doi.org/10.3390/foods9081094 - 11 Aug 2020
Cited by 17 | Viewed by 3885
Abstract
Dairy products are a sector heavily impacted by food loss, often due to bacterial contaminations. A major source of contamination is associated with the formation of biofilms by bacterial species adopted to proliferate in milk production environment and onto the surfaces of milk [...] Read more.
Dairy products are a sector heavily impacted by food loss, often due to bacterial contaminations. A major source of contamination is associated with the formation of biofilms by bacterial species adopted to proliferate in milk production environment and onto the surfaces of milk processing equipment. Bacterial cells within the biofilm are characterized by increased resistance to unfavorable environmental conditions and antimicrobial agents. Members of the Bacillus genus are the most commonly found spoilage microorganisms in the dairy environment. It appears that physiological behavior of these species is somehow depended on the availability of bivalent cations in the environment. One of the important cations that may affect the bacterial physiology as well as survivability are Zn2+ ions. Thus, the aim of this study was to examine the antimicrobial effect of Zn2+ ions, intending to elucidate the potential of a zinc-based antibacterial treatment suitable for the dairy industry. The antimicrobial effect of different doses of ZnCl2 was assessed microscopically. In addition, expression of biofilm related genes was evaluated using RT-PCR. Analysis of survival rates following heat treatment was conducted in order to exemplify a possible applicative use of Zn2+ ions. Addition of zinc efficiently inhibited biofilm formation by B. subtilis and further disrupted the biofilm bundles. Expression of matrix related genes was found to be notably downregulated. Microscopic evaluation showed that cell elongation was withheld when cells were grown in the presence of zinc. Finally, B. cereus and B. subtilis cells were more susceptible to heat treatment after being exposed to Zn2+ ions. It is believed that an anti-biofilm activity, expressed in downregulation of genes involved in construction of the extracellular matrix, would account for the higher sensitivity of bacteria during heat pasteurization. Consequently, we suggest that Zn2+ ions can be of used as an effective antimicrobial treatment in various applications in the dairy industry, targeting both biofilms and vegetative bacterial cells. Full article
(This article belongs to the Special Issue Novel Approaches for Improving the Microbial Quality of Foods)
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