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Bioengineering, Volume 9, Issue 2

February 2022 - 43 articles

Cover Story: The established structure-property relationship and biochemical composition of major ampullate spider silk provides it with an impressive balance of strength and elasticity, unrivaled by synthetic materials. Other biologically inspired small peptide motifs correlated to specific functionalities can be combined with this unit to create designer silk material with new hybrid properties. Four concatenated repeats of the heparin-binding motif, ARKKAAKA, were genetically linked with MaSp2 amino acid motifs (GPGXXAn) to produce a mechanically robust protein capable of both binding biologically active heparin and resisting pathogen colonization, potentially enabling both in vivo anticoagulation and anti-infectivity. The anticoagulant activity of this fusion protein was comparable to the activity of surface-bound heparin. View this paper
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Articles (43)

  • Review
  • Open Access
23 Citations
8,955 Views
21 Pages

Measuring Biosignals with Single Circuit Boards

  • Guido Ehrmann,
  • Tomasz Blachowicz,
  • Sarah Vanessa Homburg and
  • Andrea Ehrmann

To measure biosignals constantly, using textile-integrated or even textile-based electrodes and miniaturized electronics, is ideal to provide maximum comfort for patients or athletes during monitoring. While in former times, this was usually solved b...

  • Article
  • Open Access
23 Citations
7,681 Views
12 Pages

The emergence of CRISPR-Cas9 technology has revolutionized both basic and translational biomedical research. For Cas9 nuclease to exert genome editing activity, nuclear localization signal (NLS) derived from simian virus 40 (SV40) T antigen is common...

  • Review
  • Open Access
62 Citations
5,836 Views
17 Pages

Development of Novel Tetrapyrrole Structure Photosensitizers for Cancer Photodynamic Therapy

  • Natalia Plekhova,
  • Olga Shevchenko,
  • Oksana Korshunova,
  • Aleksandra Stepanyugina,
  • Ivan Tananaev and
  • Vladimir Apanasevich

The effectiveness of photodynamic therapy (PDT) is based on the triad effects of photosensitizer (PS), molecular oxygen and visible light on malignant tumors. Such complex induces a multifactorial manner including reactive-oxygen-species-mediated dam...

  • Article
  • Open Access
3 Citations
5,612 Views
16 Pages

Cell segmentation is a critical step for image-based experimental analysis. Existing cell segmentation methods are neither entirely automated nor perform well under basic laboratory microscopy. This study proposes an efficient and automated cell segm...

  • Article
  • Open Access
7 Citations
3,103 Views
15 Pages

A Multiscale Partition-Based Kolmogorov–Sinai Entropy for the Complexity Assessment of Heartbeat Dynamics

  • Andrea Scarciglia,
  • Vincenzo Catrambone,
  • Claudio Bonanno and
  • Gaetano Valenza

Background: Several methods have been proposed to estimate complexity in physiological time series observed at different time scales, with a particular focus on heart rate variability (HRV) series. In this frame, while several complexity quantifiers...

  • Article
  • Open Access
2 Citations
3,460 Views
15 Pages

Environmental Factors Modulate the Role of orf21 Sigma Factor in Clavulanic Acid Production in Streptomyces Clavuligerus ATCC27064

  • Luisa F. Patiño,
  • Vanessa Aguirre-Hoyos,
  • Laura I. Pinilla,
  • León F. Toro and
  • Rigoberto Ríos-Estepa

Sigma factors and sigma factor-related mechanisms control antibiotic production in Streptomyces. In this contribution, the orf21 gene was overexpressed in the wild-type strain of Streptomyces clavuligerus ATCC2764, yielding S. clavuligerus/pIORF21, t...

  • Article
  • Open Access
24 Citations
5,974 Views
18 Pages

Bioleaching of Heavy Metals from Printed Circuit Boards with an Acidophilic Iron-Oxidizing Microbial Consortium in Stirred Tank Reactors

  • Juan Tapia,
  • Alex Dueñas,
  • Nick Cheje,
  • Gonzalo Soclle,
  • Nila Patiño,
  • Wendy Ancalla,
  • Sara Tenorio,
  • Jorge Denos,
  • Homar Taco and
  • Weiwei Cao
  • + 5 authors

In this study, bioleaching was carried out for the recovery of metals (copper, zinc, tin, lead, gold and silver) from printed circuit boards residues (PCBs), one of the most important wastes from electrical and electronic equipment, using an acidophi...

  • Article
  • Open Access
7 Citations
4,049 Views
15 Pages

A Second-Generation Nanoluc-IL27 Fusion Cytokine for Targeted-Gene-Therapy Applications

  • Janelle Wesleyn Salameh,
  • Shreya Kumar,
  • Cosette Marie Rivera-Cruz and
  • Marxa Leao Figueiredo

An emerging approach in treating skeletal malignancies utilizes osteoimmunology to investigate new multifunctional immune-stimulatory agents that can simultaneously combat tumor growth and promote bone repair. We have hypothesized that cytokine Inter...

  • Article
  • Open Access
1 Citations
2,734 Views
12 Pages

Biomechanical Evaluation of Recurrent Dissociation of Modular Humeral Prostheses

  • Daniel B. Luckenbill,
  • Mike F. Iossi,
  • Alyssa M. George Whitney,
  • Danielle Miller,
  • Lynn A. Crosby and
  • Tarun Goswami

The purpose of the study was to evaluate the force and torque required to dissociate a humeral head from the unimplanted modular total shoulder replacement system from different manufacturers and to determine if load and torque to dissociation are re...

  • Review
  • Open Access
22 Citations
10,717 Views
14 Pages

The History and Innovations of Blood Vessel Anastomosis

  • William R. Moritz,
  • Shreya Raman,
  • Sydney Pessin,
  • Cameron Martin,
  • Xiaowei Li,
  • Amanda Westman and
  • Justin M. Sacks

Surgical technique and technology frequently coevolve. The brief history of blood vessel anastomosis is full of famous names. While the techniques pioneered by these surgeons have been well described, the technology that facilitated their advancement...

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Bioengineering - ISSN 2306-5354