Liquid fermentation is an efficient culture for obtaining polysaccharides from edible mushrooms. In this study, the polysaccharide content and biomass were examined by introducing microorganisms into the 
Wolfiporia cocos fermentation system. Three edible mushroom co-fermentation systems were established, among which the 
Wolfiporia cocos-Ganoderma
             [...] Read more.
         
        
            Liquid fermentation is an efficient culture for obtaining polysaccharides from edible mushrooms. In this study, the polysaccharide content and biomass were examined by introducing microorganisms into the 
Wolfiporia cocos fermentation system. Three edible mushroom co-fermentation systems were established, among which the 
Wolfiporia cocos-Ganoderma lucidum co-fermentation system significantly increased the mycelial biomass of the system by 57.71% compared to 
Wolfiporia cocos alone and 91.22% compared to 
Ganoderma lucidum alone, and the intracellular polysaccharide content was significantly increased. Physiological activities of polysaccharides showed that mycelial polysaccharides in the 
Wolfiporia cocos-Ganoderma lucidum system had stronger anti-tumor cell value-adding and anti-tumor cell migration activities compared with 
Wolfiporia cocos and 
Ganoderma lucidum fermentation alone. The transcriptomic study of 
Wolfiporia cocos mycelium induced by exogenous substances suggested that the exogenous substances could enhance the intracellular polysaccharide content of 
Wolfiporia cocos through the upregulation of the expression of α-glycosyltransferase encoded by ALG10 and the downregulation of α-glycosidases encoded by MAN1B in the glycolytic metabolism of 
Wolfiporia cocos. This study provides a new direction for the transformation of polysaccharides from 
Wolfiporia cocos and 
Ganoderma lucidum into functional foods and new product development, and provides an experimental basis.
            
Full article