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Biosensors, Volume 7, Issue 4

December 2017 - 23 articles

Cover Story: A biosensor for 3-hydroxybutyrate (3-HB) involving immobilization of the enzyme 3-hydroxybutyrate dehydrogenase onto a screen-printed carbon electrode modified with reduced graphene oxide (GO) and thionine (THI) is reported here. After addition of 3-hydroxybutyrate, or the sample, in the presence of NAD+ cofactor, the generated NADH could be detected amperometrically at 0.0 V vs. Ag pseudo reference electrode. Under the optimized experimental conditions, a calibration plot for 3-HB was constructed showing a wide linear range between 0.010 and 0.400 mM 3-HB, which covers the clinically relevant levels for diluted serum samples. In addition, a limit of detection of 1.0 µM, much lower than that reported using other biosensors, was achieved. The analytical usefulness of the developed biosensor was demonstrated via application to spiked serum samples. View this paper

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Article

43 Citations

8,999 Views

12 Pages

Raman Computational and Experimental Studies of Dopamine Detection

John D. Ciubuc,
Kevin E. Bennet,
Chao Qiu,
Matthew Alonzo,
William G. Durrer and
Felicia S. Manciu

Biosensors2017, 7(4), 43;https://doi.org/10.3390/bios7040043

28 September 2017

A combined theoretical and experimental analysis of dopamine (DA) is presented in this work with the objective of achieving more accurate detection and monitoring of this neurotransmitter at very low concentrations, specific to physiological levels....

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Review

31 Citations

11,093 Views

18 Pages

A Review on Passive and Integrated Near-Field Microwave Biosensors

Subhajit Guha,
Farabi Ibne Jamal and
Christian Wenger

Biosensors2017, 7(4), 42;https://doi.org/10.3390/bios7040042

23 September 2017

In this paper we review the advancement of passive and integrated microwave biosensors. The interaction of microwave with biological material is discussed in this paper. Passive microwave biosensors are microwave structures, which are fabricated on a...

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Article

7 Citations

7,700 Views

11 Pages

Biosensor Technology Reveals the Disruption of the Endothelial Barrier Function and the Subsequent Death of Blood Brain Barrier Endothelial Cells to Sodium Azide and Its Gaseous Products

Dan T. Kho,
Rebecca H. Johnson,
Simon J. O’Carroll,
Catherine E. Angel and
E. Scott Graham

Biosensors2017, 7(4), 41;https://doi.org/10.3390/bios7040041

21 September 2017

Herein we demonstrate the sensitive nature of human blood-brain barrier (BBB) endothelial cells to sodium azide and its gaseous product. Sodium azide is known to be acutely cytotoxic at low millimolar concentrations, hence its use as a biological pre...

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