Development and Bioanalytical Applications of a White Light Reflectance Spectroscopy Label-Free Sensing Platform
by
Georgios Koukouvinos 1, Panagiota Petrou 1, Dimitrios Goustouridis 2,3, Konstantinos Misiakos 4, Sotirios Kakabakos 1 and Ioannis Raptis 2,4,*
Georgios Koukouvinos 1, Panagiota Petrou 1, Dimitrios Goustouridis 2,3, Konstantinos Misiakos 4, Sotirios Kakabakos 1 and Ioannis Raptis 2,4,*
1
Immunoassay/Immunosensors Lab, Institute of Nuclear & Radiological Sciences & Technology, Energy & Safety, NCSR “Demokritos”, 15310 Aghia Paraskevi, Greece
2
ThetaMetrisis S.A., 12243 Egaleo, Greece
3
Department of Electronics Engineering TEI of Piraeus, 12244 Egaleo, Greece
4
Optical sensors Lab, Institute of Nanoscience and Nanotechnology, NCSR “Demokritos”, 15310 Aghia Paraskevi, Greece
*
Author to whom correspondence should be addressed.
Biosensors 2017, 7(4), 46; https://doi.org/10.3390/bios7040046
Received: 13 July 2017 / Revised: 9 October 2017 / Accepted: 11 October 2017 / Published: 13 October 2017
(This article belongs to the Special Issue Point-of-Care Diagnostics)
The development of a sensing platform based on white light reflectance spectroscopy (WLRS) is presented. The evolution of the system, from polymer film characterization and sensing of volatile organic compounds to biosensor for the label-free determination of either high (e.g., proteins) or low molecular weight analytes (e.g., pesticides), is described. At the same time, the passage from single to multi-analyte determinations, and from a laboratory prototype set-up to a compact device appropriate for on-site determination, is outlined. The improvements made on both the sensor and the optical set-up, and the concomitant advances in the analytical characteristics and the robustness of the assays performed with the different layouts, are also presented. Finally, the future perspectives of the system, aiming for the creation of a standalone instrument to be used by non-experts, will be discussed.
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Keywords:
white light reflectance spectroscopy; label-free determinations; fast assays; volatile organic compounds; protein analytes; low molecular weight molecules
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MDPI and ACS Style
Koukouvinos, G.; Petrou, P.; Goustouridis, D.; Misiakos, K.; Kakabakos, S.; Raptis, I. Development and Bioanalytical Applications of a White Light Reflectance Spectroscopy Label-Free Sensing Platform. Biosensors 2017, 7, 46.
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