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Biosensors, Volume 11, Issue 3

March 2021 - 37 articles

Cover Story: High-yield protein production of the Aryl hydrocarbon Receptor (AhR) by bacterial expression occurs in the presence of the AhR Nuclear Translocator (ARNT). Protein complexes are purified by a combination of affinity and size exclusion chromatography (Fast Protein Liquid Chromatography, FPLC). For Microscale Thermophoresis (MST) measurements, a serial dilution of non-fluorescent ligand is titrated to purified AhR–ARNT complexes and loaded on MST capillaries. Upon laser exposure, thermophoresis- and temperature-induced changes in the intrinsic fluorescence of the protein complex (AhR–ARNT) are determined, and ligand-binding constants are calculated. View this paper.
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Articles (37)

  • Article
  • Open Access
41 Citations
4,884 Views
11 Pages

23 March 2021

We report a liquid crystal (LC)-based aptasensor for the detection of malathion using a cationic surfactant-decorated LC interface. In this method, LCs displayed dark optical images when in contact with aqueous cetyltrimethylammonium bromide (CTAB) s...

  • Article
  • Open Access
24 Citations
5,524 Views
22 Pages

23 March 2021

The technological advantages that biosensors have over conventional technical sensors for odor detection and the role they play in the biological transformation have not yet been comprehensively analyzed. However, this is necessary for assessing thei...

  • Article
  • Open Access
6 Citations
4,288 Views
16 Pages

Multi-Layer Reflectivity Calculation Based Meta-Modeling of the Phase Mapping Function for Highly Reproducible Surface Plasmon Resonance Biosensing

  • Tzu-Heng Wu,
  • Ching-Hsu Yang,
  • Chia-Chen Chang,
  • Hui-Wen Liu,
  • Chia-Yu Yang,
  • Tang-Long Shen,
  • Chii-Wann Lin and
  • Aurélien Bruyant

23 March 2021

Phase-sensitive surface plasmon resonance biosensors are known for their high sensitivity. One of the technology bottle-necks of such sensors is that the phase sensorgram, when measured at fixed angle set-up, can lead to low reproducibility as the si...

  • Review
  • Open Access
52 Citations
7,946 Views
42 Pages

23 March 2021

Cytokines are soluble proteins secreted by immune cells that act as molecular messengers relaying instructions and mediating various functions performed by the cellular counterparts of the immune system, by means of a synchronized cascade of signalin...

  • Article
  • Open Access
21 Citations
5,993 Views
17 Pages

A Simple Distance Paper-Based Analytical Device for the Screening of Lead in Food Matrices

  • Kasinee Katelakha,
  • Vanida Nopponpunth,
  • Watcharee Boonlue and
  • Wanida Laiwattanapaisal

22 March 2021

A simple and rapid distance paper-based analytical device (dPAD) for the detection of lead (Pb) in foods is proposed herein. The assay principle is based on competitive binding between carminic acid (CA) and polyethyleneimine (PEI) to Pb in a food sa...

  • Article
  • Open Access
11 Citations
3,489 Views
14 Pages

Enzymatic Electroanalytical Biosensor Based on Maramiellus colocasiae Fungus for Detection of Phytomarkers in Infusions and Green Tea Kombucha

  • Érica A. Batista,
  • Giovanna N. M. Silva,
  • Livia F. Sgobbi,
  • Fabio B. Machado,
  • Isaac Y. Macedo,
  • Emily K. Moreno,
  • Jerônimo R. Neto,
  • Paulo S. Scalize and
  • Eric S. Gil

22 March 2021

In this work, we developed an enzymatic voltammetric biosensor for the determination of catechin and gallic acid in green tea and kombucha samples. The differential pulse voltammetry (DPV) methodology was optimized regarding the amount of crude enzym...

  • Review
  • Open Access
33 Citations
5,116 Views
17 Pages

21 March 2021

The molecular imprinting polymers (MIPs) have shown their potential in various applications including pharmaceuticals, chemical sensing and biosensing, medical diagnosis, and environmental related issues, owing to their artificial selective biomimeti...

  • Article
  • Open Access
25 Citations
4,623 Views
13 Pages

19 March 2021

Ochratoxin A (OTA) primarily obtained by the genera aspergillus and penicillium, is one of the toxic substances for different organs and systems of the human body such as the kidney, liver, neurons and the immune system. Moreover, it is considered to...

  • Article
  • Open Access
57 Citations
8,228 Views
9 Pages

Specific Detection of Influenza A and B Viruses by CRISPR-Cas12a-Based Assay

  • Bum Ju Park,
  • Man Seong Park,
  • Jae Myun Lee and
  • Yoon Jae Song

19 March 2021

A rapid and accurate on-site diagnostic test for pathogens including influenza viruses is critical for preventing the spread of infectious diseases. Two types of influenza virus, A and B cause seasonal flu epidemics, whereas type A can cause influenz...

  • Article
  • Open Access
16 Citations
4,661 Views
12 Pages

18 March 2021

Many studies concern the generation of lossy mode resonances (LMRs) using metallic oxide thin films that are deposited on optical fiber. However, the LMR-based optical fiber sensors are frangible, do not allow easy surface modification, and are not s...

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Biosensors - ISSN 2079-6374