Biosensors, Volume 11, Issue 2 (February 2021) – 32 articles
Cover Story (view full-size image): The measurement of the chemical parameters of binding between cell adhesion molecules and extracellu-lar ligands using live-cell assays is a challenging task. Here, for the first time, we used a computer-controlled micropipette to measure cell adhesion on PLL-g-PEG-based surface coatings with various ligand densities to determine the dissociation constant of the integrin–RGD bond. We use a simple biophysical model to fit the weighted average of adhesion histograms. The results are confirmed by applying a resonant waveguide-based biosensor. Furthermore, we present a subpopulation analysis of the data emphasizing the single-cell capabili-ties of the method. We find that most cells are found in the weak adhesion range, while a growing, strongly adhered subpopulation appears with the increase of RGD surface density. View this paper.
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