Biosensors, Volume 11, Issue 4 (April 2021) – 32 articles
Cover Story (view full-size image): Selenium (Se) is an essential micronutrient which has antioxidant properties and is known to play a role in cancer prevention. Green algae Chlorella vulgaris cultivated in Se-enriched environment may be used as a food supplement to provide adequate levels of Se. We used Raman microspectroscopy (RS) for fast and nondestructive measurement of Se concentration in algal cells. We employed inductively coupled plasma mass spectrometry as a reference method for RS and found a solid correlation between the Raman signal intensity at 252 cm−1 and total Se concentration in the studied cells. We used RS to demonstrated the necessity of active cellular transport for Se accumulation. Additionally, we observed the intracellular Se being transformed into an elemental form, which we further supported using energy-dispersive X-ray spectroscopy imaging. View this paper.
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