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Microorganisms, Volume 10, Issue 2

2022 February - 288 articles

Cover Story: Phototrophic mixed cultures (PMC) are versatile systems which can be applied for waste streams valorisation and production of added-value compounds, such as polyhydroxyalkanoates (PHA). This work employed DNA sequencing, fluorescence in situ hybridization (FISH), Nile blue staining, and morphological observations to deepen our current knowledge of the bacterial communities present in PHA-producing PMC systems. Links between microbial communities and their PHA production performance were established and microbial groups with high PHA accumulation capacity were identified, namely, Chromatiaceae members. Knowledge of the operating conditions that maximize the presence of such groups and PHA storage will help to define improved operational strategies for PHA production with PMC systems. View this paper
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Articles (288)

  • Article
  • Open Access
16 Citations
4,872 Views
22 Pages

Cupriavidus metallidurans CH34 Possesses Aromatic Catabolic Versatility and Degrades Benzene in the Presence of Mercury and Cadmium

  • Pablo Alviz-Gazitua,
  • Roberto E. Durán,
  • Felipe A. Millacura,
  • Franco Cárdenas,
  • Luis A. Rojas and
  • Michael Seeger

Heavy metal co-contamination in crude oil-polluted environments may inhibit microbial bioremediation of hydrocarbons. The model heavy metal-resistant bacterium Cupriavidus metallidurans CH34 possesses cadmium and mercury resistance, as well as genes...

  • Review
  • Open Access
8 Citations
3,872 Views
13 Pages

Listeria monocytogenes is a foodborne pathogen that typically presents β-hemolytic activity. However, there are literature reports indicating that L. monocytogenes strains are sometimes nonhemolytic or their zones of hemolysis are perceivable on...

  • Article
  • Open Access
11 Citations
3,426 Views
13 Pages

Organic and Inorganic Amendments Shape Bacterial Indicator Communities That Can, In Turn, Promote Rice Yield

  • Chongwen Qiu,
  • Yuanyuan Bao,
  • Evangelos Petropoulos,
  • Yiming Wang,
  • Zhenfang Zhong,
  • Yaozhi Jiang,
  • Xuhong Ye,
  • Xiangui Lin and
  • Youzhi Feng

The dynamic patterns of the belowground microbial communities and their corresponding metabolic functions, when exposed to various environmental disturbances, are important for the understanding and development of sustainable agricultural systems. In...

  • Article
  • Open Access
8 Citations
3,659 Views
23 Pages

Metagenomic Assessment of DNA Viral Diversity in Freshwater Sponges, Baikalospongia bacillifera

  • Tatyana V. Butina,
  • Ivan S. Petrushin,
  • Igor V. Khanaev and
  • Yurij S. Bukin

Sponges (type Porifera) are multicellular organisms that give shelter to a variety of microorganisms: fungi, algae, archaea, bacteria, and viruses. The studies concerning the composition of viral communities in sponges have appeared rather recently,...

  • Article
  • Open Access
11 Citations
3,476 Views
15 Pages

Thermokarst lakes are important aquatic ecosystems in cold regions, experiencing several changes due to global warming. However, the fundamental assembly mechanisms of microeukaryotic communities in thermokarst lakes are unknown. In this study, we ex...

  • Article
  • Open Access
8 Citations
3,463 Views
14 Pages

Shallow-Water Hydrothermal Vents as Natural Accelerators of Bacterial Antibiotic Resistance in Marine Coastal Areas

  • Erika Arcadi,
  • Eugenio Rastelli,
  • Michael Tangherlini,
  • Carmen Rizzo,
  • Monique Mancuso,
  • Marilena Sanfilippo,
  • Valentina Esposito,
  • Franco Andaloro and
  • Teresa Romeo

Environmental contamination by heavy metals (HMs) poses several indirect risks to human health, including the co-spreading of genetic traits conferring resistance to both HMs and antibiotics among micro-organisms. Microbial antibiotic resistance (AR)...

  • Communication
  • Open Access
4 Citations
3,057 Views
8 Pages

Emergence of Novel Chlamydia trachomatis Sequence Types among Chlamydia Patients in the Republic of Belarus

  • Valentina A. Feodorova,
  • Yury V. Saltykov,
  • Anna A. Kolosova,
  • Liudmila V. Rubanik,
  • Nikolay N. Poleshchuk and
  • Vladimir L. Motin

Chlamydia trachomatis (CT) is a major cause of sexually transmitted diseases worldwide. The multilocus sequence typing (MLST) of clinical samples from random heterosexual chlamydia patients who were either asymptomatic or reported clinical manifestat...

  • Technical Note
  • Open Access
10 Citations
3,733 Views
8 Pages

Macroalgae-associated bacteria play an important role in their algal hosts’ biological processes. They are localized on surfaces of the host thalli, as well as between and even within algal cells. To examine the differences in community structu...

  • Article
  • Open Access
26 Citations
5,300 Views
16 Pages

Identification of a Novel Biosurfactant with Antimicrobial Activity Produced by Rhodococcus opacus R7

  • Jessica Zampolli,
  • Alessandra De Giani,
  • Alessandra Di Canito,
  • Guido Sello and
  • Patrizia Di Gennaro

Rhodococcus members excrete secondary metabolites, especially compounds which act as biosurfactants. In this work, we demonstrated the ability of Rhodococcus opacus R7 to produce a novel bioactive compound belonging to the class of biosurfactants wit...

  • Article
  • Open Access
18 Citations
7,854 Views
15 Pages

Urinary Tract Infections and Associated Factors among Patients with Indwelling Urinary Catheters Attending Bugando Medical Centre a Tertiary Hospital in Northwestern Tanzania

  • Asteria L. M. Ndomba,
  • Rose M. Laisser,
  • Vitus Silago,
  • Benson R. Kidenya,
  • Joseph Mwanga,
  • Jeremiah Seni and
  • Stephen E. Mshana

Complications of indwelling urinary catheterization (IUC) are associated with significant morbidity and mortality, thus affecting patient’s well-being. Understanding the magnitude and factors associated with complications is crucial in designin...

  • Article
  • Open Access
5 Citations
3,410 Views
8 Pages

A rapid and label-free method for the detection of drug-resistant pathogens is in high demand for wastewater-based epidemiology. As recently shown, the extent of electrical current production (Ic) is a useful indicator of a pathogen’s metabolic...

  • Article
  • Open Access
19 Citations
5,402 Views
14 Pages

The present study investigated the anti-bacterial vaginitis (BV) effects of a mixture of five lactobacilli strains (LM5), containing equal amounts of Ligilactobacillus salivarius MG242, Limosilactobacillus fermentum MG901, Lactiplantibacillus plantar...

  • Article
  • Open Access
28 Citations
5,316 Views
16 Pages

The Combination of In Vitro Assessment of Stress Tolerance Ability, Autoaggregation, and Vitamin B-Producing Ability for New Probiotic Strain Introduction

  • Natalya Yu. Khromova,
  • Julia M. Epishkina,
  • Boris A. Karetkin,
  • Natalia V. Khabibulina,
  • Andrey V. Beloded,
  • Irina V. Shakir and
  • Victor I. Panfilov

The health benefits of probiotics are beyond doubt. The positive effects of lactobacilli and bifidobacteria on the function of many body systems have been repeatedly proven by various studies. To completely realize the potential of probiotic microorg...

  • Article
  • Open Access
6 Citations
3,149 Views
13 Pages

Molecular Epidemiology of Multidrug-Resistant Pneumococci among Ghanaian Children under Five Years Post PCV13 Using MLST

  • Richael O. Mills,
  • Mohammed R. Abdullah,
  • Samuel A. Akwetey,
  • Dorcas C. Sappor,
  • Gustavo Gámez and
  • Sven Hammerschmidt

Antibiotic resistance in pneumococci contributes to the high pneumococcal deaths in children. We assessed the molecular characteristics of multidrug-resistant (MDR) pneumococci isolated from healthy vaccinated children under five years of age in Cape...

  • Review
  • Open Access
34 Citations
6,084 Views
11 Pages

Bacterial biofilms contribute significantly to the antibiotic resistance, pathogenesis, chronicity and recurrence of bacterial infections. Critical to the stability and survival of extant biofilms is the extracellular DNA (eDNA)-dependent matrix whic...

  • Article
  • Open Access
9 Citations
3,631 Views
19 Pages

A Parallel Tracking of Salivary and Gut Microbiota Profiles Can Reveal Maturation and Interplay of Early Life Microbial Communities in Healthy Infants

  • Sofia Reddel,
  • Giuseppe Rubens Pascucci,
  • Silvia Foligno,
  • Federica Del Chierico,
  • Pamela Vernocchi,
  • Alessandra Marzullo,
  • Maria Grazia Pattumelli,
  • Paolo Palma,
  • Guglielmo Salvatori and
  • Lorenza Putignani

In this study, the onset and shaping of the salivary and gut microbiota in healthy newborns during the first period of life has been followed, evaluating the impact of salivary microbiota on the development of early fecal microbial communities. The m...

  • Article
  • Open Access
15 Citations
4,809 Views
19 Pages

Here, we report a first comprehensive genomic analysis of SARS-CoV-2 variants circulating in North African countries, including Algeria, Egypt, Libya, Morocco, Sudan and Tunisia, with respect to genomic clades and mutational patterns. As of December...

  • Article
  • Open Access
7 Citations
3,689 Views
20 Pages

From Rest to Growth: Life Collisions of Gordonia polyisoprenivorans 135

  • Nataliya E. Suzina,
  • Vladimir V. Sorokin,
  • Valentina N. Polivtseva,
  • Violetta V. Klyueva,
  • Elena V. Emelyanova and
  • Inna P. Solyanikova

In the process of evolution, living organisms develop mechanisms for population preservation to survive in unfavorable conditions. Spores and cysts are the most obvious examples of dormant forms in microorganisms. Non-spore-forming bacteria are also...

  • Review
  • Open Access
16 Citations
8,600 Views
17 Pages

Vaccination against Tick-Borne Encephalitis (TBE) in Italy: Still a Long Way to Go

  • Donatella Panatto,
  • Alexander Domnich,
  • Daniela Amicizia,
  • Paolo Reggio and
  • Raffaella Iantomasi

Tick-borne encephalitis (TBE) is endemic in several European countries, and its incidence has recently increased. Various factors may explain this phenomenon: social factors (changes in human behavior, duration and type of leisure activities and incr...

  • Article
  • Open Access
43 Citations
5,067 Views
12 Pages

Probiotic Potentiality from Versatile Lactiplantibacillus plantarum Strains as Resource to Enhance Freshwater Fish Health

  • Massimo Iorizzo,
  • Gianluca Albanese,
  • Francesco Letizia,
  • Bruno Testa,
  • Patrizio Tremonte,
  • Franca Vergalito,
  • Silvia Jane Lombardi,
  • Mariantonietta Succi,
  • Raffaele Coppola and
  • Elena Sorrentino

Dietary probiotic supplementation has the potential to enhance the health of fish and their disease resistance. In this study, some properties of ten Lactiplantibacillus plantarum strains have been evaluated, for their potential use as probiotics in...

  • Article
  • Open Access
73 Citations
6,462 Views
19 Pages

Long-Term Application of Bio-Compost Increased Soil Microbial Community Diversity and Altered Its Composition and Network

  • Xiayan Liu,
  • Yu Shi,
  • Lingyu Kong,
  • Lihong Tong,
  • Haoxuan Cao,
  • Hu Zhou and
  • Yizhong Lv

The influence of bio-compost on the diversity, composition and structure of soil microbial communities is less understood. Here, Illumina MiSeq sequencing and a network analysis were used to comprehensively characterize the effects of 25 years of bio...

  • Article
  • Open Access
7 Citations
5,112 Views
14 Pages

Molecular Typing of Ukrainian Bacillus anthracis Strains by Combining Whole-Genome Sequencing Techniques

  • Hanka Brangsch,
  • Anatolii Golovko,
  • Nataliia Pinchuk,
  • Oleg Deriabin,
  • Tetiana Kyselova,
  • Jörg Linde,
  • Falk Melzer and
  • Mandy Carolina Elschner

Anthrax is a recurrent zoonosis in the Ukraine with outbreaks occurring repeatedly in certain areas. For determining whether several Bacillus anthracis genotypes are circulating in this region, four strains from various sources isolated from differen...

  • Article
  • Open Access
6 Citations
3,829 Views
14 Pages

Culture-Free Enumeration of Mycobacterium tuberculosis in Mouse Tissues Using the Molecular Bacterial Load Assay for Preclinical Drug Development

  • Dimitrios Evangelopoulos,
  • Carolyn M. Shoen,
  • Isobella Honeyborne,
  • Simon Clark,
  • Ann Williams,
  • Galina V. Mukamolova,
  • Michael H. Cynamon and
  • Timothy D. McHugh

Background: The turnaround times for phenotypic tests used to monitor the bacterial load of Mycobacterium tuberculosis, in both clinical and preclinical studies, are delayed by the organism’s slow growth in culture media. The existence of diffe...

  • Article
  • Open Access
2 Citations
3,803 Views
13 Pages

The biodegradable polyester poly-(R)-3-hydroxybutyrate [P(3HB)] is synthesized by a polymerizing enzyme called polyhydroxyalkanoate (PHA) synthase and accumulates in a wide variety of bacterial cells. Recently, we demonstrated the secretory productio...

  • Article
  • Open Access
5 Citations
4,698 Views
20 Pages

Few data exist on the human gut mycobiome in relation to lifestyle, ethnicity, and dietary habits. To understand the effect of these factors on the structure of the human gut mycobiome, we analyzed sequences belonging to two extinct pre-Columbian cul...

  • Article
  • Open Access
5 Citations
4,420 Views
19 Pages

Bermudagrass Cultivars with Different Tolerance to Nematode Damage Are Characterized by Distinct Fungal but Similar Bacterial and Archaeal Microbiomes

  • Chang Jae Choi,
  • Jacqueline Valiente,
  • Marco Schiavon,
  • Braham Dhillon,
  • William T. Crow and
  • Ulrich Stingl

Turfgrass landscapes have expanded rapidly in recent decades and are a major vegetation type in urbanizing ecosystems. While turfgrass areas provide numerous ecosystem services in urban environments, ecological side effects from intensive management...

  • Article
  • Open Access
8 Citations
3,338 Views
14 Pages

Combination of High-Pressure Treatment at 500 MPa and Biopreservation with a Lactococcus lactis Strain for Lowering the Bacterial Growth during Storage of Diced Cooked Ham with Reduced Nitrite Salt

  • Stéphane Chaillou,
  • Mihanta Ramaroson,
  • Gwendoline Coeuret,
  • Albert Rossero,
  • Valérie Anthoine,
  • Marie Champomier-Vergès,
  • Nicolas Moriceau,
  • Sandrine Rezé,
  • Jean-Luc Martin and
  • Monique Zagorec
  • + 1 author

We investigated the combined effects of biopreservation and high-pressure treatment on bacterial communities of diced cooked ham prepared with diminished nitrite salt. First, bacterial communities of four commercial brands of diced cooked ham from lo...

  • Article
  • Open Access
13 Citations
4,872 Views
20 Pages

We survey here the Alphaproteobacteria, a large class encompassing physiologically diverse bacteria which are divided in several orders established since 2007. Currently, there is considerable uncertainty regarding the classification of an increasing...

  • Review
  • Open Access
11 Citations
6,110 Views
21 Pages

Mycobacterial Adhesion: From Hydrophobic to Receptor-Ligand Interactions

  • Albertus Viljoen,
  • Yves F. Dufrêne and
  • Jérôme Nigou

Adhesion is crucial for the infective lifestyles of bacterial pathogens. Adhesion to non-living surfaces, other microbial cells, and components of the biofilm extracellular matrix are crucial for biofilm formation and integrity, plus adherence to hos...

  • Review
  • Open Access
81 Citations
10,972 Views
19 Pages

Actinomycetes from Caves: An Overview of Their Diversity, Biotechnological Properties, and Insights for Their Use in Soil Environments

  • Beatrice Farda,
  • Rihab Djebaili,
  • Ilaria Vaccarelli,
  • Maddalena Del Gallo and
  • Marika Pellegrini

The environmental conditions of caves shape microbiota. Within caves’ microbial communities, actinomycetes are among the most abundant bacteria. Cave actinomycetes have gained increasing attention during the last decades due to novel bioactive...

  • Review
  • Open Access
128 Citations
11,835 Views
15 Pages

Obesity, a worldwide health concern with a constantly rising prevalence, is a multifactorial chronic disease associated with a wide range of physiological disruptions, including energy imbalance, central appetite and food reward dysregulation, and ho...

  • Review
  • Open Access
42 Citations
8,640 Views
7 Pages

Literature Review of Omicron: A Grim Reality Amidst COVID-19

  • Suraj Arora,
  • Vishakha Grover,
  • Priyanka Saluja,
  • Youssef Abdullah Algarni,
  • Shahabe Abullais Saquib,
  • Shaik Mohammed Asif,
  • Kavita Batra,
  • Mohammed Y. Alshahrani,
  • Gotam Das and
  • Anchal Ohri
  • + 1 author

Coronavirus disease 2019 (COVID-19) first emerged in Wuhan city in December 2019, and became a grave global concern due to its highly infectious nature. The Severe Acute Respiratory Coronavirus-2, with its predecessors (i.e., MERS-CoV and SARS-CoV) b...

  • Article
  • Open Access
13 Citations
4,370 Views
14 Pages

The Leaf Microbiome of Tobacco Plants across Eight Chinese Provinces

  • Haiyang Hu,
  • Yunli Liu,
  • Yiqun Huang,
  • Zhan Zhang and
  • Hongzhi Tang

Leaf microorganism communities play significant roles in the process of plant growth, but the microbiome profiling of crop leaves is still a relatively new research area. Here, we used 16S rDNA sequencing to profile the microbiomes of 78 primary drie...

  • Article
  • Open Access
10 Citations
3,610 Views
12 Pages

Genetic Characterization of Multidrug-Resistant E. coli Isolates from Bloodstream Infections in Lithuania

  • Tatjana Kirtikliene,
  • Aistė Mierauskaitė,
  • Ilona Razmienė and
  • Nomeda Kuisiene

Extraintestinal pathogenic Escherichia coli (ExPEC) isolates are a main cause of bloodstream infections. The aim of this study was to characterize 256 β-lactam–resistant, bacteremia-causing E. coli isolates collected from 12 healthcare ins...

  • Article
  • Open Access
1 Citations
3,610 Views
14 Pages

Reduction of Bacterial Enteric Pathogens and Hygiene Indicator Bacteria on Tomato Skin Surfaces by a Polymeric Nanoparticle-Loaded Plant-Derived Antimicrobial

  • Keila L. Perez-Lewis,
  • Yagmur Yegin,
  • Jun K. Oh,
  • Alejandro Castillo,
  • Luis Cisneros-Zevallos,
  • Chris R. Kerth,
  • Mustafa Akbulut and
  • Thomas M. Taylor

This study determined Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium survival on tomato skins as a function of sanitization treatment, under three differing contamination and sanitization scenarios. Sanitizing treatments consist...

  • Article
  • Open Access
7 Citations
3,416 Views
15 Pages

Genotyping Canadian Cyclospora cayetanensis Isolates to Supplement Cyclosporiasis Outbreak Investigations

  • Christine A. Yanta,
  • John R. Barta,
  • Antoine Corbeil,
  • Hervé Menan,
  • Karine Thivierge,
  • Robert Needle,
  • Muhammad Morshed,
  • Brent R. Dixon,
  • James D. Wasmuth and
  • Rebecca A. Guy

Cyclospora cayetanensis is an emerging foodborne parasite that causes cyclosporiasis, an enteric disease of humans. Domestically acquired outbreaks have been reported in Canada every spring or summer since 2013. To date, investigations into the poten...

  • Article
  • Open Access
10 Citations
3,466 Views
20 Pages

Snf1 Kinase Differentially Regulates Botrytis cinerea Pathogenicity according to the Plant Host

  • Szabina Lengyel,
  • Christine Rascle,
  • Nathalie Poussereau,
  • Christophe Bruel,
  • Luca Sella,
  • Mathias Choquer and
  • Francesco Favaron

The Snf1 kinase of the glucose signaling pathway controls the response to nutritional and environmental stresses. In phytopathogenic fungi, Snf1 acts as a global activator of plant cell wall degrading enzymes that are major virulence factors for plan...

  • Article
  • Open Access
18 Citations
3,905 Views
19 Pages

Detection, Molecular Identification and Transmission of the Intestinal Protozoa Blastocystis sp. in Guinea from a Large-Scale Epidemiological Study Conducted in the Conakry Area

  • Timothé Guilavogui,
  • Nausicaa Gantois,
  • Gaël Even,
  • Jeremy Desramaut,
  • Ellena Dautel,
  • Constance Denoyelle,
  • Fode Ibrahima Cissé,
  • Salif Cherif Touré,
  • Bakary Luther Kourouma and
  • Eric Viscogliosi
  • + 3 authors

Blastocystis sp. is a single-celled parasite estimated to colonize the digestive tract of 1 to 2 billion people worldwide. Although it represents the most frequent intestinal protozoa in human stools, it remains still under-investigated in countries...

  • Article
  • Open Access
6 Citations
3,233 Views
12 Pages

Drug alternatives to combat methicillin-resistant Staphylococcus aureus (MRSA) in human and animal healthcare are urgently needed. Recently, the recombinant bacteriophage endolysins, PRF-119 and its successor substance HY-133, have proven to be highl...

  • Article
  • Open Access
18 Citations
4,478 Views
17 Pages

Intermittent reduction of temperature set-points and periodic shutdowns of water heaters have been proposed to reduce energy consumption in buildings. However, the consequences of such measures on the occurrence and proliferation of Legionella pneumo...

  • Review
  • Open Access
23 Citations
7,350 Views
17 Pages

Adaptation to the changing environmental conditions experienced within a host requires genetic diversity within a microbial population. Genetic diversity arises from mutations which occur due to DNA damage from exposure to exogenous environmental str...

  • Article
  • Open Access
15 Citations
6,372 Views
19 Pages

Clinical Metagenomics Is Increasingly Accurate and Affordable to Detect Enteric Bacterial Pathogens in Stool

  • Christy-Lynn Peterson,
  • David Alexander,
  • Julie Chih-Yu Chen,
  • Heather Adam,
  • Matthew Walker,
  • Jennifer Ali,
  • Jessica Forbes,
  • Eduardo Taboada,
  • Dillon O. R. Barker and
  • Aleisha R. Reimer
  • + 2 authors

Stool culture is the gold standard method to diagnose enteric bacterial infections; however, many clinical laboratories are transitioning to syndromic multiplex PCR panels. PCR is rapid, accurate, and affordable, yet does not yield subtyping informat...

  • Article
  • Open Access
9 Citations
3,315 Views
15 Pages

In nature, most plants parasitized by root hemiparasites are also colonized by mutualistic arbuscular mycorrhizal (AM) fungi, highlighting the prevalence of this tripartite interaction. AM colonization is generally found to improve the growth of para...

  • Article
  • Open Access
16 Citations
4,275 Views
14 Pages

Bacterial Biofilm Formation on Nano-Copper Added PLA Suited for 3D Printed Face Masks

  • Annika Kiel,
  • Bernhard Peter Kaltschmidt,
  • Ehsan Asghari,
  • Andreas Hütten,
  • Barbara Kaltschmidt and
  • Christian Kaltschmidt

The COVID-19 Pandemic leads to an increased worldwide demand for personal protection equipment in the medical field, such as face masks. New approaches to satisfy this demand have been developed, and one example is the use of 3D printing face masks....

  • Article
  • Open Access
30 Citations
3,921 Views
17 Pages

Transcriptomic Analysis of Degradative Pathways for Azo Dye Acid Blue 113 in Sphingomonas melonis B-2 from the Dye Wastewater Treatment Process

  • Aalfin-Emmanuel Santhanarajan,
  • Chaeyoung Rhee,
  • Woo Jun Sul,
  • Keunje Yoo,
  • Hoon Je Seong,
  • Hong-Gi Kim and
  • Sung-Cheol Koh

Background: Acid Blue 113 (AB113) is a typical azo dye, and the resulting wastewater is toxic and difficult to remove. Methods: The experimental culture was set up for the biodegradation of the azo dye AB113, and the cell growth and dye decolorizatio...

  • Review
  • Open Access
175 Citations
10,355 Views
20 Pages

Current Knowledge on the Oxidative-Stress-Mediated Antimicrobial Properties of Metal-Based Nanoparticles

  • Nour Mammari,
  • Emmanuel Lamouroux,
  • Ariane Boudier and
  • Raphaël E. Duval

The emergence of multidrug-resistant (MDR) bacteria in recent years has been alarming and represents a major public health problem. The development of effective antimicrobial agents remains a key challenge. Nanotechnologies have provided opportunitie...

  • Article
  • Open Access
30 Citations
4,253 Views
17 Pages

Biochar has been widely recognized as an effective and eco-friendly ameliorant for saline soils, but information about the mechanism of how biochar influences nitrification in salt-affected agroecosystem remains fragmented. An incubation experiment w...

  • Article
  • Open Access
15 Citations
5,918 Views
13 Pages

MALDI-TOF MS Using a Custom-Made Database, Biomarker Assignment, or Mathematical Classifiers Does Not Differentiate Shigella spp. and Escherichia coli

  • Maaike J. C. van den Beld,
  • John W. A. Rossen,
  • Noah Evers,
  • Mirjam A. M. D. Kooistra-Smid and
  • Frans A. G. Reubsaet

Shigella spp. and E. coli are closely related and cannot be distinguished using matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS) with commercially available databases. Here, three alternative approaches usin...

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Microorganisms - ISSN 2076-2607