Table of Contents
Crystals, Volume 9, Issue 9 (September 2019) – 51 articles
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Cover Story (view full-size image) The crystallization of glulisine was analyzed through its crystallization phase diagram and the use [...] Read more. The crystallization of glulisine was analyzed through its crystallization phase diagram and the use of nucleation-inducing materials. We have shown that the same protein can have different solubility behaviors depending on the nature of the salt in the precipitating agent. In the case of magnesium formate, lowering the precipitant concentration drove the system further into supersaturation, resulting in the formation of crystals and precipitation. This effect is opposite to the usual scenario, where raising the precipitant concentration leads to supersaturation. When using sodium potassium tartrate tetrahydrate, we observed the expected trend of crystal formation or precipitation at higher concentrations, and clear drops at lower concentrations of precipitant. Glulisine was successfully crystallized, producing crystals diffracting up to a resolution limit of 1.4 Å. View this paper