Analysis of Glulisine Crystallisation Utilising Phase Diagrams and Nucleants
Abstract
:1. Introduction
1.1. The Application of the Phase Diagram and Nucleants
1.2. Glulisine
2. Materials and Methods
2.1. Protein and Precipitants
2.2. Crystallisation Methods
2.3. Nucleants
2.4. Crystal Harvesting and Data Collection
3. Results
3.1. Glulisine and Magnesium Formate
3.2. Glulisine and Sodium Potassium Tartrate Tetrahydrate (NaKT)
4. Discussion
4.1. The Solubility of Glulisine
4.2. Experimental and Technical Evaluations
4.3. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Li, Y.; Govada, L.; Solomon, H.V.; Gillis, R.B.; Adams, G.G.; Chayen, N.E. Analysis of Glulisine Crystallisation Utilising Phase Diagrams and Nucleants. Crystals 2019, 9, 462. https://doi.org/10.3390/cryst9090462
Li Y, Govada L, Solomon HV, Gillis RB, Adams GG, Chayen NE. Analysis of Glulisine Crystallisation Utilising Phase Diagrams and Nucleants. Crystals. 2019; 9(9):462. https://doi.org/10.3390/cryst9090462
Chicago/Turabian StyleLi, Yanmin, Lata Govada, Hodaya V. Solomon, Richard B. Gillis, Gary G. Adams, and Naomi E. Chayen. 2019. "Analysis of Glulisine Crystallisation Utilising Phase Diagrams and Nucleants" Crystals 9, no. 9: 462. https://doi.org/10.3390/cryst9090462