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Microorganisms
Special Issues
Selected Papers from the 3rd International Electronic Conference on Microbiology...
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Selected Papers from the 3rd International Electronic Conference on Microbiology (ECM 2025)

A special issue of Microorganisms (ISSN 2076-2607).

Deadline for manuscript submissions: 31 December 2025 | Viewed by 1022

Special Issue Editors

Dr. Nico Jehmlich

E-Mail Website
Guest Editor
Department of Molecular Toxicology, UFZ-Helmholtz Centre for Environmental Research, 04318 Leipzig, Germany
Interests: metaproteomics; protein-stable isotope probing; microbiome biology; environmental microbiology
Special Issues, Collections and Topics in MDPI journals
Prof. Dr. Theodoros Varzakas

E-Mail Website
Guest Editor
Department of Food Science and Technology, University of Peloponnese, 24100 Antikalamos, Greece
Interests: food technology; food engineering; food safety; food quality; extra virgin olive oil; mycotoxins; fermented foods
Special Issues, Collections and Topics in MDPI journals
Prof. Dr. Ute Römling

E-Mail Website
Guest Editor
Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet, Stockholm, Sweden
Interests: microbial biofilm formation; cyclic di-nucleotide signaling; pathogen-host interaction; protein quality control; Salmonella typhimurium; Pseudomonas aeruginosa; Candida parapsilosis
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

This Special Issue, titled "Selected Papers from the 3rd International Electronic Conference on Microbiology (ECM 2025)", will present the latest research on prokaryotic and eukaryotic microorganisms, viruses, and prions. Topics of interest include, but are not limited to, the following:

  • S1. Gut Microbiota and Health Disease;
  • S2. Foodborne Pathogens and Food Safety;
  • S3. Antimicrobial Agents and Resistance;
  • S4. Emerging Infectious Diseases;
  • S5. Microbiome and Soil Science;
  • S6. Microbial Characterization and Bioprocess
  • S7. Microbe-Plant Interactions

We are cooperating with the 3rd International Electronic Conference on Microbiology (ECM 2025). Registered conference participants are invited to submit their manuscripts to be considered for publication. Authors may contribute an original research article or review in areas related to the conference themes.

Dr. Nico Jehmlich
Prof. Dr. Theodoros Varzakas
Prof. Dr. Ute Römling
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Microorganisms is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • gut microbiota
  • foodborne pathogens and food safety
  • antimicrobial agents and resistance
  • emerging infectious diseases
  • microbiome and soil science
  • microbial characterization and bioprocess
  • microbe-plant interactions

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Further information on MDPI's Special Issue policies can be found here.

Published Papers (1 paper)

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31 pages, 1208 KiB  
Systematic Review
Exploring Methodologies from Isolation to Excystation for Giardia lamblia: A Systematic Review
by Susie Sequeira, Mariana Sousa and Agostinho Cruz
Microorganisms 2025, 13(8), 1719; https://doi.org/10.3390/microorganisms13081719 - 22 Jul 2025
Viewed by 404
Abstract
Giardia lamblia is a flagellated protozoan and the etiological agent of giardiasis, a leading cause of epidemic and sporadic diarrhoea globally. The clinical and public health relevance of giardiasis underscores the need for robust methodologies to investigate and manage this pathogen. This study [...] Read more.
Giardia lamblia is a flagellated protozoan and the etiological agent of giardiasis, a leading cause of epidemic and sporadic diarrhoea globally. The clinical and public health relevance of giardiasis underscores the need for robust methodologies to investigate and manage this pathogen. This study reviews the main methodologies described in the literature for studying the life cycle of G. lamblia, focusing on isolation, purification, axenization, excystation, and encystation. A systematic literature review was conducted following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA 2020) statement. Searches were performed in MEDLINE, ScienceDirect, and Web of Science Core Collection databases. A total of 43 studies were included, revealing 58 methods for isolation and purification, 7 for excystation, 2 for axenization, and 5 for encystation. Isolation and purification methods exhibited significant variability, often involving two phases: an initial separation (e.g., filtration and centrifugation) followed by purification using a density gradient for faecal samples or immunomagnetic separation for water samples. Method effectiveness differed depending on the sample source and type, limiting comparability across studies. In contrast, methods used for other life cycle stages were more consistent. These findings underscore the need for standardised methodologies to enhance the reproducibility and reliability of research outcomes in this field. Full article
(This article belongs to the Special Issue Selected Papers from the 3rd International Electronic Conference on Microbiology (ECM 2025))
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