Bacteria

Bacteria adapted to elevated temperatures are commonly associated with geothermal environments and are recognized for their functional diversity. In this study, cultivable bacteria were isolated from a geothermal spring in northern Sinaloa, Mexico, and characterized through physicochemical analysis, molecular identification, growth kinetics, and functional screening. The isolates were identified as Bacillus licheniformis (strains J1, J3, and J8) and Brevibacillus borstelensis (strains J6 and J9). Growth analyses showed that, in nutrient broth at 45 °C, the evaluated strains exhibited specific growth rates ranging from 1.25 to 1.78 h⁻¹ and short doubling times between 23 and 33 min, with B. borstelensis J6 displaying the highest rate. At 50 °C, μmax values ranged from 0.77 to 1.08 h⁻¹, indicating sustained growth at elevated temperatures. Functional assays demonstrated extracellular proteolytic, amylolytic, and cellulolytic activities, mainly associated with B. licheniformis strains, in addition to tolerance to the pesticides fluazinam and benomyl. Antagonistic tests showed that B. licheniformis J8 inhibited the phytopathogenic fungi Sclerotinia sclerotiorum and Sclerotium rolfsii, while qualitative mineral solubilization assays indicated the ability of selected isolates to mobilize phosphate and potassium. These findings highlight geothermal ecosystems as valuable reservoirs of thermotolerant bacteria with enzymatic versatility and environmental relevance, supporting further molecular and process-optimization studies. Full article

Antimicrobial resistance [AMR] is a silent yet intensifying global threat, with particularly severe consequences in tropical and subtropical ecosystems, where high ecological connectivity, extensive antimicrobial use, and inadequate sanitation create ideal conditions for the persistence and spread of antimicrobial resistance genes [ARGs]. Within the One Health framework, migratory birds warrant special attention because they traverse tropical AMR hotspots, linking contaminated aquatic, agricultural, and peri-urban environments along established flyways. Evidence from tropical and subtropical regions indicates that migratory birds frequently carry clinically relevant AMR-associated pathogens, including extended-spectrum β-lactamase-producing Escherichia coli, multidrug-resistant Salmonella enterica, and fluoroquinolone- and macrolide-resistant Campylobacter spp. These findings suggest that migratory birds primarily function as ecological sentinels and geographic redistributors of antimicrobial resistance, reflecting environmental contamination and ecological connectivity between human-dominated and natural ecosystems, while evidence for long-term reservoir status remains context-dependent. Addressing the complex interface among AMR, migratory birds, and ARGs requires integrative surveillance strategies that explicitly incorporate wildlife into existing health systems. Genomic and metagenomic monitoring of migratory bird populations, combined with cross-sectoral data sharing, can provide early warning signals of emerging resistance patterns and inform evidence-based interventions. Understanding the ecological role of migratory birds in tropical ecosystems is therefore essential for designing effective One Health strategies to mitigate transboundary AMR risks and preserve the long-term efficacy of antimicrobial therapies. Full article

In today’s environment, rapid, reliable, and accurate bacterial detection is essential for protecting public health while preserving and ensuring the safety of food, water, and agricultural and environmental systems. Over the years, electrochemical sensors have gained widespread attention as viable candidates due to their rapid response, high sensitivity and selectivity, adaptability and portability, and low manufacturing cost. This facilitates their integration into various sectors, including healthcare and diagnostic applications, food safety and agriculture, and water and environmental monitoring. While these achievements represent tremendous progress, some of the challenges that need to be overcome include stability, batch-to-batch reproducibility, manufacturability, performance reliability, and the lack of point-of-care (POC) implementation for the utilization of these sensors for real-sample bacterial analysis. However, in the future, it is expected that with continued efforts made towards improving durability, standardization, and manufacturability, electrochemical bacterial sensors will be pivotal to the advancement of efficient bacterial diagnostics across various fields. This review presents major developments in modern electrochemical sensing technologies, which include, but are not limited to: electrochemical sensor and biosensor surface modifications, nanomaterials, the integration of artificial intelligence (AI) and machine learning (ML), and the emergence of wearable systems, for bacterial detection and monitoring. Additionally, their utilization in the aforementioned sectors is discussed. The integration and sustained use of these advanced electrochemical sensors for bacterial detection and surveillance can significantly enhance global safety and public well-being. Full article

Corynebacterium lactis was isolated from the skin abscess of a companion dog and from raw milk of a cow with unspecific mastitis. As information about the species was scarce and a zoonotic potential could not be excluded, we started a basic characterization of C. lactis strain RW3-42 with respect to antibiotic susceptibility and the response of invertebrate animal model systems to infection. C. lactis showed a number of antimicrobial resistances and is able to colonize Caenorhabditis elegans. In contrast, Galleria mellonella larvae were not impaired by C. lactis. Genome analyses of strain RW2-5 revealed the absence of toxin-encoding genes, and only a rather small number of other virulence factors were found, i.e., SpaA- and SpaH-type pili and the non-fimbrial adhesins DIP0733, DIP1281, DIP1621 and EmbC in addition to a homologue of Salmonella RhuM involved in killing of C. elegans. The results obtained indicate a limited pathogenic potential of the species. Full article

Prodigiosin, a red pigment with diverse biotechnological applications, is produced as a secondary metabolite by Gram-negative bacilli Serratia marcescens. In this study, we implemented an AI-guided hybrid optimization framework combining Response Surface Methodology (RSM) using a Circumscribed Central Composite Design (CCCD) and Artificial Neural Network (ANN) modeling to enhance prodigiosin pigment production. Across 34 experimental runs, we optimized sucrose and peptone concentrations along with inoculum size. The RSM-derived model exhibited a strong correlation (R² = 0.953), while the ANN, trained using a backpropagation algorithm, demonstrated superior predictive power (R² = 0.998; MSE = 0.000414), underscoring the potential of artificial intelligence in modeling complex bioprocesses. Beyond statistical optimization, an induction strategy using 1% of various natural additives (vegetable oils and egg components) identified egg white, rich in albumin, as the most effective enhancer, tripling prodigiosin yield. Further investigation revealed that a 2% egg white concentration maximized production to 1070 mg L⁻¹, a substantial increase compared to the optimized yield of 359.2 ± 12 mg L⁻¹ and predicted value of 391.86 mg L⁻¹. These results highlight the value of integrating machine learning with experimental design and protein-rich inducers to strengthen sustainable microbial pigment production in a cost-effective and scalable manner. Full article

Modern agriculture is increasingly challenged by fungal diseases, with phytopathogens such as Fusarium species causing substantial yield and quality losses in major crops globally. Although synthetic fungicides remain widely used, their intensive application raises serious concerns regarding environmental safety, human health, and the rapid emergence of resistant pathogen populations in the environment. These limitations have accelerated the search for sustainable, biologically based alternatives. In this context, Bacillus species isolated from saline and hypersaline habitats have emerged as a distinctive and still underexplored group of microorganisms with dual functionality as biological control agents (BCAs) and plant growth–promoting rhizobacteria (PGPRs) in salt-affected agroecosystems. Their novelty lies in their combined ability to suppress phytopathogens, enhance plant growth, and tolerate or mitigate salinity stress. Owing to their exceptional metabolic adaptability, these bacteria remain active under osmotic stress and produce a wide range of bioactive compounds that collectively contribute to their antifungal activity and improved plant performance. This review critically synthesizes advances published over the last six years (2019–2025), providing a comprehensive overview of the current understanding of the mechanisms underlying the biocontrol potential of halophilic/halotolerant Bacillus species against Fusarium spp. and other fungal phytopathogens. Particular emphasis is placed on ecological adaptations, molecular mechanisms, and the dual roles of these bacteria as BCAs and PGPR. The exploration and exploitation of saline-adapted Bacillus strains offer promising, eco-friendly, and cost-effective strategies for managing Fusarium diseases, thereby contributing to resilient and sustainable agricultural systems under increasing environmental constraints in the future. Full article

The increasing reliance on chemical fertilizers has raised environmental concerns and highlighted the need for sustainable alternatives. This study aimed to (i) optimize the carrier-to-substrate ratios and moisture content during composting with potassium-solubilizing purple nonsulfur bacteria (K-PNSB) and (ii) evaluate the growth-promoting effect of the optimized biofertilizer on maize seedlings. Three K-PNSB strains (Cereibacter sphaeroides M-Sl-09, Rhodopseudomonas thermotolerans M-So-11, and Rhodopseudomonas palustris M-So-14) were used. Composting experiments were conducted using different carrier-to-substrate ratios and moisture levels with K-PNSB inoculation. Compost quality was assessed through nutrient dynamics, bacterial density, and physicochemical properties over four weeks. The results showed that the 1:1:3 substrate ratio combined with 50–60% moisture content consistently enhanced K solubilization, bacterial survival, and compost maturity indicators. Application of the optimized biofertilizer improved maize growth traits compared with the non-inoculated control. These findings demonstrate that controlling material ratios and moisture content improves compost quality and plant growth performance, providing a sustainable alternative to chemical fertilizers. This study provides a practical framework for developing sustainable K-solubilizing biofertilizers from agricultural residues. Full article

Antimicrobial resistance (AMR) poses a severe threat to global health by limiting treatment options and increasing clinical and economic burdens. This review synthesizes evidence showing that resistance evolution is strongly shaped by antibiotic pressure, leading to the accumulation of adaptive mutations, activation of efflux systems, and widespread dissemination of resistance determinants across clinical, animal, and environmental settings. We highlight recent genomic, metagenomic, and structural findings that elucidate the molecular basis of AMR, with particular emphasis on horizontal gene transfer mediated by mobile genetic elements such as plasmids, integrons, and transposons. Analyses across One Health interfaces reveal extensive sharing of antimicrobial resistance genes among humans, livestock, and environmental reservoirs, identifying Enterobacteriaceae and ESKAPE pathogens as key hubs of resistance dissemination. Special focus is placed on Acinetobacter baumannii, where phylogenetic and three-dimensional structural analyses of class D β-lactamases OXA-23 and OXA-24/40 demonstrate a conserved catalytic framework coupled with substantial sequence and conformational variability. These structural differences likely influence carbapenem specificity and resistance levels. Collectively, the findings underscore how genetic diversity, mobile elements, and structural adaptation converge to drive AMR, reinforcing the need for integrated genomic and structural approaches to guide surveillance and antimicrobial development. Full article

The increasing prevalence of antimicrobial resistance, especially in methicillin-resistant Staphylococcus aureus (MRSA), underscores the need for alternative therapies from natural sources. This study investigated the chemical composition, antibacterial activity, and gene expression modulation of Melaleuca cajuputi essential oils. Gas chromatography–mass spectrometry (GC-MS) identified 91 compounds, with naphthalene (23.90%), guaiol (12.92%), caryophyllene oxide (9.69%), D-limonene 98% (8.59%), and gamma terpinene (7.54%) among the most abundant. In Silico molecular docking against MRSA virulence proteins revealed that alloaromadendrene had the strongest binding to toxic shock syndrome toxin-1 (TSST-1) (−7.948 kcal/mol), suggesting high inhibitory potential, while cyclohexane showed weak binding with staphylococcal enterotoxin A (SEA) (−3.532 kcal/mol). Antibacterial assays demonstrated concentration-dependent inhibition, with the zones ranging from 6.33 ± 0.33 mm to 16.67 ± 0.88 mm. MIC and MBC values ranged from 1.56 to 12.5% and 3.13 to 25%, respectively, with most isolates showing bactericidal effects (MBC/MIC ≤ 2). Gene expression analysis of MRSA isolate 4 indicated that sea was moderately upregulated (FC = 1.44), while sec remained unchanged (FC = 1.02). In contrast, fnbA (FC = 0.72), seb (FC = 0.33), and mecA (FC = 0.23) genes were downregulated, and the tsst-1 gene (FC = 0.05) was nearly silent. These findings highlight M. cajuputi essential oils as a promising candidate with both antibacterial efficacy and regulatory effects on MRSA virulence genes. Full article

Hot springs represent unique geothermal ecosystems where extreme physicochemical conditions intersect with remarkable microbial diversity and metabolic innovation. These natural laboratories harbor specialized communities of thermophilic and hyperthermophilic microorganisms that have evolved exceptional adaptations to elevated temperatures, extreme pH, and high salinity. This review synthesizes current understanding of hot spring systems as multifunctional natural resources, examining their roles in fundamental microbiology, biotechnology, and sustainable development. We explore the ecological principles governing microbial community assembly, the taxonomic and functional diversity of prokaryotic and eukaryotic microorganisms, and the genomic mechanisms underlying thermophilic adaptation. Hot springs yield enzymes revolutionizing molecular biology and industrial catalysis, bioactive metabolites with pharmaceutical potential, and novel bioremediation capabilities including plastic degradation. Beyond biological significance, these systems contain valuable minerals and rare earth elements, supporting an emerging bioeconomy integrating wellness tourism, bioprospecting, and sustainable resource extraction. However, critical knowledge gaps remain regarding viral ecology, horizontal gene transfer, eukaryotic diversity, and climate change impacts. We emphasize that hot springs merit renewed interdisciplinary attention as model systems for understanding extremophile physiology, early life evolution, and the development of nature-based biotechnological solutions. Realizing their full potential requires balanced management strategies that preserve ecosystem integrity while enabling responsible utilization of these irreplaceable geobiological resources. Full article

Sequencing technologies have reshaped the study of the subgingival microbiome, but selecting the appropriate method remains challenging because of differences in resolution, cost, host DNA contamination, and computational complexity. This review compares 16S rRNA sequencing, full-length 16S, shotgun metagenomics, and metatranscriptomics with respect to taxonomic resolution, functional output, sample requirements, and analytical limitations. Key practical issues, including low microbial biomass, contamination control, and the choice of appropriate bioinformatic tools, are emphasized to help researchers avoid common pitfalls. A decision-making framework is provided to link study goals to suitable sequencing methods while outlining realistic budget and sample-handling constraints. The review concludes with recommendations for integrating sequencing with complementary techniques to improve the accuracy, reproducibility, and clinical relevance of periodontal microbiome studies. Full article

(1) Background: Carbapenem-resistant Escherichia coli (CREC) is widespread and resistant to almost all available antimicrobial agents. In this study, we aimed to assess the phenotypic and molecular characteristics of CREC isolated from retail meats in Hat Yai, Songkhla, Thailand. (2) Methods: A total of 155 retail meat samples were randomly collected, and 412 presumptive carbapenem-non-susceptible isolates were screened via culturing on imipenem-containing eosin methylene blue (EMB) agar. Susceptibility to imipenem and meropenem was tested using the disk diffusion method, and carbapenemase and virulence genes in CREC isolates were detected using PCR. Phylogenetic groups and genetic relatedness of carbapenemase-positive CREC isolates were analyzed using gene markers and BOX-PCR, respectively. (3) Results: The results revealed a high prevalence of presumptive carbapenem-non-susceptible E. coli (CNSEC) isolates in beef samples. Over 89% of the CNSEC isolates from all meat types were identified as CREC. Of these, only 4.8% of the isolates from beef samples were positive for the bla_NDM gene, and one was also positive for the bla_VIM gene. These isolates carried only the fimH gene as a virulence factor. The bla_NDM-positive CREC isolates were classified in phylogenetic Group D. (4) Conclusions: Identifying antimicrobial-resistant pathogens, particularly CREC, in food-producing animals is critical due to potential risks to public health. Full article

Klebsiella pneumoniae is a prominent pathogen implicated in a wide range of infections, including pneumonia, urinary tract infections, and septicemia. Its ability to acquire and disseminate antibiotic resistance, coupled with the rising prevalence of hypervirulent strains, represents a significant public health threat. Understanding the molecular basis of drug resistance can guide the design and development of effective treatment strategies. Antimicrobial resistance (AMR) in these bacteria is a complicated process and cannot be attributed to a single resistance mechanism. K. pneumoniae develops resistance to antibiotics through a variety of mechanisms, ranging from single molecular mechanisms to complex interactions, where molecular synergy exacerbates resistance. This review summarizes the current understanding of the molecular mechanisms that contribute to the drug resistance and virulence of this pathogen. Key antibiotic resistance mechanisms include drug inactivation via B-lactamases and carbapenemases, membrane remodeling, efflux pump systems, such as AcrAB-TolC and OqxAB, and biofilm formation facilitated by quorum sensing. Additionally, the role of ribosomal changes in resistance is highlighted. This review also examines the mechanisms of virulence, emphasizing fimbriae, iron acquisition systems, and immune evasion strategies. Understanding these mechanisms of drug resistance and virulence is crucial for remodeling existing antibiotics and developing new therapeutic strategies. Full article

Background: Multidrug-resistant (MDR) Gram-negative bacilli (GNBs) significantly compromise the effective management of urinary tract infections (UTIs) worldwide. As antimicrobial resistance varies across regions, locally tailored data are essential to guide empirical therapy. This study investigated the prevalence, determinants, and temporal dynamics of MDR GNBs in UTI patients from Central Portugal between 2018 and 2022. Methods: We conducted a retrospective observational study at a hospital center in Central Portugal, analyzing data from 2018 to 2022. Data from 5194 UTI patients with GNB-positive cultures were analyzed. Binary logistic regression was used to identify determinants of MDR GNBs, defined as resistance to ≥1 agent in ≥3 antibiotic classes. Results: The study population had a mean age of 64.5 ± 25.3 years, and females represented two-thirds of the sample (67.0%). The overall prevalence of MDR GNBs was 35.8%. Advanced age (≥75 years), male sex, and specific treatment contexts—particularly day treatment and laboratory-only cases—were independently associated with MDR. SBL-producing Enterobacterales and non-fermenting GNBs showed the highest risk levels. Conclusions: MDR GNBs are highly prevalent among UTI patients in Central Portugal, and their increasing trend—particularly in 2022—highlights an urgent need for strengthened surveillance and updated empirical treatment strategies. The observed temporal increase highlights the urgent need for strengthened regional surveillance and updated empirical treatment guidelines. Full article

The taxonomy of the genus Arcobacter has been subject to substantive turmoil in recent years following a proposal to subdivide the genus into six genera. This proposal has been challenged by a number of multidisciplinary studies employing phenotypic, genomic, and phylogenetic analyses. Following several discussions among members of the International Committee on Systematics of Prokaryotes (ICSP) subcommittee on the taxonomy of Campylobacter and related bacteria, this group now unanimously recommends the use of the genus term Arcobacter to refer to these species. Full article

Streptococcus uberis is a bovine mastitis pathogen with a demonstrated ability to form biofilms. However, the dynamics of this process remain poorly characterized. This study aimed to comprehensively characterize biofilm formation in four S. uberis strains that differed in their biofilm-forming capacity, from weak to strong producers, and in the presence of key virulence-associated genes, such as sua, hasA and hasC. To achieve this, we integrated structural, biochemical, physiological and transcriptional analyses using scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), spectral flow cytometry and qRT-PCR. The multi-faceted analysis revealed a coordinated maturation peak at 48 h, characterized by a structured architecture with water channels, a distinct biochemical signature rich in polysaccharides and proteins, and a predominantly viable bacterial population. This peak coincided with a marked upregulation of key virulence-associated genes, with sua expression increasing 2.5-fold and hasA increasing 3-fold at 48 h. This mature biofilm conferred high tolerance to antibiotics, with eradication concentrations (>256 µg/mL) exceeding planktonic MICs, although tetracycline was notably effective. At 72 h, the biofilm entered a dispersion phase characterized by structural collapse and reduced viability. These findings establish S. uberis biofilm maturation as a highly coordinated process, providing new insights into the biofilm lifecycle of this important pathogen and identifying key temporal and molecular targets for future interventions. Full article

Globally, large quantities of animal waste and human sewage sludge are generated annually. Their application as soil amendments can enhance soil quality and support a circular economy. However, these wastes may harbour pathogenic bacteria, posing contamination risks to soil and water and potential transmission to animals and humans. This study investigated the survival of five bacterial pathogens during six months of storage in five types of organic waste and following their subsequent application to soil. During storage, T₉₀ values ranged as follows: Salmonella Typhimurium (2.3–17.7 days), Campylobacter jejuni (0 to 23.9 days), Escherichia coli O157:H7 (4.3 to 57.8 days), and Listeria monocytogenes (1.9 to 170.4 days). In soil, T₉₀ values were S. Typhimurium (4.2 to 17.4 days), C. jejuni (4.8 to 26.8 days), E. coli O157:H7 (4.3 to 52.9 days), and L. monocytogenes (2 to 83.7 days). Clostridium sporogenes remained stable throughout both experiments, preventing T₉₀ calculation. Contrary to our initial hypothesis that soil microbiota would accelerate pathogen decline, T₉₀ values were higher during storage in 11 cases and higher in soil in nine scenarios. These findings highlight the need for pre-treatment strategies for animal waste and biosolids before land spreading to consistently mitigate risks of pathogen transmission and environmental contamination. Full article

Perinatal depression (PND) is a severe mood disorder affecting mothers during pregnancy and postpartum, with implications for both maternal and neonatal health. Emerging evidence suggests that gut microbiota-derived metabolites play a critical role in neuroinflammation and neurotransmission. In this study, we employed an in silico approach to evaluate the pharmacokinetic and therapeutic potential of metabolites produced by Lactobacillus helveticus and Bifidobacterium longum in targeting key proteins implicated in PND, including BDNF, CCL2, TNF, IL17A, IL1B, CXCL8, IL6, IL10. The ADMET (Absorption, Distribution, Metabolism, Excretion, and Toxicity) profiles of selected microbial metabolites, including acetate, lactate, formate, folic acid, riboflavin, kynurenic acid, γ-aminobutyric acid, and vitamin B12 were assessed using computational tools to predict their bioavailability and safety. Enrichment analysis was performed to identify biological pathways and molecular mechanisms modulated by these metabolites, with a focus on neuroinflammation, stress response, and neurogenesis. Additionally, molecular docking studies were conducted to evaluate the binding affinities of these metabolites toward the selected PND-associated targets, providing insights into their potential as neuroactive agents. Our findings suggest that specific microbial metabolites exhibit favorable ADMET properties and strong binding interactions with key proteins implicated in PND pathophysiology. These results highlight the therapeutic potential of gut microbiota-derived metabolites in modulating neuroinflammatory and neuroendocrine pathways, paving the way for novel microbiome-based interventions for perinatal depression. Further experimental validation is warranted to confirm these computational predictions and explore the clinical relevance of these findings. Full article

This study focuses on discovering novel quorum-sensing inhibitors (QSIs) from endophytes of Coelothrix irregularis, aiming to develop new strategies against drug-resistant bacterial infections. From the endophytic bacterial strain Bacillus strain W10-B1, isolated from C. irregularis, twelve compounds were isolated and structurally identified. Subsequent screening against Serratia marcescens NJ01 revealed that compound (12), 3,3′-dibromo-4,4′-biphenyldiol, exhibited significant inhibitory activity against the quorum-sensing system of S. marcescens NJ01. It effectively suppressed biofilm formation, swimming, and swarming motilities of the bacterium. This work is the first to demonstrate that endophytes from C. irregularis are a novel source of potent QSIs, providing both material and theoretical foundations for combating pathogen virulence, drug resistance, and pathogenicity. Full article