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Bioengineering, Volume 12, Issue 6

June 2025 - 119 articles

Cover Story: A novel protein engineering approach enhances the crystallization of the leaf branch compost cutinase (LCC) quadruple mutant ICCG, a key enzyme for effective PET recycling under mild conditions. By introducing targeted electrostatic interactions—specifically Arg–Glu pairs—at crystal contact sites, researchers generated the ICCGY T110E mutant with improved crystallizability in terms of faster crystallization at decreased protein concentrations. Controlled mutations at non-interacting sites confirmed the specificity of this strategy, with preservation of activity. This advancement facilitates more efficient downstream processing of biocatalysts, offering a rational method to streamline enzyme purification and support sustainable industrial biotechnology applications. View this paper
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Articles (119)

  • Article
  • Open Access
2 Citations
1,306 Views
35 Pages

ResST-SEUNet++: Deep Model for Accurate Segmentation of Left Ventricle and Myocardium in Magnetic Resonance Imaging (MRI) Images

  • Abduljabbar S. Ba Mahel,
  • Mehdhar S. A. M. Al-Gaashani,
  • Fahad Mushabbab G. Alotaibi and
  • Reem Ibrahim Alkanhel

The highly precise and trustworthy segmentation of the left ventricle (LV) and myocardium is critical for diagnosing and treating cardiovascular disorders, which includes persistent microvascular obstruction (MVO) as well as myocardial infarction (MI...

  • Article
  • Open Access
1 Citations
1,461 Views
18 Pages

Effects of Physiological Loading from Patient-Derived Activities of Daily Living on the Wear of Metal-on-Polymer Total Hip Replacements

  • Benjamin A. Clegg,
  • Samuel Perry,
  • Enrico De Pieri,
  • Anthony C. Redmond,
  • Stephen J. Ferguson,
  • David E. Lunn,
  • Richard M. Hall,
  • Michael G. Bryant,
  • Nazanin Emami and
  • Andrew R. Beadling

The current pre-clinical testing standards for total hip replacements (THRs), ISO standards, use simplified loading waveforms that do not fully replicate real-world biomechanics. These standards provide a benchmark of data that may not accurately pre...

  • Article
  • Open Access
1 Citations
1,534 Views
17 Pages

The REAtouch® Lite device was recently developed to support motor skill learning-based interventions, integrating both games/activities and assessment tools to enable home-based telerehabilitation. Given the importance of hand functions in rehabi...

  • Brief Report
  • Open Access
1 Citations
1,529 Views
11 Pages

In this comprehensive study, we delve into the intricate binding properties of tannic acid (TA) and examine their dual role in the realm of biomaterial development. While TA’s properties can enhance the functionality and performance of biomater...

  • Article
  • Open Access
2 Citations
4,242 Views
13 Pages

The knee is one of the most frequently injured joints, involving various structures. To prevent reinjury after rehabilitation, braces are commonly used. However, most studies on knee supports focus on subjects with anterior cruciate ligament (ACL) in...

  • Article
  • Open Access
2,060 Views
18 Pages

In postmenopausal women, the prevalence of hypertension increases sharply, emphasizing the importance of its prevention. This increased risk highlights the critical need for effective prevention strategies specifically designed for this population. T...

  • Article
  • Open Access
2 Citations
1,110 Views
21 Pages

BioGAN: Enhancing Transcriptomic Data Generation with Biological Knowledge

  • Francesca Pia Panaccione,
  • Sofia Mongardi,
  • Marco Masseroli and
  • Pietro Pinoli

The advancement of computational genomics has significantly enhanced the use of data-driven solutions in disease prediction and precision medicine. Yet, challenges such as data scarcity, privacy constraints, and biases persist. Synthetic data generat...

  • Article
  • Open Access
3,718 Views
13 Pages

Cell-Based Therapies: Ferromagnetic Versus Superparamagnetic Cell Targeting

  • Tasneem Halhouli,
  • Lisa Münchhalfen,
  • Sarkawt Hamad,
  • Larissa Schmitz-Ullrich,
  • Frank Nitsche,
  • Felix Gaedke,
  • Astrid Schauss,
  • Linlin Zhang,
  • Quoc-Khanh Pham and
  • Gang Bao
  • + 1 author

Stem-cell-based therapies rely on the transplantation of stem cells or stem-cell-derived organotypic cells into injured tissues in order to improve or restore tissue function that has been impaired by various diseases. The potential of induced plurip...

  • Article
  • Open Access
1,363 Views
16 Pages

Anatomical Characteristics Predict Response to Transcranial Direct Current Stimulation (tDCS): Development of a Computational Pipeline for Optimizing tDCS Protocols

  • Giulia Caiani,
  • Emma Chiaramello,
  • Marta Parazzini,
  • Eleonora Arrigoni,
  • Leonor J. Romero Lauro,
  • Alberto Pisoni and
  • Serena Fiocchi

Transcranial direct current stimulation (tDCS) is a non-invasive brain stimulation technique promisingly used to treat neurological and psychological disorders. Nevertheless, the inter-subject heterogeneity in its after-effects frequently limits its...

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Bioengineering - ISSN 2306-5354