Long-chain imidazolium-based ionic liquids (ILs) possess a broad-spectrum biological activity and are considered promising antifouling agents for protective coatings. A new hydrophobic IL, 1-dodecyl-3-methylimidazolium dodecylbenzenesulfonate (C
12C
1IM-DBS), has been synthesized, and a modified epoxy coating material containing 10, 20, and
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Long-chain imidazolium-based ionic liquids (ILs) possess a broad-spectrum biological activity and are considered promising antifouling agents for protective coatings. A new hydrophobic IL, 1-dodecyl-3-methylimidazolium dodecylbenzenesulfonate (C
12C
1IM-DBS), has been synthesized, and a modified epoxy coating material containing 10, 20, and 30 wt% of this IL was prepared by dissolution of C
12C
1IM-DBS in commercial DER 331 epoxy resin, followed by a curing phase with diethylenetriamine. Infrared analysis revealed physicochemical interactions between the hydroxyl groups of the resin and the IL. Spectrophotometric studies showed no release of C
12C
1IM-DBS after 30 days of exposure of the modified coatings to water. The plasticizing effect of the IL on the epoxy resin was established by differential scanning calorimetry analysis. The introduction of 10 and 20% C
12C
1IM-DBS into DER 331 reduced its glass transition temperature from 122.8 °C to 109.3 and 91.5 °C, respectively. The hardness of epoxy resin decreased by approximately 26% after the introduction of the IL. Moreover, DER 331/C
12C
1IM-DBS coatings on steel substrates showed significantly improved impact resistance compared to neat resin. The antibiofilm efficiency of DER 331/C
12C
1IM-DBS coatings was evaluated by assessing the capability of two biofilm-forming model strains,
Staphylococcus aureus ATCC 25923 and
Pseudomonas aeruginosa PA01, to form attached biofilms on the surface. The IL effectively inhibited
S. aureus surface-associated biofilm development even at the lowest content of 10%. On the contrary, an approximately 50% inhibition of biofilm metabolic activity was detected for DER 331/C
12C
1IM-DBS coatings containing 20% and 30% of the IL. Overall, the results of this study indicate that the hydrophobic IL C
12C
1IM-DBS is an efficient modifying additive for epoxy resins, which can significantly improve their operational properties for various industrial applications.
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