Driven by climate change and human activities, the expansion of highly invasive moso bamboo (
Phyllostachys edulis) into coniferous forests induces a serious ecological imbalance. Its rapidly spreading underground roots significantly alter soil structure, yet the mechanisms by which this expansion affects soil detachment capacity (
Dc), a key soil erosion parameter, remain unclear. While bamboo expansion modifies soil physicochemical properties and root characteristics, influencing
Dc and, consequently, soil erosion resistance, the underlying mechanisms, particularly stage-specific variations, are not thoroughly understood. In this study, we examined Japanese white pine (
Pinus parviflora Siebold & Zucc.) forest (CF), moso bamboo–Japanese white pine mixed forest (MF), and moso bamboo forest (BF) as representative stages of bamboo expansion. By integrating laboratory-controlled measurements of soil physicochemical properties and root traits with field-based flume experiments, we comprehensively investigate the effects of moso bamboo expansion into CF on soil detachment capacity. The results of the study can be summarized as follows: (1) Expansion of moso bamboo significantly changed soil physicochemical properties and root characteristics. Soil bulk density was the highest in the MF (1.13 g·cm
−3), followed by the CF (1.08 g·cm
−3) and BF (1.03 g·cm
−3); non-capillary porosity increased significantly with expansion (CF 0.03% to MF 0.10%); and although the stability of aggregates (MWD) increased by 24.5% from the CF to MF, root mass density (RMD) in the MF (0.0048 g·cm
−3) was much higher than that in the CF (0.0009 g·cm
−3). This intense root competition between forest types, combined with increased macroporosity development, compromised overall soil structural integrity. This weakening may lead to a looser soil structure during the transition phase, thereby increasing erosion risk. (2) There were significant stage differences in
Dc: it was significantly higher in the MF (0.034 kg·m
−2·s
−1) than in the CF (0.023 kg·m
−2·s
−1) and BF (0.018 kg·m
−2·s
−1), which revealed that the MF was an erosion-sensitive stage. (3) Our Partial Least Squares Structural Equation Modeling (PLS-SEM) results revealed that soil physicochemical properties (soil moisture content and soil total nitrogen) dominated
Dc changes through direct effects (total effect −0.547); in comparison, root properties indirectly affected
Dc by modulating soil structure (indirect effect: −0.339). The results of this study reveal the dynamics and mechanisms of
Dc changes during bamboo expansion, and for the first time, we identify a distinct
Dc peak during the mixed forest transition phase. These findings provide a scientific basis for moso bamboo forest management, soil erosion risk assessment, and optimization of soil and water conservation strategies.
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