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Microorganisms, Volume 8, Issue 5

May 2020 - 171 articles

Cover Story: Filamentous bacteriophages are promising immunogenic carriers for antigen delivery. Their use is based on the modification of phage display technology; in particular, multiple copies of exogenous peptides (or polypeptides) can be covalently linked to viral capsid proteins and expressed on the phage coat. This study, for the first time, proposes the use of polymeric poly (lactic-co-glycolic acid) (PLGA) microparticles (MPs), to encapsulate fd bacteriophage displaying antigenic peptides and to demonstrate the immunogenicity of the encapsulated bacteriophages after their release by MPs. Our results show that PLGA MPs-encapsulated bacteriophages are stable and retain their immunogenic properties, demonstrating that microparticles can represent an important tool for the development of bacteriophage-based immunotherapeutic platforms. View this paper
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Articles (171)

  • Review
  • Open Access
20 Citations
7,159 Views
13 Pages

Influenza viruses arise from animal reservoirs, and have the potential to cause pandemics. In 2013, low pathogenic novel avian influenza A(H7N9) viruses emerged in China, resulting from the reassortment of avian-origin viruses. Following evolutionary...

  • Editorial
  • Open Access
2 Citations
2,387 Views
2 Pages

In recent years, increased resistance to antibiotics and disinfectants from foodborne bacterial pathogens has become a relevant consumer health issue and a growing concern for food safety authorities [...]

  • Article
  • Open Access
22 Citations
10,654 Views
18 Pages

Evaluation of a Highly Efficient DNA Extraction Method for Bacillus anthracis Endospores

  • Mandy Knüpfer,
  • Peter Braun,
  • Kathrin Baumann,
  • Alexandra Rehn,
  • Markus Antwerpen,
  • Gregor Grass and
  • and Roman Wölfel

A variety of methods have been established in order to optimize the accessibility of DNA originating from Bacillus anthracis cells and endospores to facilitate highly sensitive molecular diagnostics. However, most endospore lysis techniques have not...

  • Article
  • Open Access
17 Citations
3,606 Views
16 Pages

Entrapped Psychrotolerant Yeast Cells within Pine Sawdust for Low Temperature Wine Making: Impact on Wine Quality

  • Antonia Terpou,
  • Vassilios Ganatsios,
  • Maria Kanellaki and
  • Athanasios A. Koutinas

An alternative methodology is proposed for low temperature winemaking using freeze-dried raw materials. Pine sawdust was delignified and the received porous cellulosic material was applied as immobilization carrier of the psychrotolerant yeast strain...

  • Perspective
  • Open Access
16 Citations
5,538 Views
15 Pages

Proteomics of Brucella: Technologies and Their Applications for Basic Research and Medical Microbiology

  • Gamal Wareth,
  • Mathias W. Pletz,
  • Heinrich Neubauer and
  • Jayaseelan Murugaiyan

Brucellosis is a global zoonosis caused by Gram-negative, facultative intracellular bacteria of the genus Brucella (B.). Proteomics has been used to investigate a few B. melitensis and B. abortus strains, but data for other species and biovars are li...

  • Article
  • Open Access
25 Citations
5,070 Views
18 Pages

Dual-Purpose Inoculants and Their Effects on Corn Silage

  • Dimas Hand Vidya Paradhipta,
  • Seong Shin Lee,
  • Byeongsam Kang,
  • Young Ho Joo,
  • Hyuk Jun Lee,
  • Yeyeong Lee,
  • Jinwoo Kim and
  • Sam Churl Kim

This study was conducted to screen dual-purpose lactic acid bacteria (LAB) from uncontrolled farm-scale silage, and then we confirmed their effects on corn silage. The LAB were isolated from eight farm-scale corn silages, and then we screened the ant...

  • Article
  • Open Access
24 Citations
3,978 Views
11 Pages

Environmental Impact on Differential Composition of Gut Microbiota in Indoor Chickens in Commercial Production and Outdoor, Backyard Chickens

  • Zuzana Seidlerova,
  • Tereza Kubasova,
  • Marcela Faldynova,
  • Magdalena Crhanova,
  • Daniela Karasova,
  • Vladimir Babak and
  • Ivan Rychlik

In this study, we compared the caecal microbiota composition of egg-laying hens from commercial production that are kept indoors throughout their whole life with microbiota of hens kept outdoors. The microbiota of outdoor hens consisted of lower numb...

  • Article
  • Open Access
19 Citations
5,526 Views
18 Pages

The Methylocystis and Methylosinus are two of the five genera that were included in the first taxonomic framework of methanotrophic bacteria created half a century ago. Members of both genera are widely distributed in various environments and play a...

  • Article
  • Open Access
5 Citations
4,407 Views
16 Pages

Proteome Cold-Shock Response in the Extremely Acidophilic Archaeon, Cuniculiplasma divulgatum

  • Rafael Bargiela,
  • Karin Lanthaler,
  • Colin M. Potter,
  • Manuel Ferrer,
  • Alexander F. Yakunin,
  • Bela Paizs,
  • Peter N. Golyshin and
  • Olga V. Golyshina

The archaeon Cuniculiplasma divulgatum is ubiquitous in acidic environments with low-to-moderate temperatures. However, molecular mechanisms underlying its ability to thrive at lower temperatures remain unexplored. Using mass spectrometry (MS)-based...

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Microorganisms - ISSN 2076-2607