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Life, Volume 10, Issue 5

May 2020 - 26 articles

Cover Story: Human articular chondrocytes (HACs) synthetize the extracellular matrix (ECM) that confer cartilage its biomechanical properties. HACs failure to produce a balanced ECM leads to osteoarthritis and inflammation worsen the disease. We have shown that miRNA miR-145 silences transcription factor Sox9 resulting in lower and higher levels, respectively, of the main ECM component Col2a1 and the catabolic protease MMP13. Here, by immunoprecipitating the miRNA-induced silencing complex (RISC) in HACs overexpressing miR-145, we identify more than 50 miR-145 new direct targets. One of these targets, DUSP6, contributes to MMP13 repression. Overexpression of miR-145 results in altered expression of hundreds of genes involved in ECM remodelling and immunity and inflammation. Thus, we propose miR-145 inhibition as a potential approach for cartilage repair. View this paper
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Articles (26)

  • Review
  • Open Access
37 Citations
6,648 Views
21 Pages

2 May 2020

Polyphenols are plant secondary metabolites with diverse biological and potential therapeutic activities such as antioxidant, anti-inflammatory and anticancer, among others. However, their extraction from the native plants is not enough to satisfy th...

  • Article
  • Open Access
7 Citations
3,767 Views
16 Pages

30 April 2020

DnaK3, a highly conserved cyanobacterial chaperone of the Hsp70 family, binds to cyanobacterial thylakoid membranes, and an involvement of DnaK3 in the biogenesis of thylakoid membranes has been suggested. As shown here, light triggers synthesis of D...

  • Article
  • Open Access
4 Citations
3,856 Views
16 Pages

The Nitrogen Stress-Repressed sRNA NsrR1 Regulates Expression of all1871, a Gene Required for Diazotrophic Growth in Nostoc sp. PCC 7120

  • Isidro Álvarez-Escribano,
  • Manuel Brenes-Álvarez,
  • Elvira Olmedo-Verd,
  • Agustín Vioque and
  • Alicia M. Muro-Pastor

29 April 2020

Small regulatory RNAs (sRNAs) are post-transcriptional regulators of bacterial gene expression. In cyanobacteria, the responses to nitrogen availability, that are mostly controlled at the transcriptional level by NtcA, involve also at least two small...

  • Communication
  • Open Access
40 Citations
11,692 Views
8 Pages

28 April 2020

The habitability of Mars is strongly dependent on the availability of liquid water, which is essential for life as we know it. One of the few places where liquid water might be found on Mars is in liquid perchlorate brines that could form via delique...

  • Article
  • Open Access
4 Citations
3,614 Views
14 Pages

27 April 2020

The expression of neuropeptide Y (NPY) in the arcuate nucleus (ARC) and corticotropin-releasing factor (CRF) in the paraventricular nucleus (PVN) were increased when low body weight–selected (LWS) line chicks, which are predisposed to anorexia,...

  • Review
  • Open Access
27 Citations
19,138 Views
34 Pages

27 April 2020

Two widely-cited alternative hypotheses propose geological localities and biochemical mechanisms for life’s origins. The first states that chemical energy available in submarine hydrothermal vents supported the formation of organic compounds an...

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Life - ISSN 2075-1729