Sign in to use this feature.

Years

Between: -

Subjects

remove_circle_outline

Journals

Article Types

Countries / Regions

Search Results (1)

Search Parameters:
Keywords = DO-based fed-batch

Order results
Result details
Results per page
Select all
Export citation of selected articles as:
31 pages, 4210 KB  
Article
Optimized Feeding Strategies for Biosurfactant Production from Acetate by Alcanivorax borkumensis SK2
by Tobias Karmainski, Marie K. Lipa, Sonja Kubicki, Amina Bouchenafa, Stephan Thies, Karl-Erich Jaeger, Lars M. Blank and Till Tiso
Fermentation 2024, 10(5), 257; https://doi.org/10.3390/fermentation10050257 - 14 May 2024
Cited by 9 | Viewed by 4116
Abstract
Biosurfactants are much-discussed alternatives to petro- and oleochemical surfactants. Alcanivorax borkumensis, a marine, Gram-negative γ-proteobacterium, produces a glycine-glucolipid biosurfactant from hydrocarbons, pyruvate, and acetate as carbon sources. Sustainable acetate production from lignocellulose or syngas adds to its relevance for the bioeconomy. This [...] Read more.
Biosurfactants are much-discussed alternatives to petro- and oleochemical surfactants. Alcanivorax borkumensis, a marine, Gram-negative γ-proteobacterium, produces a glycine-glucolipid biosurfactant from hydrocarbons, pyruvate, and acetate as carbon sources. Sustainable acetate production from lignocellulose or syngas adds to its relevance for the bioeconomy. This study investigated nitrogen sources and carbon-to-nitrogen ratios (C/N) to optimize fed-batch fermentation for biosurfactant production using A. borkumensis with acetate as the carbon source. Urea enabled high biosurfactant production, which was confirmed in DO-based fed-batch fermentation. Varying C/N ratios led to increased glycine-glucolipid production and decreased biomass production, with improvement plateauing at a C/N ratio of 26.7 Cmol Nmol−1. pH-stat fed-batch fermentation using glacial acetic acid as the carbon source and a pH-adjusting agent doubled the biosurfactant production. Finally, bubble-free membrane aeration was used to prevent extensive foam formation observed during conventional bubble aeration. The efficient production made it possible to investigate the bioactivity of glycine-glucolipid in combination with antibiotics against various microorganisms. Our findings allow for the leverage of glycine-glucolipid biosurfactant production using acetate as a carbon source. Full article
(This article belongs to the Special Issue Production and Application of Bioactive Biosurfactants)
Show Figures

Figure 1

Back to TopTop