Advanced Analytical Technologies and Biosensors for Food Safety and Antimicrobial Resistance Detection

A special issue of Foods (ISSN 2304-8158). This special issue belongs to the section "Food Analytical Methods".

Deadline for manuscript submissions: 30 September 2025 | Viewed by 806

Special Issue Editor


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Guest Editor
REQUIMTE/LAQV, Department of Chemical Engineering, Faculty of Engineering, University of Porto, 4200-465 Porto, Portugal
Interests: analytical chemistry; eectroanalysis; biosensors (DNA-based, enzymatic, immunosensors, bacteriophage-based); nanomaterials; isothermal DNA amplification; rapid foodborne pathogen detectio; antimicrobial resistance; food safety
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Special Issue Information

Dear Colleagues,

Antimicrobial resistance (AMR) in foodborne pathogens poses a serious public health threat, complicating efforts to ensure food safety and requiring advanced technologies for effective risk assessments and its mitigation. This Special Issue focuses on the latest developments in analytical technologies and biosensors designed to improve the detection of AMR in food systems.

Its key topics include cutting-edge sensor platforms, such as electrochemical and optical sensors, microfluidics, lab-on-a-chip systems, and portable diagnostics, which can all be tailored for the rapid on-site detection of AMR pathogens and resistance genes. Molecular diagnostics like PCR, isothermal DNA amplification, and CRISPR-based assays are highlighted for their precision in identifying specific AMR genes within complex food matrices. These techniques, when combined with biosensing platforms, enhance the sensitivity and specificity of bacterial detection, providing early warnings to prevent the spread of resistant strains.

Innovative nanomaterials, including graphene and metal–organic frameworks (MOFs), are being explored for their ability to lower detection limits and enable the simultaneous detection of multiple pathogens. Smart packaging and portable sensors capable of real-time AMR monitoring are discussed as essential tools for managing risks throughout the food supply chain. These advancements in molecular biology and sensor technologies are critical for the rapid detection of AMR and effective mitigation strategies, ultimately supporting enhanced food safety practices and public health protection.

Dr. Júlia Magalhães
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Foods is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2900 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • antimicrobial resistance (AMR)
  • foodborne pathogens
  • biosensors
  • molecular diagnostics
  • electrochemical sensors
  • microfluidics
  • CRISPR-based assays
  • nanomaterials
  • real-time monitoring
  • food safety

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Published Papers (1 paper)

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Research

12 pages, 3925 KiB  
Article
Ratiometric Fluorescent Probes Based on Isosteviol with Identification of Maleic Acid in Starchy Foods
by Xinye Qian, Chunling Zheng and Fang Zhang
Foods 2025, 14(9), 1541; https://doi.org/10.3390/foods14091541 - 28 Apr 2025
Viewed by 154
Abstract
The rigid saddle-shaped framework of isosteviol provides a unique host–guest recognition cavity. For the first time, we have utilized isosteviol to construct fluorescent probes 4 and 5, achieving highly selective recognition of maleic acid and fumaric acid. The experimental results indicated that neither [...] Read more.
The rigid saddle-shaped framework of isosteviol provides a unique host–guest recognition cavity. For the first time, we have utilized isosteviol to construct fluorescent probes 4 and 5, achieving highly selective recognition of maleic acid and fumaric acid. The experimental results indicated that neither probe 4 nor probe 5 exhibited significant fluorescence changes when exposed to fumaric acid. However, both probes demonstrated distinct ratiometric fluorescence responses upon interaction with maleic acid. For maleic acid, probes 4 and 5 showed detection limits of 4.14 × 10−6 M and 1.88 × 10−6 M, respectively. Density functional theory (DFT) calculations and 1H NMR spectroscopy revealed that probes 4 and 5 formed stable intermolecular hydrogen bonds with maleic acid, contributing to the observed changes in fluorescence signals. Furthermore, maleic acid was successfully detected in starch-rich dietary samples, including potatoes, sweet potatoes, and corn, utilizing the sensing capabilities of probes 4 and 5. In conclusion, probes 4 and 5 hold significant potential for the development of fluorescence-based recognition systems for fumaric acid and maleic acid. Full article
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