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Detection Strategies and Risk Assessment of Genetically Modified Foods
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Detection Strategies and Risk Assessment of Genetically Modified Foods

A special issue of Foods (ISSN 2304-8158). This special issue belongs to the section "Food Analytical Methods".

Deadline for manuscript submissions: closed (18 November 2024) | Viewed by 6484

Special Issue Editor

Dr. Xiaoyun He

E-Mail Website
Guest Editor
1. Key Laboratory for Safety Evaluation of Agricultural Genetically Modified Organisms, Ministry of Agriculture and Rural Affairs of the People’s Republic of China, Beijing 100083, China
2. Key Laboratory of Precision Nutrition and Food Quality, Key Laboratory of Functional Dairy, Ministry of Education, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
Interests: food safety; risk assessment; food nutrition; genetically modified food
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The development of genetically modified (GM) or bioengineering (BE) technology, especially GM foods, has brought extremely high economic and nutritional improvements as well as social benefits to human society. However, the risks of GM technology has always been controversial, with potential risks to the ecological environment and consumers due to its potential allergeniticy and toxicity. With the maturity and quick development of GM technology, such as gene editing technology, more and more novel GM foods are being developed. Therefore, it is particularly important to develop rapid and effective detection strategies and risk assessment methods for GM foods. Different analytical methods have been developed in recent years, including targeted analysis and state-of-the-art analytical strategies based on omics technology. Despite these advances, more robust, reliable and comprehensive methods to facilitate the rapid development of bioengineering technology to evaluate the safety and quality of GM/BE foods are still needed. This Special Issue welcomes contributions related to GM food safety assessment and detection strategies and discusses the development trends and sustainability of GM foods.

Dr. Xiaoyun He
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Foods is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2900 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • genetically modified/bioengineering foods and feeds
  • food/feed safety
  • gene editing
  • CRISPR
  • nutritional composition
  • allergenicity
  • toxicity
  • unintended effect
  • omics
  • detection strategies
  • rapid detection
  • sustainable development

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Published Papers (3 papers)

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14 pages, 509 KiB  
Article
The Development of a Series of Genomic DNA Reference Materials with Specific Copy Number Ratios for The Detection of Genetically Modified Maize DBN9936
by Jun Li, Hongfei Gao, Yunjing Li, Shanshan Zhai, Fang Xiao, Gang Wu and Yuhua Wu
Foods 2024, 13(5), 747; https://doi.org/10.3390/foods13050747 - 28 Feb 2024
Cited by 3 | Viewed by 1512
Abstract
The genetically modified (GM) maize DBN9936 with a biosafety certificate will soon undergo commercial application. To monitor the safety of DBN9936 maize, three genomic DNA (gDNA) reference materials (RMs) (DBN9936a, DBN9936b, and DBN9936c) were prepared with nominal copy number ratios of 100%, 3%, [...] Read more.
The genetically modified (GM) maize DBN9936 with a biosafety certificate will soon undergo commercial application. To monitor the safety of DBN9936 maize, three genomic DNA (gDNA) reference materials (RMs) (DBN9936a, DBN9936b, and DBN9936c) were prepared with nominal copy number ratios of 100%, 3%, and 1% for the DBN9936 event, respectively. DBN9936a was prepared from the leaf tissue gDNA of DBN9936 homozygotes, while DBN9936b and DBN9936c were prepared by the quantitative mixing of gDNA from the leaf tissues of DBN9936 homozygotes and non-GM counterparts. Validated DBN9936/zSSIIb duplex droplet digital PCR was demonstrated to be an accurate reference method for conducting homogeneity study, stability study, and collaborative characterization. The minimum intake for one measurement was determined to be 2 μL, and the gDNA RMs were stable during transport at 37 °C for 14 days and storage at −20 °C for 18 months. Each gDNA RM was certified for three property values: DBN9936 event copy number concentration, zSSIIb reference gene copy number concentration, and DBN9936/zSSIIb copy number ratio. The measurement uncertainty of the certified values took the uncertainty components related to possible inhomogeneity, instability, and characterization into account. This batch of gDNA RMs can be used for calibration and quality control when quantifying DBN9936 events. Full article
(This article belongs to the Special Issue Detection Strategies and Risk Assessment of Genetically Modified Foods)
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23 pages, 1429 KiB  
Article
Strategies for Traceability to Prevent Unauthorised GMOs (Including NGTs) in the EU: State of the Art and Possible Alternative Approaches
by Jenny Teufel, Viviana López Hernández, Anita Greiter, Nele Kampffmeyer, Inga Hilbert, Michael Eckerstorfer, Frank Narendja, Andreas Heissenberger and Samson Simon
Foods 2024, 13(3), 369; https://doi.org/10.3390/foods13030369 - 23 Jan 2024
Cited by 5 | Viewed by 3105
Abstract
The EU’s regulatory framework for genetically modified organisms (GMOs) was developed for “classical” transgenic GMOs, yet advancements in so-called “new genomic techniques (NGTs)” have led to implementation challenges regarding detection and identification. As traceability can complement detection and identification strategies, improvements to the [...] Read more.
The EU’s regulatory framework for genetically modified organisms (GMOs) was developed for “classical” transgenic GMOs, yet advancements in so-called “new genomic techniques (NGTs)” have led to implementation challenges regarding detection and identification. As traceability can complement detection and identification strategies, improvements to the existing traceability strategy for GMOs are investigated in this study. Our results are based on a comprehensive analysis of existing traceability systems for globally traded agricultural products, with a focus on soy. Alternative traceability strategies in other sectors were also analysed. One focus was on traceability strategies for products with characteristics for which there are no analytical verification methods. Examples include imports of “conflict minerals” into the EU. The so-called EU Conflict Minerals Regulation requires importers of certain raw materials to carry out due diligence in the supply chain. Due diligence regulations, such as the EU’s Conflict Minerals Regulation, can legally oblige companies to take responsibility for certain risks in their supply chains. They can also require the importer to prove the regional origin of imported goods. The insights from those alternative traceability systems are transferred to products that might contain GMOs. When applied to the issue of GMOs, we propose reversing the burden of proof: All companies importing agricultural commodities must endeavour to identify risks of unauthorised GMOs (including NGTs) in their supply chain and, where appropriate, take measures to minimise the risk to raw material imports. The publication concludes that traceability is a means to an end and serves as a prerequisite for due diligence in order to minimise the risk of GMO contamination in supply chains. The exemplary transfer of due diligence to a company in the food industry illustrates the potential benefits of mandatory due diligence, particularly for stakeholders actively managing non-GMO supply chains. Full article
(This article belongs to the Special Issue Detection Strategies and Risk Assessment of Genetically Modified Foods)
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Other

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10 pages, 216 KiB  
Perspective
Considerations for the Successful Detection and Quantification of Genetically Modified Events in Grain and Food Samples Using Multiplex Digital PCR
by Tigst Demeke
Foods 2025, 14(1), 75; https://doi.org/10.3390/foods14010075 - 31 Dec 2024
Viewed by 1212
Abstract
The number of genetically modified (GMO) events for canola, corn, and soybean is steadily increasing. Some countries, including those in the EU, have regulatory requirements for the approval and use of plant ingredients containing GMOs. Multiplex digital PCR (dPCR) has been used for [...] Read more.
The number of genetically modified (GMO) events for canola, corn, and soybean is steadily increasing. Some countries, including those in the EU, have regulatory requirements for the approval and use of plant ingredients containing GMOs. Multiplex digital PCR (dPCR) has been used for the simultaneous detection and quantification of various GMO events. This review covers the various factors to consider for multiplex digital PCR detection and the quantification of GMO events. DNA quality, quantity, and the presence of inhibitors are important factors to consider. Some dPCR instruments allow the use of multiple fluorescent dyes, which facilitates the setup of multiplex dPCR assays. This review focuses on the optimization of multiplex dPCR and describes the multiplex dPCR assays that have been reported for GMO detection. Full article
(This article belongs to the Special Issue Detection Strategies and Risk Assessment of Genetically Modified Foods)
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