Beverages

14 pages, 1287 KiB

Open AccessArticle

The Effects of Pasteurization and Beer Type on the Functional Compounds and Flavor Substances in Beer

by Jiahui Ding, Xiaoping Hou, Jianghua Li, Xinrui Zhao and Shumin Hu

Beverages 2025, 11(3), 63; https://doi.org/10.3390/beverages11030063 - 1 May 2025

The study of functional compounds is of great importance for the production of nutrient-rich and flavorful beer. In this study, the effects of the pasteurization process and beer type (lager and ale, draft beer, and ripe beer) on the types and contents of [...] Read more.

The study of functional compounds is of great importance for the production of nutrient-rich and flavorful beer. In this study, the effects of the pasteurization process and beer type (lager and ale, draft beer, and ripe beer) on the types and contents of functional compounds were investigated, including the derivatives of amino acids (γ-aminobutyric acid and glutathione), β-glucan, phenolic acids, vitamin B, and volatile compounds. Among the six different types of beers tested, it was found that the concentration of functional compounds in ale was higher than that in lager. In addition, the process of pasteurization resulted in the loss of B vitamins and ferulic acid and an increase in some off-flavors such as aldehydes. The results of this study can aid in the development of novel functional beer and new strategies to improve beer quality. Full article

(This article belongs to the Section Malting, Brewing and Beer)

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13 pages, 10505 KiB

Open AccessArticle

L-Malic Acid Descaler for Drinking Water—Physicochemical Analysis and Biological Activity

by Teodora Todorova, Krassimir Boydzhiev, Ignat Ignatov, Teodora Petrova Popova, Zhechko Dimitrov, Irina Gotova, Fabio Huether, Alexander Ignat Ignatov and Yordan Georgiev Marinov

Beverages 2025, 11(3), 62; https://doi.org/10.3390/beverages11030062 - 29 Apr 2025

Abstract

The present study aimed to analyze the physicochemical properties and biological activity of an L-malic acid descaler. The treated water with L-malic acid descaler complies with EU Directive No. 2020/2184 for the quality of water intended for human consumption. The L-malic acid descaler [...] Read more.

The present study aimed to analyze the physicochemical properties and biological activity of an L-malic acid descaler. The treated water with L-malic acid descaler complies with EU Directive No. 2020/2184 for the quality of water intended for human consumption. The L-malic acid descaler contains L-malic acid as the active component, while polyethylene and activated charcoal function as structural and absorbent materials, respectively. The composition was analyzed in a licensed laboratory using Chemical Abstracts Service Number (CAS) and European List of Notified Chemical Substances (EINECS) standards. Fourier Transform Infrared (FT-IR) analysis confirmed the presence of hydroxyl (–OH), carbonyl (C=O), and carboxyl (–COOH) groups in L-malic acid descaler, which are connected with proton-donating ability, and redox activity. The biological activity was evaluated using Saccharomyces cerevisiae as a model system. The role of the YAP1 transcription factor, a key regulator of oxidative stress defense mechanisms, was also examined. The detrimental effects on a cellular level were induced by the well-known mutagen—methyl methanesulfonate (MMS). Our data revealed that yeast cells treated with such water decrease the MMS-induced superoxide anions (3.5-fold), total glutathione lipid peroxidation (1.5-fold), and total glutathione (3-fold) and increase cell survival (2-fold). In conclusion, water treated with L-malic acid descaler possesses antioxidant effects in yeast-cell-based tests, independent of YAP1 transcription factor activity. This study provides preliminary evidence that L-malic acid, when dissolved in water, significantly reduced MMS-induced superoxide anions, one of the biomarkers contributing to the genotoxic and carcinogenic effects of MMS. Full article

(This article belongs to the Special Issue Sports and Functional Drinks)

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11 pages, 236 KiB

Open AccessArticle

Physicochemical Properties of Traditionally Produced Mead

by Kristina Habschied, Blanka Bilić Rajs, Lorena Dozan, Vinko Krstanović and Krešimir Mastanjević

Beverages 2025, 11(3), 61; https://doi.org/10.3390/beverages11030061 - 28 Apr 2025

Abstract

Mead is described as a traditional alcoholic drink produced by fermentation from a solution of honey and water. It has been produced as a refreshing drink. However, in the past, it was more expensive than wine, which led to a decrease in demand. [...] Read more.

Mead is described as a traditional alcoholic drink produced by fermentation from a solution of honey and water. It has been produced as a refreshing drink. However, in the past, it was more expensive than wine, which led to a decrease in demand. Due to the simple method of production, the mead industry is growing again. The quality and physicochemical properties of mead depend on the type of honey used. The goal of this study is to produce mead from two kinds of honey of different floral origins, chestnut and sunflower, in order to determine the differences using sensory and physicochemical analyses. The fermentation process was monitored until the extract values were consecutively the same. The results obtained in this research indicate that chestnut honey mead took a longer time to ferment, 2 months, while sunflower honey mead took 1.5 months to ferment. The alcohol content in chestnut honey mead was 7.2% v/v, and sunflower honey mead contained 8.6% v/v. Sensory-wise, the chestnut mead was more acceptable to consumers due to a more pronounced color and thus received a one-point higher score (15) than sunflower honey mead (14). Full article

22 pages, 1906 KiB

Open AccessArticle

Impact of Foliar Application of Winemaking By-Product Extracts in Tempranillo Grapes Grown Under Warm Climate: First Results

by Zulema Piñeiro, Rocío Gutiérrez-Escobar, María Jose Aliaño-González, María Isabel Fernández-Marín, María Jesús Jiménez-Hierro, Enrico Cretazzo and Inmaculada Concepción Rodríguez-Torres

Beverages 2025, 11(3), 60; https://doi.org/10.3390/beverages11030060 - 24 Apr 2025

Abstract

The Tempranillo grape variety is the most widely cultivated red grape cultivar in Spain. However, it has been observed that this variety may not always exhibit the optimal colour properties in warm climates such as Andalusia (southern Spain). Several strategies have been proposed [...] Read more.

The Tempranillo grape variety is the most widely cultivated red grape cultivar in Spain. However, it has been observed that this variety may not always exhibit the optimal colour properties in warm climates such as Andalusia (southern Spain). Several strategies have been proposed to enhance productivity and resilience to stress or improve the quality of both grapes and wines. The foliar application of agricultural plant extracts has been identified as a highly effective method of promoting the synthesis of secondary metabolites in the grapevine. The objective of the current research was to analyse the incidence of foliar application of different extracts from winemaking by-products (grape stems: GS, grape pomaces: GP and wine lees: WL) on Tempranillo grapevine leaves, studying the impact on the quality of the resulting wines. The oenological and colour characteristics of the wines were assessed to evaluate the impact of these extracts on wines during the winemaking process. The detailed composition of the wines revealed that GS and WL extracts led to significant differences in wine colour properties. Wines derived from grapes treated with both extracts exhibited heightened colour intensity, accompanied by discernible alterations in CIELab coordinates, with lower L* and higher a* and C*ab values in comparison to CT. These results are encouraging, and the foliar application of GS extracts at veraison appears to be a feasible alternative to enhance the colour of wines from red grape cultivars with colour difficulties in warm climates. Full article

(This article belongs to the Section Wine, Spirits and Oenological Products)

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13 pages, 1171 KiB

Open AccessArticle

Development and Validation of an HPLC-DAD Method to Determine Alkylphenols in Milk

by Serenella Seccia and Irene Dini

Beverages 2025, 11(3), 59; https://doi.org/10.3390/beverages11030059 - 24 Apr 2025

Abstract

While providing considerable societal and economic benefits, plastic packaging leads to global pollution and poses health risks. Plastic additives like alkylphenols (APs) can interfere with endocrine functions even at low concentrations. Therefore, developing and validating analytical methods for their routine dosage in foods [...] Read more.

While providing considerable societal and economic benefits, plastic packaging leads to global pollution and poses health risks. Plastic additives like alkylphenols (APs) can interfere with endocrine functions even at low concentrations. Therefore, developing and validating analytical methods for their routine dosage in foods is paramount. The present work validated a chromatographic method to quantify alkylphenols (4-tert-octylphenol, 4-n-octylphenol mono-ethoxylate, 4-n-octylphenol, and 4-n-nonylphenol) in milk. The analytical method uses Chem Elut S a rapid supported liquid extraction (SLE) cartridges to eliminate the matrix effect, and reverse phase chromatography linked to a Diode Array Detector (DAD) to dosage the alkylphenols. The method was validated using the strategy of accuracy profiling, a decision-making instrument that calculates the method’s total error, encompassing bias and standard deviation. The reliability of the test was defined by the lack at the retention times of the APs of interfering peaks, the close linear relationship between the independent and the dependent variables in the regression model, the excellent precision at each concentration level for intra-day and inter-day measurements, and the errors of the procedure (systematic and random) estimated within the pre-established acceptability limits (±10%). The minimal environmental impact and ease of execution suggest its use in routine analyses. Full article

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16 pages, 1073 KiB

Open AccessArticle

Acid Adaptation Impacts Survival and Pathogenicity of Salmonella Enteritidis and Escherichia coli O157:H7 in Orange Juice

by Maria Belén Bainotti, Pilar Colás-Medà, Anna Pié-Amill, Inmaculada Viñas and Isabel Alegre

Beverages 2025, 11(3), 58; https://doi.org/10.3390/beverages11030058 - 23 Apr 2025

Abstract

Pathogenic bacteria surviving acidic conditions in foods pose a persistent public health risk, with acid adaptation potentially enhancing their virulence in low-pH environments like fruit juices. In this study, we hypothesized that foodborne bacteria exhibit enhanced survival and pathogenic potential in fruit juice [...] Read more.

Pathogenic bacteria surviving acidic conditions in foods pose a persistent public health risk, with acid adaptation potentially enhancing their virulence in low-pH environments like fruit juices. In this study, we hypothesized that foodborne bacteria exhibit enhanced survival and pathogenic potential in fruit juice after undergoing prior acid adaptation. Thus, our aim was to investigate the pathogenic potential and survival of acid-adapted Salmonella Enteritidis and Escherichia coli O157:H7 in orange juice. Cells were acid-adapted by cultivation in a glucose-rich medium, resulting in an increase in bacterial populations from 3.0–3.1 to 8.7–9.0 log₁₀ CFU/mL, compared to non-acid-adapted controls. After 2 days at the common temperature storage of the fruit juices (4 °C), the acid-adapted S. Enteritidis and E. coli survived at higher levels in orange juice compared to non-acid-adapted populations (differences of around 1.7 log units between both). Finally, acid-adapted bacteria showed greater survival during passage through simulated gastrointestinal tract conditions, indicating increased pathogenic potential. Regarding the pathogenic potential, acid-adapted E. coli O157:H7 showed an adhesion capability of around 3.5 times higher in comparison with non-acid-adapted E. coli O157:H7. These results highlight the importance of considering the adaptability of foodborne bacteria to acidic conditions in food safety and security studies. Full article

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17 pages, 2454 KiB

Open AccessArticle

Cacao Mucilage Valorisation to Produce Craft Beers: A Case Study Towards the Sustainability of the Cocoa Industry in Los Ríos Province

by José Villarroel-Bastidas, Jonathan Steven Párraga-Maquilón, Cinthya Elizabeth Zapata-Zambrano, María de Guide Córdoba, Alicia Rodríguez, Alejandro Hernández and Josué Briones-Bitar

Beverages 2025, 11(3), 57; https://doi.org/10.3390/beverages11030057 - 22 Apr 2025

Abstract

Cacao mucilage is a rich medium for microbial development due to the presence of various sugars, water, pectin, mineral salts, and yeasts of the Saccharomyces cerevisiae type. This study aims to provide added value to this commonly discarded residue, thereby contributing to the [...] Read more.

Cacao mucilage is a rich medium for microbial development due to the presence of various sugars, water, pectin, mineral salts, and yeasts of the Saccharomyces cerevisiae type. This study aims to provide added value to this commonly discarded residue, thereby contributing to the economic growth of the Rio Chila area in the Valencia Canton of Los Ríos Province. The methods applied for developing beer consist of malting, grinding, mashing, filtering, boiling, cooling, fermentation (during which cacao mucilage is added), and maturation, followed by physical–chemical analyses. The Fine aroma cacao mucilage presented values of 0.66% acidity, 7.63 °Brix, pH 4.43, absorbance 1.13, transmittance 23.67%, suspended solids 0.04 g: 2.66%, density 1.07 g/mL, turbidity 6.94 NTU, °GL 8.47% vol., foam quantity 1.70 cm, colorimetry L* 50.77, colorimetry a* 18.08, colorimetry b* 50.53, and bitterness degree 39.00. The analyses presented values within the normal parameters applied to beers at the national level (INEN standards). Escherichia coli, Salmonella, and total microorganisms showed no contamination in the microbiological analyses. In the sensory analyses, appearance, aroma, flavour, and mouthfeel were evaluated, with the best experiment being the combination of Fine aroma cacao with a concentration of 30% mucilage and added Cascade hops. This study took into account the concentrations of cacao mucilage (20% and 30%) from the varieties (Fine aroma and CCN-51), as well as the addition of the brewing hops Cascade and Northern Brewer. Regarding the physicochemical characteristics, adding this cacao derivative did not affect craft beer and conformed to the ranges of the NTE INEN 2262 standard. Thus, this research proposes an alternative use for cocoa mucilage, contributing to waste reduction and broadening its potential applications. Full article

(This article belongs to the Special Issue Developments in Brewing Processing and Analytical Techniques for the Evaluation of Beer Quality)

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16 pages, 1719 KiB

Open AccessArticle

In Vitro Screening of Potential Role of Green and Roasted Coffee Extracts in Type 2 Diabetes Management

by Lorena G. Calvo, Vinicius de Monte Vidal, Victoria Díaz-Tomé, Francisco J. Otero Espinar and Trinidad de Miguel

Beverages 2025, 11(3), 56; https://doi.org/10.3390/beverages11030056 - 22 Apr 2025

Abstract

This study evaluates the potential role of two extracts derived from green and roasted coffee in managing Type 2 Diabetes (T2D). The phytochemical analysis revealed that the roasted coffee extract (RCE) contains higher levels of flavonoids and tannins, while the green coffee extract [...] Read more.

This study evaluates the potential role of two extracts derived from green and roasted coffee in managing Type 2 Diabetes (T2D). The phytochemical analysis revealed that the roasted coffee extract (RCE) contains higher levels of flavonoids and tannins, while the green coffee extract (GCE) seems to be richer in phenolic acids. No differences in the antioxidant activity of both extracts were observed. The study demonstrated that GCE exhibits stronger prebiotic potential by significantly enhancing the growth of beneficial probiotic bacteria when compared to the untreated sample. Both extracts inhibited α-amylase and α-glucosidase enzymes, with RCE demonstrating a better performance than other commercial treatments for T2D. Glucose uptake assays on yeast cells demonstrated that both extracts enhance glucose transport, particularly at low glucose concentrations, reducing supernatant glucose levels by 60–80%. Notably, GCE maintained its effectiveness even at the highest glucose concentrations tested. These findings suggest that coffee extracts, particularly GCE, may be useful as nutraceuticals for potentially regulating glucose metabolism and gut microbiota in T2D management. Full article

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19 pages, 2095 KiB

Open AccessArticle

Development of Low-Caffeine Kombucha Using Lotus Root Tea and an Evaluation of Its Functional Properties

by Jin Seon Baek, Younhee Nam, Sunghee Kim, Hee Song Kim, Eun Jin Lee, Mee-Ryung Lee and Soo Rin Kim

Beverages 2025, 11(2), 55; https://doi.org/10.3390/beverages11020055 - 18 Apr 2025

Abstract

Kombucha, traditionally fermented from black or green tea, is well known for its potential health benefits. However, its high caffeine content may limit consumption for certain individuals. Therefore, this study aimed to develop a low-caffeine kombucha using lotus root tea as an alternative [...] Read more.

Kombucha, traditionally fermented from black or green tea, is well known for its potential health benefits. However, its high caffeine content may limit consumption for certain individuals. Therefore, this study aimed to develop a low-caffeine kombucha using lotus root tea as an alternative to black or green tea. Lotus root was roasted and brewed to prepare the tea base, to which sugar and a SCOBY were added for primary fermentation. Subsequently, Lactobacillus plantarum (1.0 × 10⁹ and 3.0 × 10⁹ CFU/mL) was inoculated to carry out secondary fermentation. The kombucha samples were assessed for their organic acid composition, antioxidant activity, antimicrobial effects, β-glucuronidase inhibition, and protective effects against Salmonella infection in a Caenorhabditis elegans model. The caffeine concentration of lotus root tea kombucha was significantly lower than that of conventional kombucha. L. plantarum fermentation increased the lactic acid concentration and enhanced antimicrobial activity, particularly against Escherichia coli OP50 and Salmonella typhimurium. Additionally, β-glucuronidase inhibition significantly improved, suggesting potential gut health benefits. In C. elegans, kombucha consumption improved survival rates following Salmonella infection, indicating a protective effect. This study demonstrates that fermentation using Lactobacillus plantarum can enhance the bioactivity of lotus root kombucha, highlighting its potential as a low-caffeine functional beverage. Full article

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14 pages, 1158 KiB

Open AccessArticle

Chemical Quality and Characterization of Essential Oils in Postharvest Hop cv. Cascade: Ventilated Room Temperature as a Sustainable Alternative to Hot-Stove and Freeze-Drying Processes

by Edoardo Monacci, Chiara Sanmartin, Alessandro Bianchi, Stefano Pettinelli, Basma Najar, Fabio Mencarelli and Isabella Taglieri

Beverages 2025, 11(2), 54; https://doi.org/10.3390/beverages11020054 - 18 Apr 2025

Abstract

Hop is a key ingredient in beer production, and drying it allows it to be stored before use. Unfortunately, postharvest drying techniques can negatively affect hop quality. In this study, we compared drying using a hot stove (H), freeze-drying (F), and ventilated at [...] Read more.

Hop is a key ingredient in beer production, and drying it allows it to be stored before use. Unfortunately, postharvest drying techniques can negatively affect hop quality. In this study, we compared drying using a hot stove (H), freeze-drying (F), and ventilated at room temperature (VRT) drying, focusing on the chemical quality and essential oil composition. To achieve 80% water removal, F and H took two days, while VRT took five days. F and H preserved a high content of total chlorophyll (F 81.89 g/kg dm; H 82.70 g/kg dm) and carotenoids (F 54.02 g/kg dm; H 54.71 g/kg dm). The hop storage index (HSI) increased with all drying techniques, but especially in F and H. The lowest amount of polyphenols was found in the H sample (348.48 g/kg dm), while the highest content was found in VRT (631.11 g/kg dm). Freeze-drying gave the best results, especially in relation to the amount of polyphenols and antioxidant power of the product. Regarding essential oils, in the class of sesquiterpene hydrocarbons, we found α-humulene (F 24.0%; VRT 24.7%; H 25.6%), β-caryophyllene (F 10.5%; VRT 9.4%; H 11.1%), and β-farnesene (F 6.8%; VRT 6.0%; H 7.4%). The total monoterpene hydrocarbon amount increased in the VRT sample. Thus, freeze-drying emerges as an alternative technique to the hot stove; however, the cost is high. Instead, drying at ventilated room temperature represents a sustainable and valid technique for preserving the aromatic characteristics and polyphenols of the product. Full article

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12 pages, 204 KiB

Open AccessArticle

Foliar Selenium Biofortification in Temperate Fruit Crops: Impact on Selenium Accumulation and Nutritional Quality of Fruits and Juices

by Ján Mezey, Ivana Mezeyová, Adrián Selnekovič and Daniel Bajčan

Beverages 2025, 11(2), 53; https://doi.org/10.3390/beverages11020053 - 18 Apr 2025

Abstract

As an essential mineral element, selenium (Se) must be consumed by organisms through food and beverages. A method used to raise the amount of Se in food made from plants is biofortification, which is the process that increases the bioactivity and content of [...] Read more.

As an essential mineral element, selenium (Se) must be consumed by organisms through food and beverages. A method used to raise the amount of Se in food made from plants is biofortification, which is the process that increases the bioactivity and content of Se in the edible parts of plants. Foliar fertilization is the most feasible method of introducing selenium into the food chain. The objective of this work was to determine the effect of foliar biofortification with Selenium on various quality attributes of fruit species suitable for fruit-based beverage production, with the main goal of verifying the incorporation of Se into plant tissues. During the growing season in 2023, sodium selenate was applied in an equivalent of 150 g/ha Se in professional raspberry, blueberry, redcurrant, honeysuckle and apple plantings, from which fruit-based juice was later produced and analyzed. There was significant increase (p ≤ 0.05) in the Se content in the fruit’s juice, which was the main goal. Furthermore, after the application of Se under the mentioned conditions, there was a significant (p ≤ 0.05) increase in nutritionally valuable parameters, such as antioxidant activity, ferulic acid and resveratrol, but also the content of glucose, fructose, malic acid, total acids, Mn, Ba, Ca, Li, myricetin and chlorogenic acid content. On the other hand, a decrease in some valuable indicators, but also heavy metals (Al, Cu, Cr), were noticed in some fruit juices. Full article

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18 pages, 1824 KiB

Open AccessArticle

Effect of Partial Condensation (Dephlegmation) in Fruit Brandy Distillation Equipment on the Composition of Apple Brandies

by Manami Yagishita, Oliver Reber, Daniela Alter, Ralf Kölling and Daniel Einfalt

Beverages 2025, 11(2), 52; https://doi.org/10.3390/beverages11020052 - 17 Apr 2025

Abstract

Fruit brandy equipment commonly uses partial condensation (dephlegmation) to generate reflux in the distillation column. Here, we examined the effect of dephlegmation on the composition of fruit brandies in both lab-scale and large-scale settings. In lab-scale experiments, the dephlegmator led to a pronounced [...] Read more.

Fruit brandy equipment commonly uses partial condensation (dephlegmation) to generate reflux in the distillation column. Here, we examined the effect of dephlegmation on the composition of fruit brandies in both lab-scale and large-scale settings. In lab-scale experiments, the dephlegmator led to a pronounced enrichment of ethanol in the distillate due to preferred condensation of water, while the concentration of flavor compounds was differentially affected. Some compounds were enriched in the distillate, some were depleted, and some were unaffected by dephlegmation compared with the control without a dephlegmator. Large-scale fruit brandy equipment relying exclusively on dephlegmation was compared as standard with an enrichment section containing three trays. In the equipment relying on dephlegmation, tail components such as fusel alcohols were less well separated from the middle run, which led to a reduced yield of clean spirit in the middle run. In triangle tests, the spirits from the two devices could be clearly differentiated, but there was no clear preference for one spirit or the other. This study provides for the first time detailed data on the influence of dephlegmators on the behavior of flavor compounds during fruit brandy distillation. Full article

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13 pages, 579 KiB

Open AccessArticle

Development of a Citrus Drink Using Mixture Design: Sensory Evaluation, Total Polyphenols and Vitamin C

by Jeimison Bazán-Plasencia, Antony Mejía-Vásquez, Gerald H. Bracamonte, Juanita Anali Ponce-Ramirez, Ronald Ortecho-Llanos, Beetthssy Z. Hurtado-Soria, Eudes Villanueva and Elza Aguirre

Beverages 2025, 11(2), 51; https://doi.org/10.3390/beverages11020051 - 15 Apr 2025

Abstract

The development of beverages with functional potential and maximum sensory acceptability are priorities for the food industry. This study investigated the effect of orange juice (OJ), lemon juice (LJ) and ginger juice (GJ) concentrations on overall acceptability (OA), total phenic compounds (TPC) and [...] Read more.

The development of beverages with functional potential and maximum sensory acceptability are priorities for the food industry. This study investigated the effect of orange juice (OJ), lemon juice (LJ) and ginger juice (GJ) concentrations on overall acceptability (OA), total phenic compounds (TPC) and vitamin C (VC). The experimental design used was simplex-centroid mixtures, and maximization was achieved using response surface methodology (RSM). The bioactive content of TPC and VC was measured using the Folin–Ciocalteu method and the dichlorophenol indophenol method, respectively; sensory evaluation was conducted with 100 panelists on a continuous scale acceptability primer (0–10 points). The RSM results indicated that the optimum formulation was obtained with: OJ = 79.8 mL, LJ = 5.7 mL and GJ = 4.37 mL, yielding maximum values of OA = 6.81 points, TPC = 14.64 mg GAE/100 g and VC = 32.3 mg/mL. The optimized beverage presented a free radical scavenging capacity of 65.72% (DPPH method). The regression models were validated and significantly (p < 0.05) predicted sensory acceptability, total phenolic compounds and vitamin C content. The development of this beverage and its potential application in the food industry is attractive due to its high sensory acceptability and bioactive content. Full article

(This article belongs to the Section Sensory Analysis of Beverages)

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15 pages, 794 KiB

Open AccessArticle

CLA-Producing Probiotics for the Development of a Yogurt-Type Beverage

by Hasnia Ziar, Philippe Gérard and Ali Riazi

Beverages 2025, 11(2), 50; https://doi.org/10.3390/beverages11020050 - 14 Apr 2025

Abstract

This study examined the ability of four beneficial strains (Lactobacillus rhamnosus LbRE-LSAS, Bifidobacterium animalis subsp. lactis Bb12, and two yogurt starters TA040 and LB340) to ferment MRS or milk containing free linoleic acid (0, 0.5, or 1 mg/mL). The goal was to produce [...] Read more.

This study examined the ability of four beneficial strains (Lactobacillus rhamnosus LbRE-LSAS, Bifidobacterium animalis subsp. lactis Bb12, and two yogurt starters TA040 and LB340) to ferment MRS or milk containing free linoleic acid (0, 0.5, or 1 mg/mL). The goal was to produce an enriched conjugated linoleic acid (CLA) isomers’ yogurt-type beverage. Linoleic acid (LA) at 0.5 mg/mL did not interfere with the growth of the assayed bacteria on de Man Rogosa and Sharpe broth (MRS) or milk. On the other hand, increasing the content of LA in the MRS or yogurt-type beverage to 1 mg/mL slightly inhibited all strains and prevented accumulating high biomasses. A gas chromatography analysis of the fatty acid profiles confirmed the bioconversion of LA. The yogurt starters TA040 and LB340 had the highest bioconversion rates in the yogurt-type beverages, whereas the probiotic Bb12 strain was the most interesting at converting LA into its active CLA. CLA from the MRS supernatants of TA040, Bb12, and LbRE-LSAS had maximum antibacterial activities against S. typhimurium, E. coli, and S. aureus, respectively. Whey from the Bb12 beverage showed an inhibitory effect against all pathogens. These results suggest that all strains could be used as starter cultures in the proposition of a yogurt-type beverage with a high CLA content and antibacterial potential. Full article

(This article belongs to the Section Beverage Technology Fermentation and Microbiology)

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20 pages, 893 KiB

Open AccessReview

Fermented Cashew Apple Beverages: Current State of Knowledge and Prospects

by Fabrice S. Codjia, D. Sylvain Dabadé, Pélagie Agbobatinkpo, Ingrid Collombel, Nawel Achir, Paulin Azokpota and Joseph Dossou

Beverages 2025, 11(2), 49; https://doi.org/10.3390/beverages11020049 - 14 Apr 2025

Abstract

The cashew apple constitutes approximately 90% of the total fruit mass produced by the cashew tree, with the remaining 10% being the cashew nut. Despite its high nutritional value, it is regarded as an agricultural byproduct. Numerous scientific studies have explored the technological [...] Read more.

The cashew apple constitutes approximately 90% of the total fruit mass produced by the cashew tree, with the remaining 10% being the cashew nut. Despite its high nutritional value, it is regarded as an agricultural byproduct. Numerous scientific studies have explored the technological and nutritional potential of the cashew apple by leveraging microorganisms in its fermentation process for beverage applications. This paper provides an overview of existing fermented cashew apple beverages and discusses perspectives for new fermented cashew apple products. Five fermented cashew apple beverages were recorded. These include wine, edible alcohol, probiotic and prebiotic beverages, and cashew apple-based vinegar. New fermented cashew apple beverages with organoleptic, nutritional, and functional properties can be considered. Among these are fermented cashew apple beverages such as kefir or kombucha-type drinks. A promising avenue for future research is the exploration of the indigenous microbiota of the cashew apple and their interactions within a consortium. This could lead to innovative developments in food technology and improvements in the organoleptic and nutritional characteristics of fermented cashew apple beverages. Full article

(This article belongs to the Section Beverage Technology Fermentation and Microbiology)

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15 pages, 1067 KiB

Open AccessArticle

Multi-Analytical Characterisation of an Alcoholic Beverage Obtained by Blending of White Wine and Organic Kiwifruit Wine

by Lorenzo Marsili, Fabio Pietrangeli, Claudio Brilli, Martina Foschi, Alessandra Biancolillo, Angelo Antonio D’Archivio and Guerino Pescara

Beverages 2025, 11(2), 48; https://doi.org/10.3390/beverages11020048 - 7 Apr 2025

Abstract

While studies on the co-fermentation of fruits and grapes are reported in the literature, blends of wine and fruit wine have never been investigated before. We characterised a blend (80:20 v/v) of Trebbiano Abruzzese white wine, organic kiwifruit wine, and [...] Read more.

While studies on the co-fermentation of fruits and grapes are reported in the literature, blends of wine and fruit wine have never been investigated before. We characterised a blend (80:20 v/v) of Trebbiano Abruzzese white wine, organic kiwifruit wine, and the sparkling beverage obtained by its successive refermentation in a bottle. A persimmon/kiwifruit wine (50:50 v/v), after and before alcoholic fermentation, was also analysed. The buffer capacity, redox potential, concentration of selected polyphenols, total polyphenol content, antioxidant activity, and volatile profile were evaluated. The addition of kiwifruit wine to Trebbiano Abruzzese confers, to the final beverage, an appreciable improvement in terms of antioxidant activity, related to the content of polyphenols and ascorbic acid, which is substantially preserved in the sparkling product. Appreciable differences in the aroma of the blend compared to pure wine are mainly associated with the increased content of norisoprenoids, terpenes, methyl esters, and sulphur compounds, arising from the kiwifruit wine. The innovative beverages here proposed exhibit distinctive compositional and sensorial attributes that can be appreciated by consumers. Full article

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19 pages, 968 KiB

Open AccessArticle

Characterization of Commercial Non-Alcoholic Beers in Two Locations, Finland and China

by Niina Kelanne, Yuxuan Zhu, Tuomas Rysä, Baoru Yang, Baoqing Zhu and Oskar Laaksonen

Beverages 2025, 11(2), 47; https://doi.org/10.3390/beverages11020047 - 29 Mar 2025

Abstract

Consumption of non-alcoholic beer (NAB) is continuously increasing in many countries. NABs are produced with varying technologies, resulting in different sensory properties. Previous studies have focused on understanding the consumers’ acceptance and preferences among different types of NAB. However, few studies have focused [...] Read more.

Consumption of non-alcoholic beer (NAB) is continuously increasing in many countries. NABs are produced with varying technologies, resulting in different sensory properties. Previous studies have focused on understanding the consumers’ acceptance and preferences among different types of NAB. However, few studies have focused on the sensory characterization of commercial NABs produced with different methods. This study aimed to find key sensory characteristics in lager, Indian pale ale, and pilsner-type NABs with untrained panels by conducting tests in Finland and China. Participants were asked to Check-All-That-Apply for odors and Rate-All-That-Apply for flavors. Additionally, hedonic responses to odor and flavor pleasantness were rated. Chinese participants used more different and more often-used attributes to describe NABs. More varying descriptors were used on the NAB samples chosen as the most pleasant ones, indicating more complex sensory properties. Participants from both locations preferred lager-type beers characterized as ‘fresh’ odor and ‘fruity’, ‘floral’, and ‘sweet’ flavors and less frequently found Indian-pale-ale-type NABs pleasant, describing them as ‘sour’, ‘bitter’, ‘fermented’, and ‘pungent’ flavors. The flavor pleasantness correlated more with the samples frequently chosen as the most pleasant than odor pleasantness. No clear connection was found between the production method of NAB and consumers’ acceptance, demonstrating that all production methods can be used to produce non-alcoholic beers with sensory properties acceptable to consumers. Full article

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16 pages, 1425 KiB

Open AccessSystematic Review

Total Water Intake and Total Fluid Intake Worldwide: A Systematic Literature Review in Children and Adolescents

by Georgios Papaoikonomou, Kyriaki Apergi and Olga Malisova

Beverages 2025, 11(2), 46; https://doi.org/10.3390/beverages11020046 - 27 Mar 2025

Abstract

Background: Investigating fluid consumption among children and adolescents poses a challenge due to varying dietary behaviors and hydration needs. This systematic review aims to assess total water intake (TWI) and total fluid intake (TFI) in children and adolescents, focusing on gender differences and [...] Read more.

Background: Investigating fluid consumption among children and adolescents poses a challenge due to varying dietary behaviors and hydration needs. This systematic review aims to assess total water intake (TWI) and total fluid intake (TFI) in children and adolescents, focusing on gender differences and adherence to reference values proposed by the European Food Safety Authority (EFSA) and the Institute of Medicine (IOM). Methods: A systematic literature search of PubMed, Scopus, and Scholar was conducted to identify studies published between 2004 and 2024 reporting on TWI and TFI for children and adolescents. Studies were included if they reported fluid intake data in healthy populations, provided quantitative measures of TWI or TFI, and aligned with the EFSA or IOM reference values. From 8731 initial articles, 24 studies met the inclusion criteria. Data were synthesized narratively, and compliance with hydration guidelines was assessed. Results: The review included 24 studies, encompassing 16,254 children and 15,367 adolescents. The majority of participants failed to meet the recommended guidelines. Only one study reported compliance with the recommended TWI values, while four studies in children and four in adolescents showed adherence to the recommended TFI values. Conclusions: The results underscore a widespread inadequacy in achieving the hydration guidelines among children and adolescents, emphasizing the need for targeted interventions to improve fluid intake. Public health interventions are needed to promote adequate fluid intake, particularly in populations at risk of dehydration-related health outcomes. Future research should focus on identifying barriers to adequate hydration and developing targeted strategies to improve fluid intake behaviors. Full article

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14 pages, 3341 KiB

Open AccessArticle

Applications of Machine Learning for Wine Recognition Based on ¹H-NMR Spectroscopy

by Ariana Raluca Hategan, Adrian Pirnau and Dana Alina Magdas

Beverages 2025, 11(2), 45; https://doi.org/10.3390/beverages11020045 - 27 Mar 2025

Cited by 1

Abstract

The present study aims to explore the possibility of applying machine learning (ML) algorithms for the development of reliable wine authentication instruments able to encompass the exhaustive performances offered by learning-based methods, and, at the same time, to propose reference practices for improving [...] Read more.

The present study aims to explore the possibility of applying machine learning (ML) algorithms for the development of reliable wine authentication instruments able to encompass the exhaustive performances offered by learning-based methods, and, at the same time, to propose reference practices for improving the recognition ability of the developed models. In this regard, two ML algorithms, namely k-Nearest Neighbors (kNN) and Logistic Regression, have been utilized as supervised learning techniques applied to ¹H-NMR spectral data for the development of classification models able to recognize the variety, geographical origin, and vintage of wine samples. Due to the complexity of the experimental data, which was characterized by a high number of variables, special attention was given to the preprocessing phase in order to identify the most relevant input space for each envisaged classification criterion. The obtained results have shown that ¹H-NMR spectroscopy in conjunction with Logistic Regression represents a reliable approach for wine traceability, leading, for all investigated classification criteria, to accuracy scores of the developed models greater than 98% in cross-validation and up to 100% in testing. Full article

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