- 4.8Impact Factor
- 9.2CiteScore
- 20 daysTime to First Decision
Biomolecules, Volume 9, Issue 6
June 2019 - 47 articles
Cover Story: Cupin-type phosphoglucose isomerase (PfPGI) from the hyperthermophilic archaeon Pyrococcus furiosus catalyzes the reversible isomerization of glucose-6-phosphate to fructose-6-phosphate. Using a 3DM-based bioinformatics approach, a pair of amino acids in the periphery of the PfPGI structure was found to be the dominant co-evolving mutation. We designed a small smart library of variants to test their biochemical activity. Although the mutant structures and metal cofactor coordination were strikingly similar, variations in their activity were observed. Using the water tracking approach, we found that the subtle changes in the structure and dynamics of the amino acid side chains might have influenced regulation of solvent access in the vicinity of the catalytic Mn2+ ion and contributed to the mutants’ activity. View this paper.
- Issues are regarded as officially published after their release is announced to the table of contents alert mailing list .
- You may sign up for email alerts to receive table of contents of newly released issues.
- PDF is the official format for papers published in both, html and pdf forms. To view the papers in pdf format, click on the "PDF Full-text" link, and use the free Adobe Reader to open them.
Articles
There are no articles in this issue yet.
Get Alerted
Add your email address to receive forthcoming issues of this journal.
Biomolecules - ISSN 2218-273X