Background: The Quantitative Analysis of Multi-components by Single-marker (QAMS) method has been developed as an alternative to the External Standards Method (ESM) for the quality control of medicinal herbs.
Objectives: In this study, QAMS was developed to determine saponins in the
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Background: The Quantitative Analysis of Multi-components by Single-marker (QAMS) method has been developed as an alternative to the External Standards Method (ESM) for the quality control of medicinal herbs.
Objectives: In this study, QAMS was developed to determine saponins in the raw materials of
Panax vietnamensis using HPLC-PDA/ELSD.
Methods: The method was developed and validated. The relative conversion factors F
x were calculated based on the linear regression for HPLC-PDA and the logarithm equation for HPLC-ELSD. The Standard Method Difference (SMD) was determined to indicate the difference in the results of QAMS and EMS.
Results: Relative conversion factors (F
x) were determined for each detector to quantify five saponins (ginsenoside Rb
1, Rd, Rg
1, majnoside R2, and vina-ginsenoside R2) in VG root. The F
x values were calculated based on the ratio of the slopes of the regression equations of a single standard and an external standard. For HPLC-PDA, G-Rb1 was used as a single standard with the F
x values of 1.00 (G-Rb
1), 1.08 (G-Rd), 1.32 (G-Rg
1), and 0.04 (M-R2). For HPLC-ELSD, G-Rb
1 was used for determining the content of G-Rg
1 and G-Rb
1 with the F
x values of 1.00 (G-Rb
1) and 0.95 (G-Rg
1), while M-R2 was used for quantitating M-R2 and V-R2 with F
x of 1.00 (M-R2) and 1.05 (V-R2). An SMD value less than 5.00% confirms the close alignment of the QAMS method with ESM.
Conclusions: The QAMS method proved to be a feasible and promising method for the quality control of
P. vietnamensis.
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